Recombinant
RabMAb

Recombinant Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)

Submit a review Submit a question

Overview

  • Product name

    Anti-Histone H4 (crotonyl K5) antibody [EPR17903]
    See all Histone H4 primary antibodies

  • Description

    Rabbit monoclonal [EPR17903] to Histone H4 (crotonyl K5)

  • Host species

    Rabbit

  • Specificity

    ab177844 also shows binding to Histone H4 (crotonyl K8) when using high concentrations of a modified peptide (please see the dot-blot image on this datasheet for more information).

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, Dot blotmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Histone H4 aa 1-100 (crotonyl K5). The exact sequence is proprietary.
    Database link: P62805

  • Positive control

    • WB: HeLa and NIH/3T3 cell lysates. IHC-P: Human stomach, mouse liver and rat pancreas tissues. ICC/IF: HeLa cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17903

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
Dot blot 1/1000.

Target

Images

  • Western blot - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)
    Western blot - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)
    All lanes : Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844) at 1/1000 dilution

    Lane 1 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% BSA /TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H4 (crotonyl K5 + K8) with ab177844 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab177844 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

  • Dot Blot - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)
    Dot Blot - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)

    Dot blot analysis of crotonyl peptide (crotonyl K5) (Lane 1), crotonyl peptide (crotonyl K8) (Lane 2) and non- crotonyl peptide (Lane 3), labeled using ab177844 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Histone H4 (crotonyl K5 + K8) with ab177844 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on Human stomach tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Histone H4 (crotonyl K5 + K8) with ab177844 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on mouse liver tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (crotonyl K5) antibody [EPR17903] (ab177844)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Histone H4 (crotonyl K5 + K8) with ab177844 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on rat pancreas tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab177844 has not yet been referenced specifically in any publications.

Publishing research using ab177844? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab177844.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com