Key features and details
- Rabbit polyclonal to Histone H4 (di methyl R3)
- Suitable for: WB, IHC-P, Dot blot, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Histone H4 (di methyl R3) antibody
See all Histone H4 primary antibodies
DescriptionRabbit polyclonal to Histone H4 (di methyl R3)
Tested applicationsSuitable for: WB, IHC-P, Dot blot, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: a wide range of other species
Synthetic peptide corresponding to Human Histone H4 (N terminal) (di methyl R3).
Database link: P62805
- WB: HeLa cell lysate; IHC-P: Human stomach, rat liver and mouse liver tissues. ICC/ IF: 293T cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab194696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 11 kDa.|
|IHC-P||1/50 - 1/200.|
|Dot blot||Use at an assay dependent concentration.|
|ICC/IF||1/50 - 1/200.|
FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similaritiesBelongs to the histone H4 family.
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
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Paraffin-embedded human stomach tissue stained for Histone H4 (di methyl R3) using ab194696 at 1/100 dilution in immunohistochemical analysis.
Immunofluorescence staining of 293T cells stained for Histone H4 (di methyl R3) with ab194696. Nuclei are labeled with DAPI (Blue).
All lanes : Anti-Histone H4 (di methyl R3) antibody (ab194696)
Lane 1 : HeLa cell lysate
Lane 2 : H4 protein
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Predicted band size: 11 kDa
Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of methylation peptides using ab194696.
Paraffin-embedded mouse liver tissue stained for Histone H4 (di methyl R3) using ab194696 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded rat liver tissue stained for Histone H4 (di methyl R3) using ab194696 at 1/100 dilution in immunohistochemical analysis.
ab194696 has been referenced in 1 publication.
- Chen K et al. Neurodegenerative Disease Proteinopathies Are Connected to Distinct Histone Post-translational Modification Landscapes. ACS Chem Neurosci 9:838-848 (2018). PubMed: 29243911