Overview

  • Product name
  • Description
    Goat polyclonal to HIV1 gp120
  • Host species
    Goat
  • Tested applications
    Suitable for: Conjugation, ELISA, WBmore details
  • Immunogen

    Purified native gp120 from strain IIIB.

Properties

Applications

Our Abpromise guarantee covers the use of ab21179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Conjugation Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
WB 1/1000. Predicted molecular weight: 120 kDa. Dilution optimised using Chromogenic detection

Target

  • Relevance
    HIV1 is equipped with the envelope gp160 glycoprotein for interaction with Langerhans cells (LCs) and dendritic cells (DCs), the members of the innate immune system, which confront the virus at the portal of virus entry in the human body. These cells are equipped with receptors by which they bind and endocytose the virus. The gp120 glycoprotein is used for binding to CD4 receptor and CCR5 co-receptor of T helper 2 (Th2) cells, and is able to induce FcepsilonRI(+) hematopoietic cells to produce IL4, which inactivates the host adaptive immune response.
  • Cellular localization
    Envelope surface glycoprotein.
  • Alternative names
    • Glycoprotein 120 antibody
    • gp120 antibody
    • SU antibody
    • Surface protein antibody

References

This product has been referenced in:
  • Domljanovic I  et al. Complexes of DNA with fluorescent dyes are effective reagents for detection of autoimmune antibodies. Sci Rep 7:1925 (2017). Read more (PubMed: 28507286) »
  • Safavieh M  et al. Paper microchip with a graphene-modified silver nano-composite electrode for electrical sensing of microbial pathogens. Nanoscale 9:1852-1861 (2017). Read more (PubMed: 27845796) »
See all 17 Publications for this product

Customer reviews and Q&As

1-10 of 13 Abreviews or Q&A

Answer

Laboratory has finally confirmed that these products cannot be supplied with Texas Red conjugation so I am sorry we are unable to honour your request this time.

We however have conjugation kits available, e.g.

https://www.abcam.com/EasyLink-Texas-Red-Conjugation-Kit-3-x-10µg-TR-ab102925.html

https://www.abcam.com/EasyLink-Texas-Red-Conjugation-Kit-3-x-100µg-TR-ab102924.html

https://www.abcam.com/EasyLink-Texas-Red-Conjugation-Kit-1-x-1mg-TR-ab102923.html

The other labels can found at the following link;

https://www.abcam.com/index.html?pageconfig=resource&rid=13148

Please read the FAQs as well before selecting the suitable primary antibody for conjugation.

https://www.abcam.com/index.html?pageconfig=resource&rid=13156

Read More

Answer

Thank you for contacting us. Theoretically your outline makes sense. However, we have not tested the products this way and cannot guarantee that the binding sites of the antibodies will not interfere with each other. I hope this is helpful. Please le me know if I can be of further assistance.

Read More

Answer

Thank you very much for your reply.

Please keep me updated about the results after making those changes. I'll be happy to send another GP120 antibody or any other antibody that you might need.

I look forward to hearing from you. If there is anything else we can do for you, please let me know and I'll be happy to help.

Read More

Answer

Thank you very much for your email and for keeping me updated about the results with the recombinant protein.

I am sorry to see that the results are still not as expected. From your images, it seems that the bands in both of the brain lysates are non-specific, since the same bands are appearing in infected and non-infected samples. The banding pattern in the brain lysates is also different using ab21179 and ab106578, which also tells me this is non-specific binding. Has the GP120 titer been measured in the infected brain sample? My only explanation is that the level of the antigen may be below the detection limit of the antibody, but I'm not sure that is the case.

The GP120 protein ab69717 might have some degradation products or impurities at lower molecular weights as well, which appear as the bands around 40 and 55 kDa. How much of this protein was loaded per lane? We suggest 0.1-1 ug/lane, and loading less protein should decrease the visibility of these bands.

We don't have any samples of our antibodies, but I'd be happy to send you a replacement antibody if it will be helpful for you. Which antibody would you like to try?

I look forward to hearing from you. If there is anything else that we can do for you, please let me know and I'll be happy to help.

Read More

Answer

Thank you very much for your replies yesterday.

I'm sending a free of charge vial of ab69717 on the order *** which should arrive tomorrow. This protein has been tested in Western blot using 0.1-1 ug per lane. Please keep me updated about the results with the antibodies in WB against this protein.

I look forward to hearing from you. If there is anything else that we can do for you, please let me know and I'll be happy to help.

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Answer

Thank you very much for your reply.

I can send a vial of one of our GP120 proteins to use as a positive control. Would ab69717 work for you?

https://www.abcam.com/HIV1-gp120-protein-ab69717.html

Please confirm this and I will send a vial to you promptly. If there is anything else that we can do for you, please don't hesitate to ask.

Read More

Answer

Thank you very much for your call today and for letting us know about the trouble with these antibodies.

As we discussed, please keep me updated about the results after trying the antibodies with the recombinant protein. We do fully guarantee these antibodies to work, and I'll be happy to send replacements or issue credits or refunds if you prefer.

I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

Read More

Answer

Unfortunately, it looks like we do not have the unconjugated form of ab53837 Anti-HIV1 antibody (HRP) in our catalogue.
However, we have one antibody which may interest you:

https://www.abcam.com/index.html?datasheet=21179 (or use the following: https://www.abcam.com/index.html?datasheet=21179).

It's a goat antibody as well, and has a good customer review in WB as well as four WB references.




It's a goat antibody as well, and has a good customer review in WB as well as four WB refferences.

Read More

Answer

Thank you for your enquiry and your interest in our products. I have conducted a search for you on our website and found several antibodies against HIV gp120. Although, none of them have been specifically listed for use in affinity purification (AP); two of them (ab21179 and ab36921) are suitable for conjugation experiments. I would suggest taking a look at the on-line product datasheets to see which one suits your need. ab21179: https://www.abcam.com/HIV1-gp120-antibody-ab21179.html ab36921: https://www.abcam.com/HIV1-antibody-ab36921.html I hope this helps and if I can assist further, please do not hesitate to contact me.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (10 ul Supernatant from PBMC culture containing vir)
Loading amount
10 cells
Specification
10 ul Supernatant from PBMC culture containing vir
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Mr. Hoku West-Foyle

Verified customer

Submitted Nov 06 2009

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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