Overview

  • Product name
    Histone H4 Modification Multiplex Assay Kit (Colorimetric)
    See all Histone H4 kits
  • Detection method
    Colorimetric
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay type
    Quantitative
  • Sensitivity
    0.5 ng/well
  • Range
    20 ng/well - 500 ng/well
  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Plants, Fungi
  • Product overview

    Abcam's Histone H4 Modification Multiplex Assay Kit (Colorimetric) (ab185914) is a complete set of optimized reagents to detect and quantify nearly all histone H4 modifications (10 different types) simultaneously in a simple, ELISA-like format with use of a standard microplate reader.

     ab185914 is designed for measuring multiple histone H4 modifications simultaneously. In an assay with this kit, each histone H4 modified at specific sites will be captured by an antibody that is coated on the strip wells and specifically targets the appropriate histone H4 modification pattern. The captured histone modified at specific sites will be detected with a detection antibody, followed by a color development reagent. The ratio of modified histone is proportional to the intensity of absorbance measured by a microplate reader at a wavelength of 450 nm.

    Starting Materials
    Input materials can be histone extracts or purified histone H4 proteins obtained from human, mouse, rat, as well as a broad range of species including most plants, fungi, and bacteria, based on high sequence homology of histone H4. The amount of histone extracts for each assay can be 20 ng to 500 ng with an optimal range of 50 to 100 ng depending on the purity of histone extracts. The amount of purified histone H4 proteins for each assay can be 1 ng to 25 ng with an optimal range of 4 to 5 ng.

     

  • Platform
    Microplate reader

Properties

Associated products

Images

  • Histone extracts were prepared from MCF-7 and MDA-231 cells using the Histone Extraction Kit (ab113476). 10 histone H4 modifications were measured using the Histone H4 Modification Multiplex Assay Kit (Colorimetric) (ab185914). 100 ng of total histone proteins per well were used.

Protocols

References

ab185914 has not yet been referenced specifically in any publications.

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