Western blot abreview for Anti-Histone H4 (phospho S47) antibody

Poor
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (liver)
Loading amount
1 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C

Secondary antibody

Name
Non-Abcam antibody was used: anto H4S47p
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Detection

Detection method
ecl
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 20 kDa

Additional data

Additional Notes
This antibody recognize a 20kd band which is not H4S47p
Abcam response
Thank you for your honest and valuable feedback. Abcam has yet to test this antibody in Western Blot. However, to improve your results, I would recommend increasing the loading amount to 20-30ug protein, and changing primary incubation time/temperature to overnight at 4C.

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Verified customer

Submitted Apr 17 2009

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