Product nameHistone H4 (pan-methyl K20) Quantification Kit (Colorimetric)
See all Histone H4 (K20 methylation) kits
Sample typeTissue, Adherent cells, Suspension cells
Range20 ng/well - 2000 ng/well
Assay time2h 30m
Species reactivityReacts with: Mouse, Human
Predicted to work with: Mammals
Methylation of histone H4 at lysine 20 is catalyzed by SET9 and SUV4.20h among other methyl transferases in mammalian cells. H4 (mono-methyl K20) is involved in the maintenance of proper higher order structure of DNA and is consequently essential for chromosome condensation, as well as functioning in gene silencing. H4 (di-methyl K20) has been described as another repressive chromatin domain and is involved in the DNA damage response. H4 (tri-methyl K20) acts as a passive feature or structure determinant for chromatin degradation and release, as well as being an epigenetic marker of early apoptosis. H4 (tri-methyl K20) is also considered as a common hallmark of human cancer.
Histone H4 (pan-methyl K20) Quantification Kit (Colorimetric) (ab115099) enables the user to measure global mono-, di- and tri-methylation of histone H4K20. The global H4K20 pan-methylation can also be changed by inhibiton or activation of HMTs, making quantitative detection of global pan-methyl histone H4K20 a useful tool for better understanding epigenetic regulation of gene activation/repression. ab115099 is suitable for use with a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
Storage instructionsPlease refer to protocols.
Components Identifier 96 tests 10X Wash Buffer 1 x 20ml 8-Well Sample Strips (with Frame) 1 x 9 units 8-Well Standard Control Strips Green Ringed 1 x 3 units Antibody Buffer 1 x 12ml Color Developer 1 x 10ml Detecting Antibody, 1 mg/mL 1 x 10µl Signal Report Solution 1 x 10µl Signal Report Solution 1 x 240µl Standard Control, 100 µg/mL 1 x 20µl Stop Solution 1 x 6ml
- Histone H4 (mono-methyl K20) Quantification Kit (Colorimetric) (ab115093)
- Histone H4 (mono-methyl K20) Quantification Kit (Fluorometric) (ab115094)
- Histone H4 (di-methyl K20) Quantification Kit (Colorimetric) (ab115095)
- Histone H4 (di-methyl K20) Quantification Kit (Fluorometric) (ab115096)
- Histone H4 (tri-methyl K20) Quantification Kit (Colorimetric) (ab115097)
- Histone H4 (tri-methyl K20) Quantification Kit (Fluorometric) (ab115098)
- Histone H4 (pan-methyl K20) Quantification Kit (Fluorometric) (ab115100)
ab115099 has not yet been referenced specifically in any publications.