Key features and details
- Rabbit polyclonal to Histone H4 (propionyl K5)
- Suitable for: IP, WB, ICC
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Histone H4 (propionyl K5) antibody
See all Histone H4 primary antibodies
DescriptionRabbit polyclonal to Histone H4 (propionyl K5)
Tested applicationsSuitable for: IP, WB, ICCmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Histone H4 (propionyl K5).
Database link: P62805
- WB: HeLa, Jurkat and HEK-293T, treated with 10mM sodium propionate for 4hr, whole cell lysates. ICC: HeLa cells (treated with 10mM sodium propionate for 4hr). IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityImmunogen affinity purified
Alkaline Phosphatase detection kit
Our Abpromise guarantee covers the use of ab241252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/200 - 1/2000.|
|WB||1/500 - 1/2000.|
|ICC||1/20 - 1/200.|
FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similaritiesBelongs to the histone H4 family.
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
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All lanes : Anti-Histone H4 (propionyl K5) antibody (ab241252) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate, treated (+) with 10mM sodium propionate for 4hr
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate, treated (+) with 10mM sodium propionate for 4hr
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 10mM sodium propionate for 4hr
Lane 4 : HeLa whole cell lysate, untreated (-)
Lane 5 : Jurkat whole cell lysate, untreated (-)
Lane 6 : HEK-293 whole cell lysate, untreated (-)
All lanes : Goat polyclonal to rabbit IgG at 1/40000 dilution
Histone H4 (propionyl K5) was immunoprecipitated from 500 µg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab241252 at 1/200 dilution.
Lane 1: Rabbit control IgG IP in HeLa whole cell lysate.
Lane 2: ab241252 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 µg (Input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with 10mM sodium propionate for 4hr) stained for Histone H4 (propionyl K5) using ab241252 at 1/100 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Chromatin Immunoprecipitation HeLa (Human epithelial cell line from cervix adenocarcinoma) (4 x 106, treated with 10mM sodium propionate for 4h) were treated with micrococcal nuclease, sonicated, and immunoprecipitated with 5 μg ab241252 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241252 has not yet been referenced specifically in any publications.