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  1. Link

    histone-h4-di-methyl-k20-antibody-ab9052.pdf

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Epigenetics and Nuclear Signaling Histones H4 Methylated
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Anti-Histone H4 (di methyl K20) antibody (ab9052)

  • Datasheet
Reviews (11) Submit a question References (46)

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Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (di methyl K20) antibody (ab9052)
  • Immunoprecipitation - Anti-Histone H4 (di methyl K20) antibody (ab9052)
  • Western blot - Anti-Histone H4 (di methyl K20) antibody (ab9052)
  • Western blot - Anti-Histone H4 (di methyl K20) antibody (ab9052)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (di methyl K20) antibody (ab9052)

Key features and details

  • Rabbit polyclonal to Histone H4 (di methyl K20)
  • Suitable for: IP, WB, ChIP, ICC/IF, IHC-P
  • Reacts with: Mouse, Cow, Human, Drosophila melanogaster, Schizosaccharomyces pombe, Toxoplasma gondii
  • Isotype: IgG

You may also be interested in

Primary
Product image
Anti-Histone H4 (tri methyl K20) antibody [EPR17001(2)] - ChIP Grade (ab177190)
Protein
Product image
Recombinant Human Histone H4 protein (ab198115)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Histone H4 (di methyl K20) antibody
    See all Histone H4 primary antibodies
  • Description

    Rabbit polyclonal to Histone H4 (di methyl K20)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, ChIP, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Cow, Human, Drosophila melanogaster, Schizosaccharomyces pombe, Toxoplasma gondii
    Predicted to work with: Rat, a wide range of other speciesDoes not react with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Calf thymus histone preparation and HeLa whole cell extract and S. pombe. IHC-P: FFPE human pancreas adenocarcinoma tissue. ICC/IF: HeLa cell line
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H4
    • Methylated

Associated products

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Histone H4 protein (ab198115)
  • Related Products

    • Human Histone H4 peptide (ab13843)
    • Human Histone H4 (symmetric di methyl R3) peptide (ab14791)
    • Human Histone H4 (tri methyl K20) peptide (ab17567)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at an assay dependent concentration.
WB (5)
1/2000. Detects a band of approximately 11 kDa.
ChIP (1)
Use at an assay dependent concentration. PubMed: 20829797
ICC/IF (2)
Use a concentration of 1 µg/ml.
IHC-P (2)
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
IP
Use at an assay dependent concentration.
WB
1/2000. Detects a band of approximately 11 kDa.
ChIP
Use at an assay dependent concentration. PubMed: 20829797
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities

    Belongs to the histone H4 family.
  • Post-translational
    modifications

    Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
    Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
    Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
    Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
    Sumoylated, which is associated with transcriptional repression.
  • Cellular localization

