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Methylation of histone H4 at lysine 20 is catalyzed by SET9 and SUV4.20h among other methyl transferases in mammalian cells. H4 (di-methyl K20) has been described as repressive chromatin domain and involved in DNA damage response.
Histone H4 (di-methyl K20) Quantification Kit (Fluorometric) (ab115096) enables the user to measure global di-methylation of histone H4K20. The global H4K20 di-methylation can also be changed by inhibiton or activation of HMTs, making quantitative detection of global di-methyl histone H4K20 a useful tool for better understanding epigenetic regulation of gene activation/repression. ab115096 is suitable for use with a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
|Components||Identifier||48 tests||96 tests|
|10X Wash Buffer||1 x 10ml||1 x 20ml|
|8-Well Sample Strips (with Frame)||4 units||9 units|
|8-Well Standard Control Strips||Green Ringed||2 units||3 units|
|Antibody Buffer||1 x 6ml||1 x 12ml|
|Detecting Antibody, 1 mg/mL||1 x 5µl||1 x 10µl|
|Fluoro Developer||1 x 12µl||1 x 24µl|
|Fluoro Dilution||1 x 4ml||1 x 8ml|
|Fluoro Enhancer||1 x 12µl||1 x 24µl|
|Signal Enhancer||1 x 120µl||1 x 240µl|
|Signal Report Solution||1 x 5µl||1 x 10µl|
|Standard Control, 100 µg/mL||1 x 10µl||1 x 20µl|
ab115096 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"