Anti-Histone H4 (butyryl K8) antibody (ab241246)
Key features and details
- Rabbit polyclonal to Histone H4 (butyryl K8)
- Suitable for: ChIP, WB, ICC, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H4 (butyryl K8) antibody
See all Histone H4 primary antibodies -
Description
Rabbit polyclonal to Histone H4 (butyryl K8) -
Host species
Rabbit -
Tested applications
Suitable for: ChIP, WB, ICC, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Histone H4 (butyryl K8).
Database link: P62805 -
Positive control
- WB: HeLa, Jurkat, HEK-293 and HepG2 whole cell lysates treated with 30mM sodium butyrate for 4 hours. ICC/IF: HeLa cells treated with 30mM sodium butyrate for 4 hours. ICC: HeLa cells treated with 30mM sodium butyrate for 4 hours. ChIP: Chromatin prepared from HeLa cells treated with 30mM sodium butyrate for 4 hours.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab241246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ChIP | Use at an assay dependent concentration. Use 5 µg for 4 x 106 cells. |
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| WB | 1/1000 - 1/5000. | |
| ICC | 1/20 - 1/200. | |
| ICC/IF | 1/20 - 1/200. |
Target
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Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
Sequence similarities
Belongs to the histone H4 family. -
Post-translational
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 121504 Human
- Entrez Gene: 554313 Human
- Entrez Gene: 8294 Human
- Entrez Gene: 8359 Human
- Entrez Gene: 8360 Human
- Entrez Gene: 8361 Human
- Entrez Gene: 8362 Human
- Entrez Gene: 8363 Human
see all -
Alternative names
- dJ160A22.1 antibody
- dJ160A22.2 antibody
- dJ221C16.1 antibody
see all
Images
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Western blot - Anti-Histone H4 (butyryl K8) antibody (ab241246)All lanes : Anti-Histone H4 (butyryl K8) antibody (ab241246) at 1/2000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours
Lane 5 : HeLa whole cell lysate, untreated (-)
Lane 6 : Jurkat whole cell lysate, untreated (-)
Lane 7 : HEK-293 whole cell lysate, untreated (-)
Lane 8 : HepG2 whole cell lysate, untreated (-)
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/40000 dilution -
Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (butyryl K8) antibody (ab241246)HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) labeling Histone H4 (butyryl K8) with ab241246 at 1/37.5 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
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Immunocytochemistry - Anti-Histone H4 (butyryl K8) antibody (ab241246)HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) labeling Histone H4 (butyryl K8) with ab241246 at 1/75 dilution in ICC.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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ChIP - Anti-Histone H4 (butyryl K8) antibody (ab241246)HeLa (human epithelial cell line from cervix adenocarcinoma; 4 x 106, treated with 30mM sodium butyrate for 4 hours) were treated with micrococcal nuclease, sonicated, and immunoprecipitated with 5 μg ab241246 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab241246 has not yet been referenced specifically in any publications.