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P62805 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 318846 Drosophila melanogaster
    • Entrez Gene: 3771854 Drosophila melanogaster
    • Entrez Gene: 3771893 Drosophila melanogaster
    • Entrez Gene: 3771908 Drosophila melanogaster
    • Entrez Gene: 3771935 Drosophila melanogaster
    • Entrez Gene: 3771938 Drosophila melanogaster
    • Entrez Gene: 3771941 Drosophila melanogaster
    • Entrez Gene: 3771947 Drosophila melanogaster
    • Entrez Gene: 3772096 Drosophila melanogaster
    • Entrez Gene: 3772113 Drosophila melanogaster
    • Entrez Gene: 3772129 Drosophila melanogaster
    • Entrez Gene: 3772172 Drosophila melanogaster
    • Entrez Gene: 3772211 Drosophila melanogaster
    • Entrez Gene: 3772254 Drosophila melanogaster
    • Entrez Gene: 3772314 Drosophila melanogaster
    • Entrez Gene: 3772317 Drosophila melanogaster
    • Entrez Gene: 3772319 Drosophila melanogaster
    • Entrez Gene: 3772325 Drosophila melanogaster
    • Entrez Gene: 3772509 Drosophila melanogaster
    • Entrez Gene: 3772519 Drosophila melanogaster
    • Entrez Gene: 3772666 Drosophila melanogaster
    • Entrez Gene: 3772708 Drosophila melanogaster
    • Entrez Gene: 41773 Drosophila melanogaster
    • Entrez Gene: 121504 Human
    • Entrez Gene: 554313 Human
    • Entrez Gene: 8294 Human
    • Entrez Gene: 8359 Human
    • Entrez Gene: 8360 Human
    • Entrez Gene: 8361 Human
    • Entrez Gene: 8362 Human
    • Entrez Gene: 8363 Human
    • Entrez Gene: 8364 Human
    • Entrez Gene: 8365 Human
    • Entrez Gene: 8366 Human
    • Entrez Gene: 8367 Human
    • Entrez Gene: 8368 Human
    • Entrez Gene: 8370 Human
    • Entrez Gene: 100041230 Mouse
    • Entrez Gene: 319155 Mouse
    • Entrez Gene: 319156 Mouse
    • Entrez Gene: 319157 Mouse
    • Entrez Gene: 319158 Mouse
    • Entrez Gene: 319159 Mouse
    • Entrez Gene: 319160 Mouse
    • Entrez Gene: 319161 Mouse
    • Entrez Gene: 320332 Mouse
    • Entrez Gene: 326619 Mouse
    • Entrez Gene: 326620 Mouse
    • Entrez Gene: 674678 Mouse
    • Entrez Gene: 69386 Mouse
    • Entrez Gene: 97122 Mouse
    • Entrez Gene: 64627 Rat
    • Omim: 142750 Human
    • Omim: 602822 Human
    • Omim: 602823 Human
    • Omim: 602824 Human
    • Omim: 602825 Human
    • Omim: 602826 Human
    • Omim: 602827 Human
    • Omim: 602828 Human
    • Omim: 602829 Human
    • Omim: 602830 Human
    • Omim: 602831 Human
    • Omim: 602833 Human
    • SwissProt: P84040 Drosophila melanogaster
    • SwissProt: P62805 Human
    • SwissProt: P62806 Mouse
    • SwissProt: P62804 Rat
    • Unigene: 21500 Drosophila melanogaster
    • Unigene: 29514 Drosophila melanogaster
    • Unigene: 29527 Drosophila melanogaster
    • Unigene: 30219 Drosophila melanogaster
    • Unigene: 30220 Drosophila melanogaster
    • Unigene: 30221 Drosophila melanogaster
    • Unigene: 30223 Drosophila melanogaster
    • Unigene: 30868 Drosophila melanogaster
    • Unigene: 30869 Drosophila melanogaster
    • Unigene: 30871 Drosophila melanogaster
    • Unigene: 30872 Drosophila melanogaster
    • Unigene: 30873 Drosophila melanogaster
    • Unigene: 30876 Drosophila melanogaster
    • Unigene: 33873 Drosophila melanogaster
    • Unigene: 5747 Drosophila melanogaster
    • Unigene: 143080 Human
    • Unigene: 247816 Human
    • Unigene: 248172 Human
    • Unigene: 248178 Human
    • Unigene: 248179 Human
    • Unigene: 278483 Human
    • Unigene: 46423 Human
    • Unigene: 528055 Human
    • Unigene: 533295 Human
    • Unigene: 55468 Human
    • Unigene: 591790 Human
    • Unigene: 655235 Human
    • Unigene: 662174 Human
    • Unigene: 706635 Human
    • Unigene: 718935 Human
    • Unigene: 144300 Mouse
    • Unigene: 14775 Mouse
    • Unigene: 158272 Mouse
    • Unigene: 227295 Mouse
    • Unigene: 228709 Mouse
    • Unigene: 246720 Mouse
    • Unigene: 255646 Mouse
    • Unigene: 260530 Mouse
    • Unigene: 261642 Mouse
    • Unigene: 261662 Mouse
    • Unigene: 261664 Mouse
    • Unigene: 377875 Mouse
    • Unigene: 442307 Mouse
    see all
  • Alternative names

