Key features and details
- Rabbit polyclonal to Histone H4 (butyryl K16)
- Suitable for: WB, ICC/IF, ChIP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Histone H4 (butyryl K16) antibody
See all Histone H4 primary antibodies
DescriptionRabbit polyclonal to Histone H4 (butyryl K16)
Tested applicationsSuitable for: WB, ICC/IF, ChIPmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Histone H4 (butyryl K16).
Database link: P62805
- WB: HEK-293 and K562 whole cell lysates treated with 30mM sodium butyrate for 4 hours. ICC/IF: HeLa cells treated with 30mM sodium butyrate for 4 hours. ChIP: Chromatin prepared from HeLa cells treated with 30mM sodium butyrate for 4 hours.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab240615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 11 kDa.|
|ICC/IF||1/50 - 1/200.|
|ChIP||Use at an assay dependent concentration.
Use 5 µg for 4 x 106 cells.
FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similaritiesBelongs to the histone H4 family.
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- dJ160A22.1 antibody
- dJ160A22.2 antibody
- dJ221C16.1 antibody
All lanes : Anti-Histone H4 (butyryl K16) antibody (ab240615) at 1/100 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 3 : HEK-293 whole cell lysate, untreated (-)
Lane 4 : K562 whole cell lysate, untreated (-)
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 11 kDa
HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) labeling Histone H4 (butyryl K16) with ab240615 at 1/50 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
HeLa (human epithelial cell line from cervix adenocarcinoma; 4 x 106, treated with 30mM sodium butyrate for 4 hours) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5 μg ab240615 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240615 has not yet been referenced specifically in any publications.