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Histone H4 (di-methyl R3) Quantification Kit (Colorimetric) (ab156914) is suitable for specifically measuring global histone H4 arginine 3 di-methylation from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including cultured cells and fresh tissues.
Arginine histone methylation is one of the many important epigenetic marks, and is essential for the regulation of multiple cellular processes. Arginine methylation of histones H3 (Arg2, 8, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs). There are 9 types of PRMTs found in humans but only 7 members are reported to methylate histones. They can mediate mono or dimethylation of arginine residues. These enzymes use S-adenosyl-methionine (SAM) as a methyl donor and transfer it to the guanidinium side chain of arginine. Based on the position of methyl group addition, the PRMTs can be classified into type I (CARM1, PRMT1, PRMT2, PRMT3, PRMT6, and PRMT8) and type II (PRMT5 and PRMT7).
Symmetric di-methylation of histone H4 arg3 (H4R3) are catalyzed by type II PRMTs, which are found to be strongly implicated in diseases like cancer. For example, PRMT5 plays a role in the repression ofcertain tumor suppressor genes such as RB tumor suppressors while PRMT7 overexpression is observed in breast cancer. The global H4R3 di-methylation can be changed by inhibition or activation of type II PRMTs. Therefore, quantitative detection of global symmetric di-methyl histone H4R3 would provide useful information for better understanding epigenetic regulation of gene activation and silencing, as well as for developing PRMT-targeted drugs.
|Components||48 tests||96 tests|
|1000X Capture Antibody||1 x 5µl||1 x 10µl|
|10X Wash Buffer||1 x 14ml||1 x 28ml|
|2000X Detection Antibody||1 x 6µl||1 x 12µl|
|8-Well Assay Strips (with Frame)||1 x 6 units||1 x 12 units|
|Adhesive Covering Film||1 unit||1 unit|
|Blocking Buffer||1 x 10ml||1 x 20ml|
|Developer Solution||1 x 5ml||1 x 10ml|
|Enhancer Solution||1 x 6µl||1 x 12µl|
|H4R3me2 Control, 50 µg/mL||1 x 10µl||1 x 20µl|
|Histone Buffer||1 x 4ml||1 x 8ml|
|Stop Solution||1 x 5ml||1 x 10ml|
Illustrated standard curve generated with H4R3me2 Control.
Histone extracts were prepared from MDA-231 cells using Histone Extraction Kit (ab113476) and the amount of H4R3me2 was measured using Abcam's Histone H3 (di-methyl R4) Quantification Kit (Colorimetric) (ab156914).
ab156914 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"