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Epigenetics and Nuclear Signaling Histones H4 Biotinylated
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Anti-Histone H4 (biotinylated K5) antibody (ab17343)

  • Datasheet
  • SDS
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Western blot - Anti-Histone H4 (biotinylated K5) antibody (ab17343)

    Key features and details

    • Rabbit polyclonal to Histone H4 (biotinylated K5)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human Histone H4 protein (ab198115)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-Histone H4 (biotinylated K5) antibody
      See all Histone H4 primary antibodies
    • Description

      Rabbit polyclonal to Histone H4 (biotinylated K5)
    • Host species

      Rabbit
    • Specificity

      ab17343 specifically recognise biotinylated histone H4 K5 in HeLa nuclear lysates. ab17343 could not detect biotinylated histone H4 K5 in HeLa whole cell lysates.
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Human Histone H4 aa 1-100 (N terminal) (biotinylated K5) conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab17632)

    • Positive control

      • Hela nuclear extract
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Histones
      • H4
      • Biotinylated

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Histone H4 protein (ab198115)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab17343 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).

    Target

    • Function

      Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
    • Sequence similarities

      Belongs to the histone H4 family.
    • Post-translational
      modifications

      Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
      Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
      Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
      Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
      Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
      Sumoylated, which is associated with transcriptional repression.
    • Cellular localization

      Nucleus. Chromosome.
    • Target information above from: UniProt accession P62805 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 121504 Human
      • Entrez Gene: 554313 Human
      • Entrez Gene: 8294 Human
      • Entrez Gene: 8359 Human
      • Entrez Gene: 8360 Human
      • Entrez Gene: 8361 Human
      • Entrez Gene: 8362 Human
      • Entrez Gene: 8363 Human
      • Entrez Gene: 8364 Human
      • Entrez Gene: 8365 Human
      • Entrez Gene: 8366 Human
      • Entrez Gene: 8367 Human
      • Entrez Gene: 8368 Human
      • Entrez Gene: 8370 Human
      • Omim: 142750 Human
      • Omim: 602822 Human
      • Omim: 602823 Human
      • Omim: 602824 Human
      • Omim: 602825 Human
      • Omim: 602826 Human
      • Omim: 602827 Human
      • Omim: 602828 Human
      • Omim: 602829 Human
      • Omim: 602830 Human
      • Omim: 602831 Human
      • Omim: 602833 Human
      • SwissProt: P62805 Human
      • Unigene: 143080 Human
      • Unigene: 247816 Human
      • Unigene: 248172 Human
      • Unigene: 248178 Human
      • Unigene: 248179 Human
      • Unigene: 278483 Human
      • Unigene: 46423 Human
      • Unigene: 528055 Human
      • Unigene: 533295 Human
      • Unigene: 55468 Human
      • Unigene: 591790 Human
      • Unigene: 655235 Human
      • Unigene: 662174 Human
      • Unigene: 706635 Human
      • Unigene: 718935 Human
      see all
    • Alternative names

      • dJ160A22.1 antibody
      • dJ160A22.2 antibody
      • dJ221C16.1 antibody
      • dJ221C16.9 antibody
      • FO108 antibody
      • H4 antibody
      • H4.k antibody
      • H4/a antibody
      • H4/b antibody
      • H4/c antibody
      • H4/d antibody
      • H4/e antibody
      • H4/g antibody
      • H4/h antibody
      • H4/I antibody
      • H4/j antibody
      • H4/k antibody
      • H4/m antibody
      • H4/n antibody
      • H4/p antibody
      • H4_HUMAN antibody
      • H4F2 antibody
      • H4F2iii antibody
      • H4F2iv antibody
      • H4FA antibody
      • H4FB antibody
      • H4FC antibody
      • H4FD antibody
      • H4FE antibody
      • H4FG antibody
      • H4FH antibody
      • H4FI antibody
      • H4FJ antibody
      • H4FK antibody
      • H4FM antibody
      • H4FN antibody
      • H4M antibody
      • HIST1H4A antibody
      • HIST1H4B antibody
      • HIST1H4C antibody
      • HIST1H4D antibody
      • HIST1H4E antibody
      • HIST1H4F antibody
      • HIST1H4H antibody
      • HIST1H4I antibody
      • HIST1H4J antibody
      • HIST1H4K antibody
      • HIST1H4L antibody
      • HIST2H4 antibody
      • HIST2H4A antibody
      • Hist4h4 antibody
      • Histone 1 H4a antibody
      • Histone 1 H4b antibody
      • Histone 1 H4c antibody
      • Histone 1 H4d antibody
      • Histone 1 H4e antibody
      • Histone 1 H4f antibody
      • Histone 1 H4h antibody
      • Histone 1 H4i antibody
      • Histone 1 H4j antibody
      • Histone 1 H4k antibody
      • Histone 1 H4l antibody
      • Histone 2 H4a antibody
      • histone 4 H4 antibody
      • Histone H4 antibody
      • MGC24116 antibody
      see all

    Images

    • Western blot - Anti-Histone H4 (biotinylated K5) antibody (ab17343)
      Western blot - Anti-Histone H4 (biotinylated K5) antibody (ab17343)
      ab17343 specifically recognises biotinylated histone H4 K5 at 11 kDa, which is blocked using the immunising peptide (ab17632) but not the corresponding unmodified peptide (ab15825).

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab17343? Please let us know so that we can cite the reference in this datasheet.

    ab17343 has been referenced in 1 publication.

    • Fan P  et al. Overexpressed histone acetyltransferase 1 regulates cancer immunity by increasing programmed death-ligand 1 expression in pancreatic cancer. J Exp Clin Cancer Res 38:47 (2019). PubMed: 30709380

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