Anti-Histone H4 (formyl K12) antibody (ab177068)

Overview

  • Product name
    Anti-Histone H4 (formyl K12) antibody
    See all Histone H4 primary antibodies
  • Description
    Rabbit polyclonal to Histone H4 (formyl K12)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human
  • Immunogen

    Synthetic peptide corresponding to Human Histone H4 aa 1-100 (formyl K12) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P62805

  • Positive control
    • This antibody gave a positive signal in HeLa and NIH3T3 whole cell lysates as well as Calf Thymus Histone (CTH) and HeLa Nuclear Prep (0.5% Triton X-100 insoluble fraction) ICC/IF – HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab177068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

Images

  • ab177068 staining Histone H4 (formyl K12) in HeLa cells. The cells were fixed with 100% methanol (5min) and then permeabilised using 0.1% PBS-Triton. Cells were then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab177068 at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150081) at 1/1000 dilution (shown in green). AlexaFluor®594 Mouse monoclonal [DM1A] to alpha Tubulin (ab195889) - Microtubule Marker was used at a 1/200 dilution and incubated for 1h with the cells, to label Microtubules (shown in Red). The nuclear counter stain is DAPI (blue), which was added to the secondary antibody mixture.

  • All lanes : Anti-Histone H4 (formyl K12) antibody (ab177068) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa Nuclear Prep (0.5% Triton X-100 insoluble fraction)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) at 1/10000 dilution

    Predicted band size: 11 kDa
    Observed band size: 14 kDa
    why is the actual band size different from the predicted?



    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab177068 overnight at 4°C. Antibody binding was detected using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  • All lanes : Anti-Histone H4 (formyl K12) antibody (ab177068) at 1 µg/ml

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
    Lane 2 : Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) at 1/10000 dilution

    Predicted band size: 11 kDa
    Observed band size: 14 kDa why is the actual band size different from the predicted?
    Additional bands at: 50 kDa (possible non-specific binding)



    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab177068 overnight at 4°C. Antibody binding was detected using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

References

ab177068 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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