Overview

  • Product name

    Anti-Histone H4 (mono methyl K20) antibody
    See all Histone H4 primary antibodies
  • Description

    Rabbit polyclonal to Histone H4 (mono methyl K20)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Dot blot, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Xenopus laevis
  • Immunogen

    Synthetic peptide within Human Histone H4 (N terminal) (mono methyl K20). The exact sequence is proprietary. (Carrier-protein conjugated).
    Database link: P62805

  • Positive control

    • WB: HeLa whole cell and nuclear lysates. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 11 kDa.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-Histone H4 (mono methyl K20) antibody (ab227947) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 11 kDa



    15% SDS-PAGE gel.

  • Dot blot analysis of ab227947 with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with ab227947 at 1/3000 dilution.

    Lane 1: Peptide samples of H4K20.
    Lane 2: Peptide samples of H4K20me1.
    Lane 3: Peptide samples of H4K20me2.
    Lane 4: Peptide samples of H4K20me3.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H4 (mono methyl K20) (green) using ab227947 at 1/500 dilution in ICC/IF.

    Counter stain: alpha Tubulin, a cytoskeleton marker, stained by an anti-alpha Tubulin antibody at 1/500 dilution (red). Nuclear counterstain: Hoechst 33342 (blue).

    Panel A: Histone H4 (methyl K20) protein labeled with ab227947. Panel B: alpha Tubulin labeled with anti-alpha Tubulin antibody. Panel C: Merge of all stainings.

References

ab227947 has not yet been referenced specifically in any publications.

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