Anti-HIV1 gp120 antibody (ab106578)

Abreviews (1)Q&A (6)

Overview

  • Product name
    Anti-HIV1 gp120 antibody
    See all HIV1 gp120 primary antibodies
  • Description
    Rabbit polyclonal to HIV1 gp120
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Other species
  • Immunogen

    16 amino acid peptide representing the major HIV1 consensus sequence near the N terminal region of V1/V2 loop (GenBank accession no. AAT67551).

Properties

Applications

Our Abpromise guarantee covers the use of ab106578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 120 kDa.

Target

  • Relevance
    HIV1 is equipped with the envelope gp160 glycoprotein for interaction with Langerhans cells (LCs) and dendritic cells (DCs), the members of the innate immune system, which confront the virus at the portal of virus entry in the human body. These cells are equipped with receptors by which they bind and endocytose the virus. The gp120 glycoprotein is used for binding to CD4 receptor and CCR5 co-receptor of T helper 2 (Th2) cells, and is able to induce FcepsilonRI(+) hematopoietic cells to produce IL4, which inactivates the host adaptive immune response.
  • Cellular localization
    Envelope surface glycoprotein.
  • Alternative names
    • Glycoprotein 120 antibody
    • gp120 antibody
    • SU antibody
    • Surface protein antibody

Images

  • Western blot - Anti-HIV1 gp120 antibody (ab106578)
    Western blot - Anti-HIV1 gp120 antibody (ab106578)
    Western Blot of HIV1 gp120 (5ng) labeling HIV1 gp120 with Anti-HIV1 gp120 antibody (ab106578) at 1µg/ml.
  • Western blot - Anti-HIV1 gp120 antibody (ab106578)
    Western blot - Anti-HIV1 gp120 antibody (ab106578)
    Lane 1 : Anti-HIV1 gp120 antibody (ab106578) at 1 µg/ml
    Lane 2 : Anti-HIV1 gp120 antibody (ab106578) at 2 µg/ml

    All lanes : gp120 protein

    Lysates/proteins at 1 µg per lane.

    Predicted band size: 120 kDa

References

ab106578 has not yet been referenced specifically in any publications.

Publishing research using ab106578? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

7 reviews or Q&A

10
Votes: Highest First

ELISA abreview for Anti-HIV1 gp120 antibody

 Good
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
ELISA
Sample
Rabbit Recombinant protein (gp120)
Specification
gp120
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Type
Direct
Read More
abreview image
Username

Kami Krista

Verified customer

Submitted Sep 30 2016

Thank you for answer and help. I will try again with more normal brain samples and with reduction of protein. I loaded 20-30 ug of brain lysate and 1.5 ul from ur recombinant protein as positive control. I will repeat again and let you know maybe we ca...

Read More

Thank you very much for your reply.

Please keep me updated about the results after making those changes. I'll be happy to send another GP120 antibody or any other antibody that you might need.

I look forward to hearing from you. If ...

Read More


I have tested the protein that you sent me and I attached the results.
Both abs that I have from Abcam binds the recombinant protein GP120 ( as two bands) but it looks like for the brain lysates rb GP120 is non specific ( got same pattern sma...

Read More

Thank you very much for your email and for keeping me updated about the results with the recombinant protein.

I am sorry to see that the results are still not as expected. From your images, it seems that the bands in both of the brain lysates...

Read More

Just as a reminder could you please check is this GO120 recombinant protein is good for WB and how much I should load to get the signal?

Thank you.

Thank you very much for your replies yesterday.

I'm sending a free of charge vial of ab69717 on the order *** which should arrive tomorrow. This protein has been tested in Western blot using 0.1-1 ug per lane. Please keep me updated about the...

Read More

Thank you for your answer. As we talked a few days ago about GP120 ab, I may need to run some GP120 recombinant protein as control for my WB.

I just figures out that I have a very little amount in the lab. I was wondering if you could help m...

Read More

Thank you very much for your reply.

I can send a vial of one of our GP120 proteins to use as a positive control. Would ab69717 work for you?

https://www.abcam.com/HIV1-gp120-protein-ab69717.html

Please confirm this and I w...

Read More

75-80 kDa band with ab21179 and non-specific bands with ab106578 using infected patient brain lysates.

Thank you very much for your call today and for letting us know about the trouble with these antibodies.

As we discussed, please keep me updated about the results after trying the antibodies with the recombinant protein. We do fully guarantee...

Read More

Can you tell me the exact immunogen sequence of this product, HIV1 gp120 antibody (4641) please? If the information is proprietary, then could you please inform if the product can detect HIV-1 gp120 in JRFL lysates, and NL4-3 lysates? Ideally, by k...

Read More

Thank you for your enquiry. Unfortunately, the immunogen information for this product is proprietary and cannot be released. However, an alignment with accession numbers Q75755 (JRFL) and D1G2M6 (NL4-3) was performed but there was very low homology...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up