Key features and details
- Assay type: Enzyme activity
- Platform: Microplate reader
- Assay time: 3 hr
- Sample type: Cell Lysate, Tissue Extracts
Product nameHistone Demethylase (H3K4) Activity Quantification Assay Kit
Sample typeTissue Extracts, Cell Lysate
Assay typeEnzyme activity
Assay time3h 00m
Species reactivityReacts with: Mouse, Human, Plants
Predicted to work with: Mammals, Other species
Histone Demethylase (H3K4) Activity Quantification Assay Kit (ab113455) allows the user to measure activity/inhibition of H3K4 specific histone demethylases using cell/ tissue extracts. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase H3K4 specific activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.
Methylation of histone H3 at lysine 4 (H3K4) is thought to be a global epigenetic mark in euchromatin and a mediator of activated transcription. H3K4 demethylation is regulated by KDM1/LSD1, which can remove di- and mono-methylation from H3K4, and the JARID proteins such as RBP2 which catalyzes the removal of methylation through a hydroxylation reaction.
Storage instructionsPlease refer to protocols.
Components 48 tests 96 tests 10X Wash Buffer 1 x 15ml 1 x 30ml 8-Well Assay Strips (with Frame) 6 units 12 units Capture Antibody, 1000 µg/mL 1 x 5µl 1 x 10µl Detection Antibody, 200 µg/mL 1 x 8µl 1 x 16µl Fluoro Developer 1 x 12µl 1 x 24µl Fluoro Dilution 1 x 4ml 1 x 8ml Fluoro Enhancer 1 x 12µl 1 x 24µl HDM Assay Buffer 1 x 1.5ml 1 x 3ml HDM Standard, 20 µg/mL 1 x 25µl 1 x 50µl HDM Substrate 1 x 50µl 1 x 100µl
Nuclear extracts were prepared from Hela cells using the ab113475 and H3K4 specific histone demethyase activity was measured.
ab113455 has not yet been referenced specifically in any publications.