    • dJ160A22.1 antibody
    • dJ160A22.2 antibody
    • dJ221C16.1 antibody
    • dJ221C16.9 antibody
    • FO108 antibody
    • H4 antibody
    • H4.k antibody
    • H4/a antibody
    • H4/b antibody
    • H4/c antibody
    • H4/d antibody
    • H4/e antibody
    • H4/g antibody
    • H4/h antibody
    • H4/I antibody
    • H4/j antibody
    • H4/k antibody
    • H4/m antibody
    • H4/n antibody
    • H4/p antibody
    • H4_HUMAN antibody
    • H4F2 antibody
    • H4F2iii antibody
    • H4F2iv antibody
    • H4FA antibody
    • H4FB antibody
    • H4FC antibody
    • H4FD antibody
    • H4FE antibody
    • H4FG antibody
    • H4FH antibody
    • H4FI antibody
    • H4FJ antibody
    • H4FK antibody
    • H4FM antibody
    • H4FN antibody
    • H4M antibody
    • HIST1H4A antibody
    • HIST1H4B antibody
    • HIST1H4C antibody
    • HIST1H4D antibody
    • HIST1H4E antibody
    • HIST1H4F antibody
    • HIST1H4H antibody
    • HIST1H4I antibody
    • HIST1H4J antibody
    • HIST1H4K antibody
    • HIST1H4L antibody
    • HIST2H4 antibody
    • HIST2H4A antibody
    • Hist4h4 antibody
    • Histone 1 H4a antibody
    • Histone 1 H4b antibody
    • Histone 1 H4c antibody
    • Histone 1 H4d antibody
    • Histone 1 H4e antibody
    • Histone 1 H4f antibody
    • Histone 1 H4h antibody
    • Histone 1 H4i antibody
    • Histone 1 H4j antibody
    • Histone 1 H4k antibody
    • Histone 1 H4l antibody
    • Histone 2 H4a antibody
    • histone 4 H4 antibody
    • Histone H4 antibody
    • MGC24116 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (di methyl K20) antibody (ab9052)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (di methyl K20) antibody (ab9052)

    ab9052 stained in HeLa cells. Cells were fixed with 4% paraformaldehyde (10 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% Triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab9052 at 0.5µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.

  • Immunoprecipitation - Anti-Histone H4 (di methyl K20) antibody (ab9052)
    Immunoprecipitation - Anti-Histone H4 (di methyl K20) antibody (ab9052)

    Histone H4 (di methyl K20) was immunoprecipitated using 0.5mg HeLa whole cell extract, 5µg of Rabbit polyclonal to Histone H4 (di methyl K20) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab9052.
    Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
    Band: 14kDa: Histone H4 (di methyl K20).

  • Western blot - Anti-Histone H4 (di methyl K20) antibody (ab9052)
    Western blot - Anti-Histone H4 (di methyl K20) antibody (ab9052)This image is courtesy of Steve Sanders, Tony Kouzarides lab, University of Cambridge
    All lanes : Anti-Histone H4 (di methyl K20) antibody (ab9052) at 1/2000 dilution

    Lane 1 : yH4 17-24 peptide
    Lane 2 : Unmodified yH4 17-24 peptide
    Lane 3 : Mono methyl K20 yH4 17-24 peptide
    Lane 4 : Di methyl K20 yH4 17-24 peptide
    Lane 5 : Tri methyl K20 yH4 17-24 peptide

    Blocking peptides at 1 µg/ml per lane.

