Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19561] to Histone H4 (mono methyl R78)
- Suitable for: Dot blot, ICC/IF, Flow Cyt, WB
- Reacts with: Mouse, Human
Product nameAnti-Histone H4 (mono methyl R78) antibody [EPR19561]
See all Histone H4 primary antibodies
DescriptionRabbit monoclonal [EPR19561] to Histone H4 (mono methyl R78)
Tested applicationsSuitable for: Dot blot, ICC/IF, Flow Cyt, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human Histone H4 aa 50 to the C-terminus (mono methyl R78). The exact sequence is proprietary.
Database link: P62805
- WB: HeLa and NIH/3T3 whole cell lysates. ICC/IF: HeLa cells. Flow Cyt: HeLa cells. Dot blot: Histone H4 (mono methyl R78) peptide.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab202036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).|
FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similaritiesBelongs to the histone H4 family.
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- dJ160A22.1 antibody
- dJ160A22.2 antibody
- dJ221C16.1 antibody
All lanes : Anti-Histone H4 (mono methyl R78) antibody [EPR19561] (ab202036) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma ) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 3 minutes
Blocking/Dilution buffer: 5% NFDM/TBST.
Dot blot analysis of Histone H4 (mono methyl R78) labeled with ab202036 at 1/1000 dilution.
Lane 1: Histone H4 (mono methyl R78) peptide.
Lane 2: Histone H4 (asymmetric di methyl R78) peptide.
Lane 3: Histone H4 (symmetric di methyl R78) peptide.
Lane 4: Histone H4 unmodified peptide.
Lane 5: Histone H4 (mono methyl K77) peptide.
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Exposure time: 3 minutes.
Blocking and dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 2% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H4 (mono methyl R78) with ab202036 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on Hela cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Histone H4 (mono methyl R78) with ab202036 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab202036 has not yet been referenced specifically in any publications.