Overview

  • Product name

    HIV-1 Protease Activity Assay Kit (Fluorometric)
    See all HIV1 Protease kits
  • Detection method

    Fluorescent
  • Sample type

    Purified protein, Tissue Lysate
  • Assay type

    Quantitative
  • Product overview

    HIV-1 Protease Activity Assay Kit (Fluorometric) (ab211105) provides a convenient and simple method for detecting the activity of recombinant and/or purified HIV-1 protease. The assay kit is based on the ability of active HIV-1 protease to cleave a synthetic peptide substrate to release the free fluorophore which can be easily quantified at Ex/Em = 330/450 nm using a fluorescence microplate reader.


     

  • Notes

    Human Immunodeficiency Virus (HIV) is the cause of the Acquired Immunodeficiency Syndrome (AIDS). HIV-1 protease is a retroviral aspartyl protease (retropepsin) that is essential for the life-cycle of the virus as it cleaves newly synthesized polyproteins at the appropriate places to create the mature protein components of an infectious HIV virion. Without effective HIV1 protease, HIV virions remain non-infectious.

  • Platform

    Microplate reader

Properties

Images

  • Typical Fl. standard calibration curve.

  • Kinetic progress curves observed for increasing amounts of HIV-1 Protease (positive control, included in the kit) through time.

  • Measurement of HIV-1 Protease activity using increasing HIV-1 Protease positive control included in the kit, calculated from data from kinetic curves.

  • Measurement of HIV-1 Protease activity using HIV-1 Protease included in kit as positive control and activity of HIV-1 Protease in presence of Pepstatin A (protease inhibitor).

Protocols

References

ab211105 has not yet been referenced specifically in any publications.

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