Recombinant
RabMAb

Recombinant Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (ab177828)

Overview

  • Product name

    Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)]
    See all Histone H4 primary antibodies
  • Description

    Rabbit monoclonal [EPR17921(2)] to Histone H4 (mono methyl K77)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: PepArr, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Histone H4 aa 50 to the C-terminus (mono methyl K77). The exact sequence is proprietary.
    Database link: P62805

  • Positive control

    • WB: HeLa and NIH/3T3 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17921(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
PepArr Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).

Target

Images

  • Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (ab177828) at 1/1000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% BSA/TBST.

  • Anti-Histone H4 (mono methyl K77) antibody [EPR17921(2)] (ab177828) at 1/1000 dilution + NIH/3T3 (Mouse embyro fibroblast cells) cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% BSA/TBST.

  • ab177828 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.

References

ab177828 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab177828.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up