For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    hiv1-gp41-antibody-10e9-ab9065.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Microbiology Organism Virus RNA Virus ssRNA positive strand virus HIV
Share by email

Anti-HIV1 gp41 antibody [10E9] (ab9065)

  • Datasheet
Submit a review Q&A (4)References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Mouse monoclonal [10E9] to HIV1 gp41
  • Suitable for: WB, Radioimmunoprecipitation, ELISA, ICC/IF
  • Isotype: IgG1

You may also be interested in

Primary
Biotin Anti-HIV1 p24 antibody (ab20774)
Primary
Anti-HIV1 gp120 antibody (ab21179)
Protein
Recombinant HIV1 gp41 protein (ab49068)

View more associated products

Overview

  • Product name

    Anti-HIV1 gp41 antibody [10E9]
    See all HIV1 gp41 primary antibodies
  • Description

    Mouse monoclonal [10E9] to HIV1 gp41
  • Host species

    Mouse
  • Specificity

    Reacts with Human Immunodeficiency Virus Type 1(HIV 1) gp41 envelope transmembrane protein. Also exhibits strong reactivity with HIV 1 env gene precursor gp160 using Western blotting.
  • Tested applications

    Suitable for: WB, Radioimmunoprecipitation, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Whole HIV viral lysate (B-3 strain). The epitope has not been mapped.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from serum-free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    10E9
  • Isotype

    IgG1
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • HIV
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant HIV1 gp41 protein (ab49068)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Radioimmunoprecipitation
ELISA
ICC/IF
Notes
  • Application notes
    ELISA: Use at an assay dependent dilution.
    ICC/IF: Use at a concentration of 1 - 10 mg/ml.
    RipA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 10 µg/ml.

    Antibody dilutions should be prepared using buffers containing suitable protein in order to stabilize antibody activity.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      gp41/120 is the major HIV protein associated with the HIV envelope. It functions as the viral antireceptor or attachment protein. gp41 (or TM) traverses the envelope, whereas gp120 is present on the outer surface and is noncovalently attached to gp41. The precursor of gp120/41 (gp160) is synthesized in the endoplasmic reticulum and is transported via the golgi body to the cell surface. Upon activation of the envelope glycoprotein (gp120/41) by cellular receptors, gp41 undergoes conformational changes that mediate fusion of the viral and cellular membranes.
    • Alternative names

      • Env antibody
      • Glycoprotein 41 antibody
      • gp41 antibody
      • Human Immunodeficiency Virus 1 antibody
      • TM antibody
      • Transmembrane protein antibody
      see all

    Protocols

    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (2)

    Publishing research using ab9065? Please let us know so that we can cite the reference in this datasheet.

    ab9065 has been referenced in 2 publications.

    • Lebohang Manoto S  et al. Exploring optical spectroscopic techniques and nanomaterials for virus detection. Saudi J Biol Sci N/A:N/A (2020). PubMed: 32868971
    • Papsidero LD  et al. Monoclonal antibody identifies a highly conserved and immunodominant epitope of the human immunodeficiency virus transmembrane protein. Hybridoma 7:117-28 (1988). PubMed: 2453451

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Question

    ab9065 and ab18633 (gp41 antibodies) Will these products recognize HIV strain LAV in WB?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 02 2007

    Answer

    Thank you for your patience in this matter. I have received replies from the originators of ab9065 and ab18633. In regards to ab9065, this antibody was produced to the HIV-1 B-3 strain and its' reactivity with the LAV strain has not been tested in WB. Regarding ab18633, this antibody was raised to the HIV IIIB strain and its' reactivity with the LAV strain has not been determined. Please do not hesitate to contact us again if you have further questions.

    Read More

    Abcam Scientific Support

    Answered on Jan 03 2007

    Question

    What fixatives have been used successfully to get gp41 immunofluorescent staining of infected HIV-infected cells?

    Read More

    Abcam community

    Verified customer

    Asked on May 27 2004

    Answer

    At this time we do not have specific information regarding what type of fixatives were used for immunofluorescence staining, but we assume HIV infected cells were paraformaldehyde fixed to slides. They were then developed or stained with ab9065. We are not sure if a counterstain was used or what conjugate was used.

    Read More

    Abcam Scientific Support

    Answered on Jun 01 2004

    Question

    Hi, I wanted to know which epitope within gp41 does the antibody recognize? We have a fusion protein and only a certain region of the gp41 is present in this protein. I want to find out if i can use this antibody for detecting our fusion protein in immunofluorescence assay. Thanks, Yours truly, Himangi Jayakar

    Read More

    Abcam community

    Verified customer

    Asked on Apr 23 2002

    Answer

    The immunogen used is a whole HIV viral lysate and the antibody was identified as gp41 by Western blotting. The epitope has not been mapped.

    Read More

    Simon Renshaw

    Abcam Scientific Support

    Answered on Apr 26 2002

    Question

    Does this ab work on paraffin-embedded tissue? Larisa Poluektova

    Read More

    Abcam community

    Verified customer

    Asked on Feb 22 2002

    Answer

    This antibody has not yet been tested for use with paraffin embedded tissue.

    Read More

    Abcam Scientific Support

    Answered on Feb 25 2002

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.