Key features and details
- Rabbit polyclonal to Histone H4 (propionyl K16)
- Suitable for: WB
- Isotype: IgG
Product nameAnti-Histone H4 (propionyl K16) antibody
DescriptionRabbit polyclonal to Histone H4 (propionyl K16)
Tested applicationsSuitable for: WBmore details
Synthetic peptide within Human Histone H4 (propionyl K16). The exact sequence is proprietary.
Database link: P62805
- WB: HeLa, A549, HEK-293 and K562, treated with 10 mM sodium propionate for 4 hours, whole cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab240612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Predicted molecular weight: 11 kDa.|
RelevanceCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involvement in disease Chromosomal aberrations involving HISTONE H4 is a cause of B-cell non-Hodgkin lymphomas (B-cell NHL). Translocation t(3;6)(q27;p21), with BCL6. Post-translational modification Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin. Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation. Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing. Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4. Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me). Sumoylated, which is associated with transcriptional repression. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
- H4/A antibody
- H4/B antibody
- H4/G antibody
All lanes : Anti-Histone H4 (propionyl K16) antibody (ab240612) at 1/100 dilution
Lane 1 : Hela (human epithelial cell line from cervix adenocarcinoma) whole cell lysate, treated (+) with 10mM Sodium propionate for 4hr
Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate, treated (+) with 10mM Sodium propionate for 4hr
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 10mM Sodium propionate for 4hr
Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate, treated (+) with 10mM Sodium propionate for 4hr
Lane 5 : Hela whole cell lysate, untreated (-)
Lane 6 : A549 whole cell lysate, untreated (-)
Lane 7 : HEK-293 whole cell lysate, untreated (-)
Lane 8 : K562 whole cell lysate, untreated (-)
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 11 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240612 has not yet been referenced specifically in any publications.