Anti-HIV1 Nef antibody [3F2] (ab25866)
- Datasheet
- References (6)
- Protocols
Overview
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Product name
Anti-HIV1 Nef antibody [3F2]
See all HIV1 Nef primary antibodies -
Description
Mouse monoclonal [3F2] to HIV1 Nef -
Host species
Mouse -
Tested applications
Suitable for: ELISA, WB, Radioimmunoprecipitationmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Recombinant full length protein
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Epitope
Mapped to amino acids 31-35 (GAASR, HIV-1 HxB2).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
3F2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab25866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent dilution. | |
| WB | Use at an assay dependent dilution. Predicted molecular weight: 23 kDa. | |
| Radioimmunoprecipitation | Use at an assay dependent dilution. |
Target
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Relevance
Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. -
Alternative names
- C terminal core protein antibody
- F protein antibody
- Nef antibody
see all
Images
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![Western blot - Anti-HIV1 Nef antibody [3F2] (ab25866)](https://www.abcam.com/ps/products/25/ab25866/Images/ab25866-172784-A2801100-Figure-1.jpg)
Western blot - Anti-HIV1 Nef antibody [3F2] (ab25866)Lane 1 : Anti-HIV1 Nef antibody [3F2] (ab25866)
Lane 1 : Recombinant HIV1 Nef (B subtype)
Lane 2 : Protein size marker
Predicted band size: 23 kDa
References
This product has been referenced in:
- Kjerrström A et al. Interactions of single and combined human immunodeficiency virus type 1 (HIV-1) DNA vaccines. Virology 284:46-61 (2001). Read more (PubMed: 11352667) »
- Collings A et al. Humoral and cellular immune responses to HIV-1 nef in mice DNA-immunised with non-replicating or self-replicating expression vectors. Vaccine 18:460-7 (1999). Read more (PubMed: 10519935) »
