Recombinant Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)
![Western blot - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)](https://www.abcam.com/ps/products/177/ab177844/Images/ab177844-243068-anti-histone-h4-crotonyl-k5-antibody-epr17903-western-blot.jpg "Western blot - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17903] to Histone H4 (crotonyl K5)
- Suitable for: ICC/IF, IHC-P, WB, Dot blot
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Histone H4 (crotonyl K5) antibody [EPR17903]
See all Histone H4 primary antibodies -
Description
Rabbit monoclonal [EPR17903] to Histone H4 (crotonyl K5) -
Host species
Rabbit -
Specificity
ab177844 also shows binding to Histone H4 (crotonyl K8) when using high concentrations of a modified peptide (please see the dot-blot image on this datasheet for more information). -
Tested applications
Suitable for: ICC/IF, IHC-P, WB, Dot blotmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Histone H4 aa 1-100 (crotonyl K5). The exact sequence is proprietary.
Database link: P62805 -
Positive control
- WB: HeLa and NIH/3T3 cell lysates. IHC-P: Human stomach, mouse liver and rat pancreas tissues. ICC/IF: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17903 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab177844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/250. | |
| IHC-P | 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| WB | 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). | |
| Dot blot | 1/1000. |
Target
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Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
Sequence similarities
Belongs to the histone H4 family. -
Post-translational
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 121504 Human
- Entrez Gene: 554313 Human
- Entrez Gene: 8294 Human
- Entrez Gene: 8359 Human
- Entrez Gene: 8360 Human
- Entrez Gene: 8361 Human
- Entrez Gene: 8362 Human
- Entrez Gene: 8363 Human
see all -
Alternative names
- dJ160A22.1 antibody
- dJ160A22.2 antibody
- dJ221C16.1 antibody
see all
Images
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Western blot - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)All lanes : Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844) at 1/1000 dilution
Lane 1 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 10 secondsBlocking/Dilution buffer: 5% BSA /TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)](https://www.abcam.com/ps/products/177/ab177844/Images/ab177844-251628-anti-histone-h4-crotonyl-k5-antibody-epr17903-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H4 (crotonyl K5 + K8) with ab177844 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab177844 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution. -
![Dot Blot - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)](https://www.abcam.com/ps/products/177/ab177844/Images/ab177844-243074-anti-histone-h4-crotonyl-k5-antibody-epr17903-dot-blot.jpg)
Dot Blot - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)Dot blot analysis of crotonyl peptide (crotonyl K5) (Lane 1), crotonyl peptide (crotonyl K8) (Lane 2) and non- crotonyl peptide (Lane 3), labeled using ab177844 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)](https://www.abcam.com/ps/products/177/ab177844/Images/ab177844-243067-anti-histone-h4-crotonyl-k5-antibody-epr17903-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Histone H4 (crotonyl K5 + K8) with ab177844 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on Human stomach tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)](https://www.abcam.com/ps/products/177/ab177844/Images/ab177844-243066-anti-histone-h4-crotonyl-k5-antibody-epr17903-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Histone H4 (crotonyl K5 + K8) with ab177844 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on mouse liver tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)](https://www.abcam.com/ps/products/177/ab177844/Images/ab177844-243065-anti-histone-h4-crotonyl-k5-antibody-epr17903-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Histone H4 (crotonyl K5 + K8) with ab177844 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on rat pancreas tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)](https://www.abcam.com/ps/products/177/ab177844/Images/ab177844-8-benefits-of-recombinant-antibodies.png)
Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab177844 has not yet been referenced specifically in any publications.
