Anti-HIV1 gp120 antibody (ab21179)

Reviews (1)Q&A (12)References (17)

Overview

  • Product name
    Anti-HIV1 gp120 antibody
    See all HIV1 gp120 primary antibodies
  • Description
    Goat polyclonal to HIV1 gp120
  • Host species
    Goat
  • Tested applications
    Suitable for: Conjugation, ELISA, WBmore details
  • Immunogen

    Purified native gp120 from strain IIIB.

Properties

Applications

Our Abpromise guarantee covers the use of ab21179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Conjugation Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
WB 1/1000. Predicted molecular weight: 120 kDa. Dilution optimised using Chromogenic detection

Target

  • Relevance
    HIV1 is equipped with the envelope gp160 glycoprotein for interaction with Langerhans cells (LCs) and dendritic cells (DCs), the members of the innate immune system, which confront the virus at the portal of virus entry in the human body. These cells are equipped with receptors by which they bind and endocytose the virus. The gp120 glycoprotein is used for binding to CD4 receptor and CCR5 co-receptor of T helper 2 (Th2) cells, and is able to induce FcepsilonRI(+) hematopoietic cells to produce IL4, which inactivates the host adaptive immune response.
  • Cellular localization
    Envelope surface glycoprotein.
  • Alternative names
    • Glycoprotein 120 antibody
    • gp120 antibody
    • SU antibody
    • Surface protein antibody

References

This product has been referenced in:
  • Domljanovic I  et al. Complexes of DNA with fluorescent dyes are effective reagents for detection of autoimmune antibodies. Sci Rep 7:1925 (2017). Read more (PubMed: 28507286) »
  • Safavieh M  et al. Paper microchip with a graphene-modified silver nano-composite electrode for electrical sensing of microbial pathogens. Nanoscale 9:1852-1861 (2017). Read more (PubMed: 27845796) »
See all 17 Publications for this product

Publishing research using ab21179? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-10 of 13 Abreviews or Q&A

Question

I am looking for about 5-mg of label goat anti-HIV 1 gp120 antibodies with TEXAS RED or HIV-1 gp120 with texas red. Could you also please inform me of the other types of labels you would be able to put onto goat anti-HIV gp120, for example; Phycoerythrin or PECy 5.5.
Kind regards,

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Answer

Laboratory has finally confirmed that these products cannot be supplied with Texas Red conjugation so I am sorry we are unable to honour your request this time.

We however have conjugation kits available, e.g.

https://www.abcam.com/EasyLink-Texas-Red-Conjugation-Kit-3-x-10µg-TR-ab102925.html

https://www.abcam.com/EasyLink-Texas-Red-Conjugation-Kit-3-x-100µg-TR-ab102924.html

https://www.abcam.com/EasyLink-Texas-Red-Conjugation-Kit-1-x-1mg-TR-ab102923.html

The other labels can found at the following link;

https://www.abcam.com/index.html?pageconfig=resource&rid=13148

Please read the FAQs as well before selecting the suitable primary antibody for conjugation.

https://www.abcam.com/index.html?pageconfig=resource&rid=13156

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Question

Hello,

We would like to immobilize Anti-HIV1 gp120 onto a surface to show efficacy in capturing HIV protein.

Our current thought is to:
1. Immobilize Anti-HIV1 gp120 antibody (Product # ab21179)
2.Capture HIV1 gp120 + gp41 protein (Product #ab68127)
3. Use the secondary protein, Anti-HIV1 gp41 (Product # ab68622) conjugated to HRP to measure and quantify the amount of captured protein.

Can you confirm that the three products (ab21179, ab68127, and ab68622) will interact as predicted?

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Answer

Thank you for contacting us. Theoretically your outline makes sense. However, we have not tested the products this way and cannot guarantee that the binding sites of the antibodies will not interfere with each other. I hope this is helpful. Please le me know if I can be of further assistance.

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Question

Thank you for answer and help. I will try again with more normal brain samples and with reduction of protein. I loaded 20-30 ug of brain lysate and 1.5 ul from ur recombinant protein as positive control. I will repeat again and let you know maybe we can get one more gp120 gt ab in exchange of rabbit one or I will look for any other product that we may need from Abcam. I will keep you updated. The strong band that we got with GP120 looks like specific but the problem is it appear in normal brain too.

Thanks a lot,

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Answer

Thank you very much for your reply.

Please keep me updated about the results after making those changes. I'll be happy to send another GP120 antibody or any other antibody that you might need.

I look forward to hearing from you. If there is anything else we can do for you, please let me know and I'll be happy to help.

