Anti-HIV1 gp41 antibody [10E9] (ab9065)
Key features and details
- Mouse monoclonal [10E9] to HIV1 gp41
- Suitable for: WB, Radioimmunoprecipitation, ELISA, ICC/IF
- Isotype: IgG1
Overview
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Product name
Anti-HIV1 gp41 antibody [10E9]
See all HIV1 gp41 primary antibodies -
Description
Mouse monoclonal [10E9] to HIV1 gp41 -
Host species
Mouse -
Specificity
Reacts with Human Immunodeficiency Virus Type 1(HIV 1) gp41 envelope transmembrane protein. Also exhibits strong reactivity with HIV 1 env gene precursor gp160 using Western blotting. -
Tested applications
Suitable for: WB, Radioimmunoprecipitation, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Whole HIV viral lysate (B-3 strain). The epitope has not been mapped.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from serum-free culture supernatant. -
Clonality
Monoclonal -
Clone number
10E9 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | ||
Radioimmunoprecipitation | ||
ELISA | ||
ICC/IF |
Notes |
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ICC/IF: Use at a concentration of 1 - 10 mg/ml.
RipA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 10 µg/ml.
Antibody dilutions should be prepared using buffers containing suitable protein in order to stabilize antibody activity.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
gp41/120 is the major HIV protein associated with the HIV envelope. It functions as the viral antireceptor or attachment protein. gp41 (or TM) traverses the envelope, whereas gp120 is present on the outer surface and is noncovalently attached to gp41. The precursor of gp120/41 (gp160) is synthesized in the endoplasmic reticulum and is transported via the golgi body to the cell surface. Upon activation of the envelope glycoprotein (gp120/41) by cellular receptors, gp41 undergoes conformational changes that mediate fusion of the viral and cellular membranes. -
Alternative names
- Env antibody
- Glycoprotein 41 antibody
- gp41 antibody
see all
Protocols
Datasheets and documents
References (2)
ab9065 has been referenced in 2 publications.
- Lebohang Manoto S et al. Exploring optical spectroscopic techniques and nanomaterials for virus detection. Saudi J Biol Sci N/A:N/A (2020). PubMed: 32868971
- Papsidero LD et al. Monoclonal antibody identifies a highly conserved and immunodominant epitope of the human immunodeficiency virus transmembrane protein. Hybridoma 7:117-28 (1988). PubMed: 2453451