Cells transfected with plasmid encoding Nef and its mutants.
This antibody fails to detect Nef expressed in transfected cells. It cross-reacts with an unknown cellular protein of the same molecular weight (mock transfected cells as negative control) as well as the myoglobin red band of the molecular weight marker (SeeBlue Plus 2, Thermo Fisher).
Our Scientific Support team has resolved the issue in accordance with our Abpromise.
A free of charge replacement with the alternative ab42358 was sent and gives positive results.
Dr. Jason Lee
Submitted Aug 15 2016
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