    Performed under reducing conditions.
  • Western blot - Anti-Histone H4 (di methyl K20) antibody (ab9052)
    Western blot - Anti-Histone H4 (di methyl K20) antibody (ab9052)
    All lanes : Upper blot - Histone H4 antibody (gift of A. Verreault)

    Lower blot - Rabbit polyclonal to Histone H4 di methyl K20 (ab9052) at 1/2000

    Lane 1 : S.cerevisiae extract
    Lane 2 : S.pombe extract
    Lane 3 : S.pombe rH4
    Lane 4 : Drosophila rH4
    Lane 5 : Histones (Roche)


    Di methylation at K20 is seen in S. pombe and in calf thymus (Roche). S. cerevisiae lacks K20 (di) methylation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (di methyl K20) antibody (ab9052)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (di methyl K20) antibody (ab9052)
    IHC image of ab9052 staining in human pancreas adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9052, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (46)

Publishing research using ab9052? Please let us know so that we can cite the reference in this datasheet.

ab9052 has been referenced in 46 publications.

  • Michelena J  et al. Replicated chromatin curtails 53BP1 recruitment in BRCA1-proficient and BRCA1-deficient cells. Life Sci Alliance 4:N/A (2021). PubMed: 33811064
  • Xu X  et al. The epigenetic regulator LSH maintains fork protection and genomic stability via MacroH2A deposition and RAD51 filament formation. Nat Commun 12:3520 (2021). PubMed: 34112784
  • Tang M  et al. FOXK1 Participates in DNA Damage Response by Controlling 53BP1 Function. Cell Rep 32:108018 (2020). PubMed: 32783940
  • Shue YT  et al. Dynamic shifts in chromatin states differentially mark the proliferative basal cells and terminally differentiated cells of the developing epidermis. Epigenetics 15:932-948 (2020). PubMed: 32175801
  • Kim SA  et al. Crystal Structure of the LSD1/CoREST Histone Demethylase Bound to Its Nucleosome Substrate. Mol Cell 78:903-914.e4 (2020). PubMed: 32396821
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 11 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H4 (di methyl K20) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (CRC cells (Colon))
Permeabilization
Yes - Tween plus PBS
Specification
CRC cells (Colon)
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 12 2021

Immunohistochemistry (Frozen sections) abreview for Anti-Histone H4 (di methyl K20) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Colon cancer)
Permeabilization
No
Specification
Colon cancer
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 30 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H4 (di methyl K20) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - PBS-Tween
Specification
Colon cancer
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 05 2021

ChIP abreview for Anti-Histone H4 (di methyl K20) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
ChIP
Sample
Mouse Tissue lysate - nuclear (Hippocampus)
Negative control
Unspecific IgG
Specification
Hippocampus
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde in PBS
Positive control
2 concentration of the antibody: 1/500 and 1/250
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Ernest Palomer Vila

Verified customer

Submitted Aug 31 2018

Western blot abreview for Anti-Histone H4 (di methyl K20) antibody - ChIP Grade

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - nuclear (Nuclei prepared by tri-choloro acetic acid (TCA) p)
Gel Running Conditions
Reduced Denaturing (15% BIS-TRIS gel)
Loading amount
10 µg
Specification
Nuclei prepared by tri-choloro acetic acid (TCA) p
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 23 2018

Western blot abreview for Anti-Histone H4 (di methyl K20) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
African green monkey Cell lysate - whole cell (Cos7)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
Cos7
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 23 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H4 (di methyl K20) antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Caenorhabditis elegans Cell (Gut cell & embryos)
Specification
Gut cell & embryos
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 10 2015

Western blot abreview for Anti-Histone H4 (di methyl K20) antibody - ChIP Grade

Inconclusive
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - other (HeLa, mouse ES)
Gel Running Conditions
Reduced Denaturing (4-12 % SDS PAGE)
Loading amount
2 µg
Specification
HeLa, mouse ES
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Submitted Aug 04 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H4 (di methyl K20) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Na-citrate pH6
Sample
Mouse Tissue sections (Liver, P5)
Specification
Liver, P5
Permeabilization
Yes - Triton 0,05%
Fixative
Paraformaldehyde
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Oct 07 2014

Western blot abreview for Anti-Histone H4 (di methyl K20) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (multiple myeloma cells)
Loading amount
50 µg
Specification
multiple myeloma cells
Gel Running Conditions
Reduced Denaturing (10-20% tris glycine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 21 2012

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