Key features and details
- Mouse monoclonal [IN-2] to HIV1 integrase
- Suitable for: WB, ICC/IF, IP
- Isotype: IgG1
Product nameAnti-HIV1 integrase antibody [IN-2]
DescriptionMouse monoclonal [IN-2] to HIV1 integrase
Specificityab66645 is specific for bacterial HIV1 integrase (IN) protein.
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Other species
Bacterially expressed, hexahistidine amino-terminal tagged HIV1 integrase (IN) protein (clade B, HXB-3 isolate)
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab66645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 32 kDa.|
|ICC/IF||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
RelevanceIntegrase is an enzyme, a product of the HIV1 Gag/Pol gene; the other two enzymes being reverse transcriptase and protease. The human immunodeficiency virus (HIV-1) uses an enzyme, a so-called integrase, to carry out the integration of its viral DNA into the host chromosome thereby tricking the host cell machinery into making viral proteins. The HIV-1 integrase is a protein of 32 kDa and is composed of three domains. No cellular homologue of HIV integrase has been described, so potential inhibitors to this enzyme could be relatively nontoxic.
- HIV antibody
- Human Immunodeficiency virus 1 antibody
- IN antibody
- Integrase antibody
ab66645 staining HIV1 integrase in transfected 293T by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol/acetone (1:1), permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/1000 in PBS + 1% BSA) for 1 hour at 21°C. An Alexa Fluor® 555-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody (1/2000).
ab66645 has been referenced in 6 publications.
- Takahata T et al. Critical Contribution of Tyr15 in the HIV-1 Integrase (IN) in Facilitating IN Assembly and Nonenzymatic Function through the IN Precursor Form with Reverse Transcriptase. J Virol 91:N/A (2017). PubMed: 27795445
- Hoyte AC et al. Resistance to pyridine-based inhibitor KF116 reveals an unexpected role of integrase in HIV-1 Gag-Pol polyprotein proteolytic processing. J Biol Chem 292:19814-19825 (2017). PubMed: 28972144
- Saleh S et al. HIV integration and the establishment of latency in CCL19-treated resting CD4(+) T cells require activation of NF-?B. Retrovirology 13:49 (2016). IP ; Human . PubMed: 27459960
- Desimmie BA et al. HIV-1 IN/Pol recruits LEDGF/p75 into viral particles. Retrovirology 12:16 (2015). PubMed: 25809198
- Borrenberghs D et al. HIV virions as nanoscopic test tubes for probing oligomerization of the integrase enzyme. ACS Nano 8:3531-45 (2014). PubMed: 24654558
- Desimmie BA et al. LEDGINs inhibit late stage HIV-1 replication by modulating integrase multimerization in the virions. Retrovirology 10:57 (2013). WB . PubMed: 23721378