Anti-HIV protease antibody (ab211627)
Key features and details
- Rabbit polyclonal to HIV protease
- Suitable for: ELISA, WB, IP, Inhibition Assay
- Reacts with: Human immunodeficiency virus
- Isotype: IgG
Overview
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Product name
Anti-HIV protease antibody
See all HIV protease primary antibodies -
Description
Rabbit polyclonal to HIV protease -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WB, IP, Inhibition Assaymore details -
Species reactivity
Reacts with: Human immunodeficiency virus -
Immunogen
Recombinant full length protein corresponding to HIV protease. Full-size functional recombinant HIV1 protease expressed and purified from E. coli.
Database link: P03367 -
Positive control
- Purified HIV1 Protease; HIV1 (LAI strain) infected MT4 cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.09% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab211627 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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|
WB |
1/2000.
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|
IP |
1/200.
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|
Inhibition Assay |
Use at an assay dependent concentration.
Inhibotion of HIV1 protease activity |
Notes |
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ELISA
Use at an assay dependent concentration. |
WB
1/2000. |
IP
1/200. |
Inhibition Assay
Use at an assay dependent concentration. Inhibotion of HIV1 protease activity |
Target
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Relevance
The HIV1 core consists of a viral genome housed within a conical viral capsid that is generated during virion maturation. Human immunodeficiency virus type 1 (HIV1) matures after the viral protease processes the Gag and Pol polyproteins at 10 substrate locations. The protease of HIV1 is an aspartic protease and is functional only as a dimer; dimerization results in the formation of a binding cleft in which each of the two catalytic aspartic acids in which each monomer contributes each of the 2 catalytic aspartic acids. Because the protease is active only as a dimer, two of the GagPol precursors must themselves dimerize during virus assembly so that their protease domains can dimerize, become active, and process the precursors. Both the order and kinetics of cleavage as well as the extent of precursor processing appear to be critical steps in the generation of fully infectious, appropriately assembled viral particles. Inhibition of HIV-1 protease represents an important avenue for antiviral therapy. Currently available combination chemotherapy with reverse transcriptase inhibitors (RTIs) and protease inhibitors (PIs) for human immunodeficiency virus type 1 (HIV1) infection and AIDS have been shown to suppress the replication of HIV1 and extend the life expectancy of HIV1 infected individuals. -
Database links
- GenBank: AF459156 Human immunodeficiency virus
- SwissProt: P03366 Human immunodeficiency virus
- SwissProt: P03369 Human immunodeficiency virus
- SwissProt: Q9YQ30 Human immunodeficiency virus
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Alternative names
- HIV-1 protease antibody
- Human immunodeficiency virus protease antibody
- PR antibody
- Retropepsin antibody
Images
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab211627 has been referenced in 1 publication.
- Imamichi T et al. Natural Occurring Polymorphisms in HIV-1 Integrase and RNase H Regulate Viral Release and Autoprocessing. J Virol 95:e0132321 (2021). PubMed: 34523971