Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 2 hr 30 min
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 0.5 ng/well
Product nameHistone H4 Modification Multiplex Assay Kit (Colorimetric)
See all Histone H4 kits
Sample typeTissue, Adherent cells, Suspension cells
Range20 ng/well - 500 ng/well
Assay time2h 30m
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Plants, Fungi
Abcam's Histone H4 Modification Multiplex Assay Kit (Colorimetric) (ab185914) is a complete set of optimized reagents to detect and quantify nearly all histone H4 modifications (10 different types) simultaneously in a simple, ELISA-like format with use of a standard microplate reader.
ab185914 is designed for measuring multiple histone H4 modifications simultaneously. In an assay with this kit, each histone H4 modified at specific sites will be captured by an antibody that is coated on the strip wells and specifically targets the appropriate histone H4 modification pattern. The captured histone modified at specific sites will be detected with a detection antibody, followed by a color development reagent. The ratio of modified histone is proportional to the intensity of absorbance measured by a microplate reader at a wavelength of 450 nm.
Input materials can be histone extracts or purified histone H4 proteins obtained from human, mouse, rat, as well as a broad range of species including most plants, fungi, and bacteria, based on high sequence homology of histone H4. The amount of histone extracts for each assay can be 20 ng to 500 ng with an optimal range of 50 to 100 ng depending on the purity of histone extracts. The amount of purified histone H4 proteins for each assay can be 1 ng to 25 ng with an optimal range of 4 to 5 ng.
Storage instructionsPlease refer to protocols.
Components 96 tests 10X Wash Buffer 1 x 28ml 8-Well Strips 2 units 96-Well Strip Plate (with Frame) 1 unit Adhesive Covering Film 1 unit Antibody Buffer 1 x 8ml Assay Control Protein 1 x 20µl Detection Antibody 1 x 12µl Developer Solution 1 x 12ml Stop Solution 1 x 12ml
RelevanceCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Histone extracts were prepared from MCF-7 and MDA-231 cells using the Histone Extraction Kit (ab113476). 10 histone H4 modifications were measured using the Histone H4 Modification Multiplex Assay Kit (Colorimetric) (ab185914). 100 ng of total histone proteins per well were used.
ab185914 has not yet been referenced specifically in any publications.