Anti-HIV1 gp120 antibody (ab106578)

Reviews (1)Q&A (6)
Western blot - Anti-HIV1 gp120 antibody (ab106578)
  • Western blot - Anti-HIV1 gp120 antibody (ab106578)

Key features and details

  • Rabbit polyclonal to HIV1 gp120
  • Suitable for: WB
  • Isotype: IgG

Overview

  • Product name

    Anti-HIV1 gp120 antibody
    See all HIV1 gp120 primary antibodies

  • Description

    Rabbit polyclonal to HIV1 gp120

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Other species

  • Immunogen

    16 amino acid peptide representing the major HIV1 consensus sequence near the N terminal region of V1/V2 loop (GenBank accession no. AAT67551).

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab106578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 120 kDa.

Target

  • Relevance

    HIV1 is equipped with the envelope gp160 glycoprotein for interaction with Langerhans cells (LCs) and dendritic cells (DCs), the members of the innate immune system, which confront the virus at the portal of virus entry in the human body. These cells are equipped with receptors by which they bind and endocytose the virus. The gp120 glycoprotein is used for binding to CD4 receptor and CCR5 co-receptor of T helper 2 (Th2) cells, and is able to induce FcepsilonRI(+) hematopoietic cells to produce IL4, which inactivates the host adaptive immune response.

  • Cellular localization

    Envelope surface glycoprotein.

  • Alternative names

    • Glycoprotein 120 antibody
    • gp120 antibody
    • SU antibody
    • Surface protein antibody

Images

  • Western blot - Anti-HIV1 gp120 antibody (ab106578)
    Western blot - Anti-HIV1 gp120 antibody (ab106578)
    Western Blot of HIV1 gp120 (5ng) labeling HIV1 gp120 with Anti-HIV1 gp120 antibody (ab106578) at 1µg/ml.
  • Western blot - Anti-HIV1 gp120 antibody (ab106578)
    Western blot - Anti-HIV1 gp120 antibody (ab106578)
    Lane 1 : Anti-HIV1 gp120 antibody (ab106578) at 1 µg/ml
    Lane 2 : Anti-HIV1 gp120 antibody (ab106578) at 2 µg/ml

    All lanes : gp120 protein

    Lysates/proteins at 1 µg per lane.

    Predicted band size: 120 kDa

References (0)

Publishing research using ab106578? Please let us know so that we can cite the reference in this datasheet.

ab106578 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-7 of 7 Abreviews or Q&A

ELISA abreview for Anti-HIV1 gp120 antibody

 Good
Abreviews
abreview image
Application
ELISA
Sample
Rabbit Recombinant protein (gp120)
Specification
gp120
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Type
Direct
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Kami Krista

Verified customer

Submitted Sep 30 2016

Question

Thank you for answer and help. I will try again with more normal brain samples and with reduction of protein. I loaded 20-30 ug of brain lysate and 1.5 ul from ur recombinant protein as positive control. I will repeat again and let you know maybe we can get one more gp120 gt ab in exchange of rabbit one or I will look for any other product that we may need from Abcam. I will keep you updated. The strong band that we got with GP120 looks like specific but the problem is it appear in normal brain too.

Thanks a lot,

Read More
Answer

Thank you very much for your reply.

Please keep me updated about the results after making those changes. I'll be happy to send another GP120 antibody or any other antibody that you might need.

I look forward to hearing from you. If there is anything else we can do for you, please let me know and I'll be happy to help.

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Question


I have tested the protein that you sent me and I attached the results.
Both abs that I have from Abcam binds the recombinant protein GP120 ( as two bands) but it looks like for the brain lysates rb GP120 is non specific ( got same pattern small intesity bands in cell lysates).
Gt GP120 it recognizes a band around 60-70 kDa ( that I thought is unglycosilated GP120 )but the problem is is binds also in the normal brain lysates ( any guess or suggestions?)
I attached the figures and if I would have a scientific input on this I will really appreciated. Regarding the antibodies, could we try more if you have any samples available?
I have use as negative control SKN Sh cell line and normal brain lysate. All dilutions were 1:1000.
Thank you,

Read More
Answer

Thank you very much for your email and for keeping me updated about the results with the recombinant protein.

I am sorry to see that the results are still not as expected. From your images, it seems that the bands in both of the brain lysates are non-specific, since the same bands are appearing in infected and non-infected samples. The banding pattern in the brain lysates is also different using ab21179 and ab106578, which also tells me this is non-specific binding. Has the GP120 titer been measured in the infected brain sample? My only explanation is that the level of the antigen may be below the detection limit of the antibody, but I'm not sure that is the case.

The GP120 protein ab69717 might have some degradation products or impurities at lower molecular weights as well, which appear as the bands around 40 and 55 kDa. How much of this protein was loaded per lane? We suggest 0.1-1 ug/lane, and loading less protein should decrease the visibility of these bands.

We don't have any samples of our antibodies, but I'd be happy to send you a replacement antibody if it will be helpful for you. Which antibody would you like to try?

I look forward to hearing from you. If there is anything else that we can do for you, please let me know and I'll be happy to help.

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Question

Just as a reminder could you please check is this GO120 recombinant protein is good for WB and how much I should load to get the signal?

Thank you.

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Answer

Thank you very much for your replies yesterday.

I'm sending a free of charge vial of ab69717 on the order *** which should arrive tomorrow. This protein has been tested in Western blot using 0.1-1 ug per lane. Please keep me updated about the results with the antibodies in WB against this protein.

I look forward to hearing from you. If there is anything else that we can do for you, please let me know and I'll be happy to help.

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Question

Thank you for your answer. As we talked a few days ago about GP120 ab, I may need to run some GP120 recombinant protein as control for my WB.

I just figures out that I have a very little amount in the lab. I was wondering if you could help me with some that I can use it to validate the GP120 ab.

Thank you so much,

Read More
Answer

Thank you very much for your reply.

I can send a vial of one of our GP120 proteins to use as a positive control. Would ab69717 work for you?

https://www.abcam.com/HIV1-gp120-protein-ab69717.html

Please confirm this and I will send a vial to you promptly. If there is anything else that we can do for you, please don't hesitate to ask.

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Question

75-80 kDa band with ab21179 and non-specific bands with ab106578 using infected patient brain lysates.

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Answer

Thank you very much for your call today and for letting us know about the trouble with these antibodies.

As we discussed, please keep me updated about the results after trying the antibodies with the recombinant protein. We do fully guarantee these antibodies to work, and I'll be happy to send replacements or issue credits or refunds if you prefer.

I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

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Question

Can you tell me the exact immunogen sequence of this product, HIV1 gp120 antibody (4641) please? If the information is proprietary, then could you please inform if the product can detect HIV-1 gp120 in JRFL lysates, and NL4-3 lysates? Ideally, by knowing the immunogen sequence, this can be confirmed.

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Answer

Thank you for your enquiry. Unfortunately, the immunogen information for this product is proprietary and cannot be released. However, an alignment with accession numbers Q75755 (JRFL) and D1G2M6 (NL4-3) was performed but there was very low homology with 18% and 6%, respectively.

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