Product nameAnti-Histone H4 (mono methyl K20) antibody
See all Histone H4 primary antibodies
DescriptionRabbit polyclonal to Histone H4 (mono methyl K20)
Tested applicationsSuitable for: WB, Dot blot, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Xenopus laevis
Synthetic peptide within Human Histone H4 (N terminal) (mono methyl K20). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: P62805
- WB: HeLa whole cell and nuclear lysates. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 11 kDa.|
|Dot blot||Use at an assay dependent concentration.|
|ICC/IF||1/100 - 1/1000.|
FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similaritiesBelongs to the histone H4 family.
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- dJ160A22.1 antibody
- dJ160A22.2 antibody
- dJ221C16.1 antibody
All lanes : Anti-Histone H4 (mono methyl K20) antibody (ab227947) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 11 kDa
15% SDS-PAGE gel.
Dot blot analysis of ab227947 with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with ab227947 at 1/3000 dilution.
Lane 1: Peptide samples of H4K20.
Lane 2: Peptide samples of H4K20me1.
Lane 3: Peptide samples of H4K20me2.
Lane 4: Peptide samples of H4K20me3.
4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H4 (mono methyl K20) (green) using ab227947 at 1/500 dilution in ICC/IF.
Counter stain: alpha Tubulin, a cytoskeleton marker, stained by an anti-alpha Tubulin antibody at 1/500 dilution (red). Nuclear counterstain: Hoechst 33342 (blue).
Panel A: Histone H4 (methyl K20) protein labeled with ab227947. Panel B: alpha Tubulin labeled with anti-alpha Tubulin antibody. Panel C: Merge of all stainings.
ab227947 has not yet been referenced specifically in any publications.