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Synthetic peptide within Human Histone H4 aa 50 to the C-terminus (di methyl K79) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
(Peptide available as
Our Abpromise guarantee covers the use of ab2885 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 14 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Rabbit polyclonal to Histone H4 Di Methyl K79 at 1/500 on Calf Thymus histone prep (1 ug per lane).
Lane 1: ab2885 1/500
Lane 2: ab2885 1/500 H3 Mono Me K79 peptide (ab4555), 0.1 ug
Lane 3: ab2885 1/500 H3 Di Me K79 peptide (ab4556), 0.1 ug
Lane 4: ab2885 1/500 H3 Tri Me K79 peptide (ab4557), 0.1 ug
Lane 5: ab2885 1/500 H4 Di Me K79 peptide (ab4560), 0.1 ug
Lane 6: ab2885 1/500 H3 Di Me K27 peptide (ab1781), 0.1 ug
Secondary antibody: Goat anti-rabbit IgG (HRP) ab 1/2000 (ab6721)
SKN cells were stained with ab2885 (green) at a dilution of 1/200. The cells were fixed in paraformaldehyde for 10 minutes prior to incubation with ab2885. The DNA was stained with DAPI (blue). 100x magnification.
ab2885 at 1/2000 used in Western blot analysis of MCF7 cells (human breast cancer cells).
Lane 1: MCF7 cell lysate control
Lane 2: MCF7 cell lysate (cells treated with 50µm Troglitazone for 48 hours)
Lane 3: MCF7 cells lysate (cells treated with 1µm Trichostatin for 48 hours)
Troglitazone and trichostatin increase dimethyl H4 (K79) and act as anti-proliferatives in human breast cancer. The blot was stripped and reprobed with an alpha tubilin antibody to show loading equivalence.
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