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  1. Link

    hiv1-nef-antibody-3f2-ab25866.pdf

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Microbiology Organism Virus RNA Virus ssRNA positive strand virus HIV
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Anti-HIV1 Nef antibody [3F2] (ab25866)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (6)

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Western blot - Anti-HIV1 Nef antibody [3F2] (ab25866)

    Key features and details

    • Mouse monoclonal [3F2] to HIV1 Nef
    • Suitable for: ELISA, WB, Radioimmunoprecipitation
    • Isotype: IgG1

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Protein
    Product image
    Recombinant HIV1 Nef protein (ab63996)

    View more associated products

    Overview

    • Product name

      Anti-HIV1 Nef antibody [3F2]
      See all HIV1 Nef primary antibodies
    • Description

      Mouse monoclonal [3F2] to HIV1 Nef
    • Host species

      Mouse
    • Tested applications

      Suitable for: ELISA, WB, Radioimmunoprecipitationmore details
    • Species reactivity

      Reacts with: Other species
    • Immunogen

      Recombinant full length protein

    • Epitope

      Mapped to amino acids 31-35 (GAASR, HIV-1 HxB2).
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.40
      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Monoclonal
    • Clone number

      3F2
    • Isotype

      IgG1
    • Research areas

      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • ssRNA positive strand virus
      • HIV
      • Immunology
      • Immune System Diseases
      • Antiviral Signaling
      • HIV

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant HIV1 Nef protein (ab63996)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab25866 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA
    Use at an assay dependent dilution.
    WB (1)
    Use at an assay dependent dilution. Predicted molecular weight: 23 kDa.
    Radioimmunoprecipitation
    Use at an assay dependent dilution.
    Notes
    ELISA
    Use at an assay dependent dilution.
    WB
    Use at an assay dependent dilution. Predicted molecular weight: 23 kDa.
    Radioimmunoprecipitation
    Use at an assay dependent dilution.

    Target

    • Relevance

      Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation.
    • Alternative names

      • C terminal core protein antibody
      • F protein antibody
      • Nef antibody
      • Negative factor antibody
      • p27 antibody
      see all

    Images

    • Western blot - Anti-HIV1 Nef antibody [3F2] (ab25866)
      Western blot - Anti-HIV1 Nef antibody [3F2] (ab25866)
      Lane 1 : Anti-HIV1 Nef antibody [3F2] (ab25866)


      Lane 1 : Recombinant HIV1 Nef (B subtype)
      Lane 2 : Protein size marker

      Predicted band size: 23 kDa

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (6)

    Publishing research using ab25866? Please let us know so that we can cite the reference in this datasheet.

    ab25866 has been referenced in 6 publications.

    • Kjerrström A  et al. Interactions of single and combined human immunodeficiency virus type 1 (HIV-1) DNA vaccines. Virology 284:46-61 (2001). PubMed: 11352667
    • Collings A  et al. Humoral and cellular immune responses to HIV-1 nef in mice DNA-immunised with non-replicating or self-replicating expression vectors. Vaccine 18:460-7 (1999). PubMed: 10519935
    • Ranki A  et al. Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS 9:1001-8 (1995). PubMed: 8527071
    • Saito Y  et al. Overexpression of nef as a marker for restricted HIV-1 infection of astrocytes in postmortem pediatric central nervous tissues. Neurology 44:474-81 (1994). PubMed: 8145918
    • Ovod V  et al. Immunological variation and immunohistochemical localization of HIV-1 Nef demonstrated with monoclonal antibodies. AIDS 6:25-34 (1992). PubMed: 1371924
    • Zsolnai B  et al. Effects of beta 2-adrenergic stimulants on the progesterone and oestradiol-17 beta serum levels in pseudopregnant rats. Acta Physiol Hung 77:7-11 (1991). PubMed: 1683091

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-HIV1 Nef antibody [3F2]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293T cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% gel)
    Loading amount
    10 µg
    Specification
    HEK293T cells
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    DR. Jason Lee

    Verified customer

    Submitted Aug 15 2016

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