Overview

  • Product name

    Histone H4 Total Acetylation Assay Kit (Fluorometric)
    See all Histone H4 acetylation kits
  • Detection method

    Fluorescent
  • Sample type

    Tissue, Adherent cells, Suspension cells
  • Sensitivity

    > 0.4 ng/well
  • Range

    5 ng/well - 2000 ng/well
  • Assay time

    2h 30m
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Mammals
  • Product overview

    Acetylation of histones such histone H4 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H4 actylation. Reversible acetylation of nucleosomal histone H4 is believed to correlate with potential transcriptional activity of eukaryotic chromatin domains. The reversible lysine acetylation of histone H4 may play a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication and repair, nuclear import and neuronal repression.

    ab131562 enables the user to fluorometrically detect and quantify if histone H4 is acetylated. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. ab131562 is suitable for specifically measuring total histone H4 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.

  • Platform

    Microplate reader

Properties

Protocols

References

This product has been referenced in:

  • Pestana JL  et al. Non-lethal heat shock increases tolerance to metal exposure in brine shrimp. Environ Res 151:663-670 (2016). Read more (PubMed: 27619211) »
  • Norouzitallab P  et al. Probing the phenomenon of trained immunity in invertebrates during a transgenerational study, using brine shrimp Artemia as a model system. Sci Rep 6:21166 (2016). Read more (PubMed: 26876951) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer



1. Yes, it is possible for tissue or cell pellets to be frozen before extracting histone proteins.

2. The minimum amount of histone protein required as input for these assays are 50 ng (both for the colorimetric and fluorometric kits). In principle, 50 ng of histone proteins could be obtained from as little as 100,000 cells so pooling of blood may not be necessary. However we recommend using 1 million cells if possible in order to obtain a suitable amount of histone proteins for the assay.

3. We recommend using any standard protein quantification assay such as a Bradford assay for histone quantification.

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