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Question


I have tested the protein that you sent me and I attached the results.
Both abs that I have from Abcam binds the recombinant protein GP120 ( as two bands) but it looks like for the brain lysates rb GP120 is non specific ( got same pattern small intesity bands in cell lysates).
Gt GP120 it recognizes a band around 60-70 kDa ( that I thought is unglycosilated GP120 )but the problem is is binds also in the normal brain lysates ( any guess or suggestions?)
I attached the figures and if I would have a scientific input on this I will really appreciated. Regarding the antibodies, could we try more if you have any samples available?
I have use as negative control SKN Sh cell line and normal brain lysate. All dilutions were 1:1000.
Thank you,

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Answer

Thank you very much for your email and for keeping me updated about the results with the recombinant protein.

I am sorry to see that the results are still not as expected. From your images, it seems that the bands in both of the brain lysates are non-specific, since the same bands are appearing in infected and non-infected samples. The banding pattern in the brain lysates is also different using ab21179 and ab106578, which also tells me this is non-specific binding. Has the GP120 titer been measured in the infected brain sample? My only explanation is that the level of the antigen may be below the detection limit of the antibody, but I'm not sure that is the case.

The GP120 protein ab69717 might have some degradation products or impurities at lower molecular weights as well, which appear as the bands around 40 and 55 kDa. How much of this protein was loaded per lane? We suggest 0.1-1 ug/lane, and loading less protein should decrease the visibility of these bands.

We don't have any samples of our antibodies, but I'd be happy to send you a replacement antibody if it will be helpful for you. Which antibody would you like to try?

I look forward to hearing from you. If there is anything else that we can do for you, please let me know and I'll be happy to help.

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Question

Just as a reminder could you please check is this GO120 recombinant protein is good for WB and how much I should load to get the signal?

Thank you.

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Answer

Thank you very much for your replies yesterday.

I'm sending a free of charge vial of ab69717 on the order *** which should arrive tomorrow. This protein has been tested in Western blot using 0.1-1 ug per lane. Please keep me updated about the results with the antibodies in WB against this protein.

I look forward to hearing from you. If there is anything else that we can do for you, please let me know and I'll be happy to help.

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Question

Thank you for your answer. As we talked a few days ago about GP120 ab, I may need to run some GP120 recombinant protein as control for my WB.

I just figures out that I have a very little amount in the lab. I was wondering if you could help me with some that I can use it to validate the GP120 ab.

Thank you so much,

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Answer

Thank you very much for your reply.

I can send a vial of one of our GP120 proteins to use as a positive control. Would ab69717 work for you?

https://www.abcam.com/HIV1-gp120-protein-ab69717.html

Please confirm this and I will send a vial to you promptly. If there is anything else that we can do for you, please don't hesitate to ask.

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Question

75-80 kDa band with ab21179 and non-specific bands with ab106578 using infected patient brain lysates.

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Answer

Thank you very much for your call today and for letting us know about the trouble with these antibodies.

As we discussed, please keep me updated about the results after trying the antibodies with the recombinant protein. We do fully guarantee these antibodies to work, and I'll be happy to send replacements or issue credits or refunds if you prefer.

I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

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Question

I have used Ab53837 successfully for Western blots and am interested in a non-conjugated version of this Ab. I see at least a couple goat polyclonal anti-gp120 antisera but I can't tell if there is a product that is identical to 53837 without the HRP. Can you help me with this please? Thanks in advance.

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Answer

Unfortunately, it looks like we do not have the unconjugated form of ab53837 Anti-HIV1 antibody (HRP) in our catalogue.
However, we have one antibody which may interest you:

https://www.abcam.com/index.html?datasheet=21179 (or use the following: https://www.abcam.com/index.html?datasheet=21179).

It's a goat antibody as well, and has a good customer review in WB as well as four WB references.




It's a goat antibody as well, and has a good customer review in WB as well as four WB refferences.

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Question

Dear Sir or Madame,   I am looking for a HIV gp120 specific antibody and came across your product list. I would like to use it in an affinity column (using Dynabeads, Invitrogen) to purify gp120 and was wondering if you can recommend a specific antibody of your range for this purpose.     Thanks a lot for your help.   Kind regards,

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Answer

Thank you for your enquiry and your interest in our products. I have conducted a search for you on our website and found several antibodies against HIV gp120. Although, none of them have been specifically listed for use in affinity purification (AP); two of them (ab21179 and ab36921) are suitable for conjugation experiments. I would suggest taking a look at the on-line product datasheets to see which one suits your need. ab21179: https://www.abcam.com/HIV1-gp120-antibody-ab21179.html ab36921: https://www.abcam.com/HIV1-antibody-ab36921.html I hope this helps and if I can assist further, please do not hesitate to contact me.

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Western blot abreview for Anti-HIV1 gp120 antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (10 ul Supernatant from PBMC culture containing vir)
Loading amount
10 cells
Specification
10 ul Supernatant from PBMC culture containing vir
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Mr. Hoku West-Foyle

Verified customer

Submitted Nov 06 2009

1-10 of 13 Abreviews or Q&A

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