Overview

  • Product name

    Anti-Histone H4 (propionyl K16) antibody
  • Description

    Rabbit polyclonal to Histone H4 (propionyl K16)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Immunogen

    Synthetic peptide within Human Histone H4 (propionyl K16). The exact sequence is proprietary.
    Database link: P62805

  • Positive control

    • WB: HeLa, A549, HEK-293 and K562, treated with 10 mM sodium propionate for 4 hours, whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab240612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 11 kDa.

Target

  • Relevance

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involvement in disease Chromosomal aberrations involving HISTONE H4 is a cause of B-cell non-Hodgkin lymphomas (B-cell NHL). Translocation t(3;6)(q27;p21), with BCL6. Post-translational modification Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin. Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation. Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing. Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4. Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me). Sumoylated, which is associated with transcriptional repression. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
  • Cellular localization

    Nuclear
  • Alternative names

    • H4/A antibody
    • H4/B antibody
    • H4/G antibody
    • H4/I antibody
    • H4/J antibody
    • h4f2 antibody
    • H4FA antibody
    • H4FB antibody
    • H4FG antibody
    • H4FI antibody
    • H4FJ antibody
    • h4fn antibody
    • HIST1H4A antibody
    • HIST1H4B antibody
    • HIST1H4C antibody
    • HIST1H4D antibody
    • HIST1H4E antibody
    • HIST1H4F antibody
    • HIST1H4H antibody
    • HIST1H4I antibody
    • HIST1H4J antibody
    • HIST1H4K antibody
    • HIST1H4L antibody
    • Hist2H4 antibody
    • HIST2H4A antibody
    • HIST4H4 antibody
    see all

Images

  • All lanes : Anti-Histone H4 (propionyl K16) antibody (ab240612) at 1/100 dilution

    Lane 1 : Hela (human epithelial cell line from cervix adenocarcinoma) whole cell lysate, treated (+) with 10mM Sodium propionate for 4hr
    Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate, treated (+) with 10mM Sodium propionate for 4hr
    Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 10mM Sodium propionate for 4hr
    Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate, treated (+) with 10mM Sodium propionate for 4hr
    Lane 5 : Hela whole cell lysate, untreated (-)
    Lane 6 : A549 whole cell lysate, untreated (-)
    Lane 7 : HEK-293 whole cell lysate, untreated (-)
    Lane 8 : K562 whole cell lysate, untreated (-)

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 11 kDa

References

ab240612 has not yet been referenced specifically in any publications.

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