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Cell Biology Apoptosis Receptors Death Receptors
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Anti-HIV1 Nef antibody [3D12] (ab42355)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (15)

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Western blot - Anti-HIV1 Nef antibody [3D12] (ab42355)

    Key features and details

    • Mouse monoclonal [3D12] to HIV1 Nef
    • Suitable for: ICC/IF, ELISA, WB
    • Isotype: IgG1

    You may also be interested in

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    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
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    Recombinant HIV1 p24 protein (ab43037)
    Protein
    Product image
    Recombinant HIV1 Nef protein (ab63996)

    View more associated products

    Overview

    • Product name

      Anti-HIV1 Nef antibody [3D12]
      See all HIV1 Nef primary antibodies
    • Description

      Mouse monoclonal [3D12] to HIV1 Nef
    • Host species

      Mouse
    • Specificity

      This antibody reacts with the rNEF of HIV 1 subtype B and C,
    • Tested applications

      Suitable for: ICC/IF, ELISA, WBmore details
    • Species reactivity

      Reacts with: Other species
    • Immunogen

      Recombinant Nef protein of HIV1 HXB2.

    • Epitope

      Mapped to amino acids 35-50 (RDLEKHGAITSSNTAA - HIV1 HXB2)
    • General notes

      Also known as clone 01-002.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.40
      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Purification notes

      Purified by affinty chromatography.
    • Clonality

      Monoclonal
    • Clone number

      3D12
    • Isotype

      IgG1
    • Research areas

      • Cell Biology
      • Apoptosis
      • Receptors
      • Death Receptors
      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • ssRNA positive strand virus
      • HIV
      • Immunology
      • Immune System Diseases
      • Antiviral Signaling
      • Cancer
      • Oncoproteins/suppressors
      • Viral proteins
      • Retrovirus
      • Immunology
      • Immune System Diseases
      • Antiviral Signaling
      • HIV

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant HIV1 Nef protein (ab63996)

    Applications

    Our Abpromise guarantee covers the use of ab42355 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF Use at an assay dependent concentration.
    ELISA 1/5000.
    WB 1/500. Predicted molecular weight: 24 kDa.

    Target

    • Relevance

      Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation.
    • Alternative names

      • C terminal core protein antibody
      • F protein antibody
      • Nef antibody
      • Negative factor antibody
      • p27 antibody
      see all

    Images

    • Western blot - Anti-HIV1 Nef antibody [3D12] (ab42355)
      Western blot - Anti-HIV1 Nef antibody [3D12] (ab42355)
      Lane 1 : Anti-HIV1 Nef antibody [3D12] (ab42355)


      Lane 1 : Recombinant HIV1 Nef (B subtype)
      Lane 2 : Protein size marker

      Predicted band size: 24 kDa

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (15)

    Publishing research using ab42355? Please let us know so that we can cite the reference in this datasheet.

    ab42355 has been referenced in 15 publications.

    • Kumar P  et al. HIV-1 Nef physically associate with CAMKIId - ASK-1 complex to inhibit p38MAPK signalling and apoptosis in infected cells. Life Sci 224:263-273 (2019). PubMed: 30902545
    • Kumari S  et al. HIV-1 Nef-GCC185 interaction regulates assembly of cellular protein complexes at TGN targeting MHC-I downregulation. Life Sci N/A:N/A (2019). PubMed: 30953643
    • Sithole N  et al. DDX17 Specifically, and Independently of DDX5, Controls Use of the HIV A4/5 Splice Acceptor Cluster and Is Essential for Efficient Replication of HIV. J Mol Biol 430:3111-3128 (2018). PubMed: 30131116
    • Zhou F  et al. HIV-1 Nef-induced lncRNA AK006025 regulates CXCL9/10/11 cluster gene expression in astrocytes through interaction with CBP/P300. J Neuroinflammation 15:303 (2018). PubMed: 30382871
    • Mbonye U  et al. Cyclin-dependent kinase 7 (CDK7)-mediated phosphorylation of the CDK9 activation loop promotes P-TEFb assembly with Tat and proviral HIV reactivation. J Biol Chem 293:10009-10025 (2018). PubMed: 29743242
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    I had a customer request a certificate of analysis for AB24586 and AB42355. For AB24586, he specifically requests the CoA for lot #GR69388-1. For the AB42355, he specifically requests the CoA for lot #GR745883-1.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 19 2012

    Answer

    Thank you for your enquiry.

    Please find the 2 requested CoC documents attached.

    I hope this is helpful. Please contact us again if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on Jun 19 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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