Anti-HIV1 Nef antibody [3D12] (ab42355)
![Western blot - Anti-HIV1 Nef antibody [3D12] (ab42355)](https://www.abcam.com/ps/products/42/ab42355/Images/ab42355-172785-anti-hiv1-nef-antibody-3d12-western-blot.jpg "Western blot - Anti-HIV1 Nef antibody [3D12] (ab42355)")
Key features and details
- Mouse monoclonal [3D12] to HIV1 Nef
- Suitable for: ICC/IF, ELISA, WB
- Isotype: IgG1
Overview
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Product name
Anti-HIV1 Nef antibody [3D12]
See all HIV1 Nef primary antibodies -
Description
Mouse monoclonal [3D12] to HIV1 Nef -
Host species
Mouse -
Specificity
This antibody reacts with the rNEF of HIV 1 subtype B and C, -
Tested applications
Suitable for: ICC/IF, ELISA, WBmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Recombinant Nef protein of HIV1 HXB2.
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Epitope
Mapped to amino acids 35-50 (RDLEKHGAITSSNTAA - HIV1 HXB2) -
General notes
Also known as clone 01-002.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified by affinty chromatography. -
Clonality
Monoclonal -
Clone number
3D12 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab42355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| ELISA |
1/5000.
|
|
| WB |
1/500. Predicted molecular weight: 24 kDa.
|
| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
ELISA
1/5000. |
|
WB
1/500. Predicted molecular weight: 24 kDa. |
Target
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Relevance
Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. -
Alternative names
- C terminal core protein antibody
- F protein antibody
- Nef antibody
see all
Images
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Western blot - Anti-HIV1 Nef antibody [3D12] (ab42355)Lane 1 : Anti-HIV1 Nef antibody [3D12] (ab42355)
Lane 1 : Recombinant HIV1 Nef (B subtype)
Lane 2 : Protein size marker
Predicted band size: 24 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab42355 has been referenced in 17 publications.
- Duggan MR et al. Neuromodulation of BAG co-chaperones by HIV-1 viral proteins and H2O2: implications for HIV-associated neurological disorders. Cell Death Discov 7:60 (2021). PubMed: 33771978
- Ciccosanti F et al. High Levels of TRIM5a Are Associated with Xenophagy in HIV-1-Infected Long-Term Nonprogressors. Cells 10:N/A (2021). PubMed: 34069225
- Kumar P et al. HIV-1 Nef physically associate with CAMKIId - ASK-1 complex to inhibit p38MAPK signalling and apoptosis in infected cells. Life Sci 224:263-273 (2019). PubMed: 30902545
- Kumari S et al. HIV-1 Nef-GCC185 interaction regulates assembly of cellular protein complexes at TGN targeting MHC-I downregulation. Life Sci N/A:N/A (2019). PubMed: 30953643
- Sithole N et al. DDX17 Specifically, and Independently of DDX5, Controls Use of the HIV A4/5 Splice Acceptor Cluster and Is Essential for Efficient Replication of HIV. J Mol Biol 430:3111-3128 (2018). PubMed: 30131116