Anti-HIV1 Nef antibody [3D12] (ab42355)
- Datasheet
- References (14)
- Protocols
Overview
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Product name
Anti-HIV1 Nef antibody [3D12]
See all HIV1 Nef primary antibodies -
Description
Mouse monoclonal [3D12] to HIV1 Nef -
Host species
Mouse -
Specificity
This antibody reacts with the rNEF of HIV 1 subtype B and C, -
Tested applications
Suitable for: ICC/IF, ELISA, WBmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Recombinant Nef protein of HIV1 HXB2.
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Epitope
Mapped to amino acids 35-50 (RDLEKHGAITSSNTAA - HIV1 HXB2) -
General notes
Also known as clone 01-002.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified by affinty chromatography. -
Clonality
Monoclonal -
Clone number
3D12 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab42355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| ELISA | 1/5000. | |
| WB | 1/500. Predicted molecular weight: 24 kDa. |
Target
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Relevance
Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. -
Alternative names
- C terminal core protein antibody
- F protein antibody
- Nef antibody
see all
Images
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![Western blot - Anti-HIV1 Nef antibody [3D12] (ab42355)](https://www.abcam.com/ps/products/42/ab42355/Images/ab42355-172785-anti-hiv1-nef-antibody-3d12-western-blot.jpg)
Western blot - Anti-HIV1 Nef antibody [3D12] (ab42355)Lane 1 : Anti-HIV1 Nef antibody [3D12] (ab42355)
Lane 1 : Recombinant HIV1 Nef (B subtype)
Lane 2 : Protein size marker
Predicted band size: 24 kDa
References
This product has been referenced in:
- Kumar P et al. HIV-1 Nef physically associate with CAMKIId - ASK-1 complex to inhibit p38MAPK signalling and apoptosis in infected cells. Life Sci 224:263-273 (2019). Read more (PubMed: 30902545) »
- Kumari S et al. HIV-1 Nef-GCC185 interaction regulates assembly of cellular protein complexes at TGN targeting MHC-I downregulation. Life Sci N/A:N/A (2019). Read more (PubMed: 30953643) »
