Product nameAnti-Histone H4 (di methyl K79) antibody
See all Histone H4 primary antibodies
DescriptionRabbit polyclonal to Histone H4 (di methyl K79)
SpecificitySpecific to Histone H4 K79 Di-methyl. In blocking experiments, is not blocked by H3 K79 peptides.
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, ICCmore details
Species reactivityReacts with: Cow, Human
Does not react with: Saccharomyces cerevisiae
Synthetic peptide within Human Histone H4 aa 50 to the C-terminus (di methyl K79) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
(Peptide available as
- Calf Thymus Histone Preparation; Hela whole cell extract
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab2885 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 14 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similaritiesBelongs to the histone H4 family.
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
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All lanes : Anti-Histone H4 (di methyl K79) antibody (ab2885) at 1/500 dilution
Lane 1 : Calf Thymus histone prep
Lane 2 : Calf Thymus histone prep with Human Histone H3 (mono methyl K79) peptide (ab4555) at 0.1 µg
Lane 3 : Calf Thymus histone prep with
Human Histone H3 (di methyl K79) peptide (ab4556) at 0.1 µg
Lane 4 : Calf Thymus histone prep with
Human Histone H3 (tri methyl K79) peptide (ab4557) at 0.1 µg
Lane 5 : Calf Thymus histone prep with Human Histone H4 (di methyl K79) peptide (ab4560) at 0.1 µg
Lane 6 : Calf Thymus histone prep with
Human Histone H3 (di methyl K27) peptide (ab1781) at 0.1 µg
Lysates/proteins at 1 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
Rabbit polyclonal to Histone H4 Di Methyl K79 at 1/500 on Calf Thymus histone prep (1 ug per lane).
Lane 1: ab2885 1/500
Lane 2: ab2885 1/500 H3 Mono Me K79 peptide (ab4555), 0.1 ug
Lane 3: ab2885 1/500 H3 Di Me K79 peptide (ab4556), 0.1 ug
Lane 4: ab2885 1/500 H3 Tri Me K79 peptide (ab4557), 0.1 ug
Lane 5: ab2885 1/500 H4 Di Me K79 peptide (ab4560), 0.1 ug
Lane 6: ab2885 1/500 H3 Di Me K27 peptide (ab1781), 0.1 ug
Secondary antibody: Goat anti-rabbit IgG (HRP) ab 1/2000 (ab6721)
SKN cells were stained with ab2885 (green) at a dilution of 1/200. The cells were fixed in paraformaldehyde for 10 minutes prior to incubation with ab2885. The DNA was stained with DAPI (blue). 100x magnification.
ab2885 at 1/2000 used in Western blot analysis of MCF7 cells (human breast cancer cells).
Lane 1: MCF7 cell lysate control
Lane 2: MCF7 cell lysate (cells treated with 50µm Troglitazone for 48 hours)
Lane 3: MCF7 cells lysate (cells treated with 1µm Trichostatin for 48 hours)
Troglitazone and trichostatin increase dimethyl H4 (K79) and act as anti-proliferatives in human breast cancer. The blot was stripped and reprobed with an alpha tubilin antibody to show loading equivalence.
IHC image of Histone H4 (di methyl K79) staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2885, 5µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
This product has been referenced in:
- Zhang K et al. Comparative analysis of histone H3 and H4 post-translational modifications of esophageal squamous cell carcinoma with different invasive capabilities. J Proteomics 112C:180-189 (2014). Read more (PubMed: 25234497) »