Overview

  • Product name

    Anti-Histone H4 (tri methyl K20) antibody
    See all Histone H4 primary antibodies
  • Description

    Rabbit polyclonal to Histone H4 (tri methyl K20)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Dot blot, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Xenopus laevis
  • Immunogen

    Synthetic peptide within Human Histone H4 (N terminal) (tri methyl K20). The exact sequence is proprietary. Conjugated to a carrier protein.
    Database link: P62805

  • Positive control

    • WB: HeLa whole cell and nuclear lysates. IHC-P: Mouse duodenum, liver and muscle tissues; Rat fore brain tissue. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 11 kDa.
IHC-P 1/100 - 1/1000.
Dot blot 1/5000.
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-Histone H4 (tri methyl K20) antibody (ab227884) at 1/2000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 11 kDa



    15 % SDS-PAGE gel.

  • Paraffin-embedded mouse duodenum tissue stained for Histone H4 (tri methyl K20) using ab227884 at 1/500 dilution in immunohistochemical analysis.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H4 (tri methyl K20) (green) using ab227884 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Red: alpha Tubulin stained with an alpha Tubulin antibody at 1/500 dilution..
    Blue: Hoechst 33342 staining.

  • Paraffin-embedded mouse liver tissue stained for Histone H4 (tri methyl K20) using ab227884 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse muscle tissue stained for Histone H4 (tri methyl K20) using ab227884 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat fore brain tissue stained for Histone H4 (tri methyl K20) using ab227884 at 1/500 dilution in immunohistochemical analysis.

  • Dot Blot analysis of anti-Histone H4 (tri methyl Lys20) antibody with peptide samples.

    Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with ab227884 at 1/5000 dilution.

    A: Peptide samples of H4K20 un
    B: Peptide samples of H4K20me1
    C: Peptide samples of H4K20me2
    D: Peptide samples of H4K20me3

References

ab227884 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227884.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up