Recombinant
RabMAb

Recombinant Anti-Histone H4 (mono methyl R78) antibody [EPR19561] (ab202036)

Overview

  • Product name

    Anti-Histone H4 (mono methyl R78) antibody [EPR19561]
    See all Histone H4 primary antibodies
  • Description

    Rabbit monoclonal [EPR19561] to Histone H4 (mono methyl R78)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Dot blot, ICC/IF, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Histone H4 aa 50 to the C-terminus (mono methyl R78). The exact sequence is proprietary.
    Database link: P62805

  • Positive control

    • WB: HeLa and NIH/3T3 whole cell lysates. ICC/IF: HeLa cells. Flow Cyt: HeLa cells. Dot blot: Histone H4 (mono methyl R78) peptide.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19561
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab202036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/1000.
ICC/IF 1/250.
Flow Cyt 1/60.
WB 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).

Target

Images

  • All lanes : Anti-Histone H4 (mono methyl R78) antibody [EPR19561] (ab202036) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma ) whole cell lysate
    Lane 2 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Dot blot analysis of Histone H4 (mono methyl R78) labeled with ab202036 at 1/1000 dilution.

    Lane 1: Histone H4 (mono methyl R78) peptide.

    Lane 2: Histone H4 (asymmetric di methyl R78) peptide.

    Lane 3: Histone H4 (symmetric di methyl R78) peptide.

    Lane 4: Histone H4 unmodified peptide.

    Lane 5: Histone H4 (mono methyl K77) peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

     

    Exposure time: 3 minutes.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunofluorescent analysis of 2% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H4 (mono methyl R78) with ab202036 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on Hela cells.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Histone H4 (mono methyl R78) with ab202036 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

References

ab202036 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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