Anti-HIV1 gp41 antibody [10E9] (ab9065)

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Overview

  • Product name
    Anti-HIV1 gp41 antibody [10E9]
    See all HIV1 gp41 primary antibodies
  • Description
    Mouse monoclonal [10E9] to HIV1 gp41
  • Host species
    Mouse
  • Specificity
    Reacts with Human Immunodeficiency Virus Type 1(HIV 1) gp41 envelope transmembrane protein. Also exhibits strong reactivity with HIV 1 env gene precursor gp160 using Western blotting.
  • Tested applications
    Suitable for: WB, Radioimmunoprecipitation, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Other species
  • Immunogen

    Whole HIV viral lysate (B-3 strain). The epitope has not been mapped.

Properties

Applications

Our Abpromise guarantee covers the use of ab9065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Radioimmunoprecipitation
ELISA
ICC/IF
  • Application notes
    ELISA: Use at an assay dependent dilution.
    ICC/IF: Use at a concentration of 1 - 10 mg/ml.
    RipA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 10 µg/ml.

    Antibody dilutions should be prepared using buffers containing suitable protein in order to stabilize antibody activity.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Relevance
      gp41/120 is the major HIV protein associated with the HIV envelope. It functions as the viral antireceptor or attachment protein. gp41 (or TM) traverses the envelope, whereas gp120 is present on the outer surface and is noncovalently attached to gp41. The precursor of gp120/41 (gp160) is synthesized in the endoplasmic reticulum and is transported via the golgi body to the cell surface. Upon activation of the envelope glycoprotein (gp120/41) by cellular receptors, gp41 undergoes conformational changes that mediate fusion of the viral and cellular membranes.
    • Alternative names
      • Env antibody
      • Glycoprotein 41 antibody
      • gp41 antibody
      • Human Immunodeficiency Virus 1 antibody
      • TM antibody
      • Transmembrane protein antibody
      see all

    References

    This product has been referenced in:
    • Papsidero LD  et al. Monoclonal antibody identifies a highly conserved and immunodominant epitope of the human immunodeficiency virus transmembrane protein. Hybridoma 7:117-28 (1988). Read more (PubMed: 2453451) »
    See 1 Publication for this product

    Publishing research using ab9065? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Question

    ab9065 and ab18633 (gp41 antibodies) Will these products recognize HIV strain LAV in WB?

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    Answer

    Thank you for your patience in this matter. I have received replies from the originators of ab9065 and ab18633. In regards to ab9065, this antibody was produced to the HIV-1 B-3 strain and its' reactivity with the LAV strain has not been tested in WB. Regarding ab18633, this antibody was raised to the HIV IIIB strain and its' reactivity with the LAV strain has not been determined. Please do not hesitate to contact us again if you have further questions.

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    Question

    What fixatives have been used successfully to get gp41 immunofluorescent staining of infected HIV-infected cells?

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    Answer

    At this time we do not have specific information regarding what type of fixatives were used for immunofluorescence staining, but we assume HIV infected cells were paraformaldehyde fixed to slides. They were then developed or stained with ab9065. We are not sure if a counterstain was used or what conjugate was used.

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    Question

    Hi, I wanted to know which epitope within gp41 does the antibody recognize? We have a fusion protein and only a certain region of the gp41 is present in this protein. I want to find out if i can use this antibody for detecting our fusion protein in immunofluorescence assay. Thanks, Yours truly, Himangi Jayakar

    Read More
    Answer

    The immunogen used is a whole HIV viral lysate and the antibody was identified as gp41 by Western blotting. The epitope has not been mapped.

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    Question

    Does this ab work on paraffin-embedded tissue? Larisa Poluektova

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    Answer

    This antibody has not yet been tested for use with paraffin embedded tissue.

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