Histone H4 Total Quantification Kit (Colorimetric) (ab156909)

Overview

  • Product name
    Histone H4 Total Quantification Kit (Colorimetric)
    See all Histone H4 kits
  • Sample type
    Cell culture extracts, Tissue Extracts, Nuclear Extracts
  • Assay type
    Quantitative
  • Sensitivity
    >= 0.5 ng/well
  • Range
    50 ng - 1000 ng
  • Assay time
    3h 30m
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Product overview

    Abcam's Histone H4 Total Quantification Kit (Colorimetric) is a complete set of essential components which enables an experimenter to colorimetrically quantify levels of histone H4 proteins independent of their modified states. It can also be used for normalizing the modified histone H4 content of samples when run in parallel with Abcam's histone modification quantification kit series.

    The kit is suitable for specifically measuring total histone H4 using human, mouse, and rat samples, including fresh and frozen tissues and cultured adherent and suspension cells.

Properties

  • Storage instructions
    Please refer to protocols.
  • Components 48 tests 96 tests
    1000X Capture Antibody 1 x 5µl 1 x 10µl
    10X Wash Buffer 1 x 14ml 1 x 28ml
    2000X Signal Reporter 1 x 6µl 1 x 12µl
    8-Well Assay Strips (with Frame) 1 x 6 units 1 x 12 units
    Color Developer 1 x 5ml 1 x 10ml
    Enhancer Solution 1 x 6µl 1 x 12µl
    Histone Assay Buffer 1 x 4ml 1 x 8ml
    Standard Control, 100 µg/mL 1 x 10µl 1 x 20µl
    Stop Solution 1 x 5ml 1 x 10ml
  • Research areas
  • Relevance
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Cellular localization
    Nuclear
  • Alternative names
    • H4/m
    • H4FA
    • H4FM
    • H4M
    • HIST1H4A
    see all
  • Database links

Images

  • Total H4 per mg of extracted histones from HeLa control cells (C) or cells treated with 10 micromolar sodium butyrate (But) (extracted using ab113476; 50-500 ng per well) (duplicates +/- SD).

  • Total H4 per mg of extracted histones from murine tissue preparations (extracted using ab113476; 50-500 ng per well) (duplicates +/- SD).

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Histone nuclear extracts were prepared from MDA-231 cells using the Histone Extraction Kit (ab113476) and the ODs generated from Histone H4 are measured.

  • Figure 1. Illustrated standard curve generated with H4 standard.

Protocols

References

ab156909 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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