Anti-HIV1 Nef antibody [3F2] (ab25866)
![Western blot - Anti-HIV1 Nef antibody [3F2] (ab25866)](https://www.abcam.com/ps/products/25/ab25866/Images/ab25866-172784-A2801100-Figure-1.jpg "Western blot - Anti-HIV1 Nef antibody [3F2] (ab25866)")
Key features and details
- Mouse monoclonal [3F2] to HIV1 Nef
- Suitable for: ELISA, WB, Radioimmunoprecipitation
- Isotype: IgG1
Overview
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Product name
Anti-HIV1 Nef antibody [3F2]
See all HIV1 Nef primary antibodies -
Description
Mouse monoclonal [3F2] to HIV1 Nef -
Host species
Mouse -
Tested applications
Suitable for: ELISA, WB, Radioimmunoprecipitationmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Recombinant full length protein
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Epitope
Mapped to amino acids 31-35 (GAASR, HIV-1 HxB2). -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
3F2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent dilution.
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| WB | (1) |
Use at an assay dependent dilution. Predicted molecular weight: 23 kDa.
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| Radioimmunoprecipitation |
Use at an assay dependent dilution.
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| Notes |
|---|
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ELISA
Use at an assay dependent dilution. |
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WB
Use at an assay dependent dilution. Predicted molecular weight: 23 kDa. |
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Radioimmunoprecipitation
Use at an assay dependent dilution. |
Target
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Relevance
Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. -
Alternative names
- C terminal core protein antibody
- F protein antibody
- Nef antibody
see all
Images
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Western blot - Anti-HIV1 Nef antibody [3F2] (ab25866)Lane 1 : Anti-HIV1 Nef antibody [3F2] (ab25866)
Lane 1 : Recombinant HIV1 Nef (B subtype)
Lane 2 : Protein size marker
Predicted band size: 23 kDa
Protocols
References (6)
ab25866 has been referenced in 6 publications.
- Kjerrström A et al. Interactions of single and combined human immunodeficiency virus type 1 (HIV-1) DNA vaccines. Virology 284:46-61 (2001). PubMed: 11352667
- Collings A et al. Humoral and cellular immune responses to HIV-1 nef in mice DNA-immunised with non-replicating or self-replicating expression vectors. Vaccine 18:460-7 (1999). PubMed: 10519935
- Ranki A et al. Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS 9:1001-8 (1995). PubMed: 8527071
- Saito Y et al. Overexpression of nef as a marker for restricted HIV-1 infection of astrocytes in postmortem pediatric central nervous tissues. Neurology 44:474-81 (1994). PubMed: 8145918
- Ovod V et al. Immunological variation and immunohistochemical localization of HIV-1 Nef demonstrated with monoclonal antibodies. AIDS 6:25-34 (1992). PubMed: 1371924
- Zsolnai B et al. Effects of beta 2-adrenergic stimulants on the progesterone and oestradiol-17 beta serum levels in pseudopregnant rats. Acta Physiol Hung 77:7-11 (1991). PubMed: 1683091
