Overview

  • Product name

    HIV-1 Protease Inhibitor Assay Kit (Fluorometric)
    See all HIV1 Protease kits
  • Detection method

    Fluorescent
  • Sample type

    Inhibitor compounds
  • Assay type

    Semi-quantitative
  • Product overview

    HIV-1 Protease Inhibitor Screening Kit (Fluorometric) (ab211106) provides a rapid, simple test suitable for high-throughput screening of HIV-1 Protease inhibitors. The assay is based on the ability of active HIV-1 protease to cleave a synthetic peptide substrate to release a free fluorophore, which can be easily quantified using a fluorescence microplate reader. In the presence of a HIV-1 Protease inhibitor, the cleavage of the substrate is reduced/abolished, resulting in decrease or total loss of the fluorescence. 


    This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of HIV-1 Protease.

  • Notes

    Human Immunodeficiency Virus (HIV) is the cause of the Acquired Immunodeficiency Syndrome (AIDS). HIV-1 protease is a retroviral aspartyl protease (retropepsin) that is essential for the life-cycle of the virus as it cleaves newly synthesized polyproteins at the appropriate places to create the mature protein components of an infectious HIV virion. Without effective HIV-1 protease, HIV virions remain non-infectious.

  • Platform

    Microplate reader

Properties

Images

  • Typical inhibition curve of HIV-1 Protease activity by the protease inhibitor Pepstatin A (IC50 = 1.6 µM) Assay was performed following the kit protocol.

Protocols

References

ab211106 has not yet been referenced specifically in any publications.

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