Product nameAnti-Histone H4 (phospho S47) antibody
See all Histone H4 primary antibodies
DescriptionRabbit polyclonal to Histone H4 (phospho S47)
Specificityab61061 detects endogenous levels of Histone H4 only when phosphorylated at serine 47 (Human: Ser47; Mouse: Ser47).
Tested applicationsSuitable for: ICC/IF, ELISA, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- HuvEc cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab61061 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab61061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/1000.|
FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similaritiesBelongs to the histone H4 family.
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- dJ160A22.1 antibody
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Immunofluorescence analysis of HuvEc cells, using Histone H4 (phospho S47) antibody (ab61061) at 1/500 - 1/1000 dilution, in the presence (right panel) or absence (left panel) of phosphopeptide.
All lanes : Anti-Histone H4 (phospho S47) antibody (ab61061) at 1/1000 dilution
Lane 1 : Lystaes were prepared from HeLa cells expressing rat wild type histone H4
Lane 2 : Lystaes were prepared from Hela cells expressing rat S47A mutated histone H4
Lysates/proteins at 5 µg per lane.
All lanes : HRP-conjugated goat polyclonal to rabbit IgG at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 13 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
ab61061 has not yet been referenced specifically in any publications.