Key features and details
- Guinea pig polyclonal to HIV1 Nef
- Suitable for: WB, ELISA
- Isotype: IgG
Product nameAnti-HIV1 Nef antibody
See all HIV1 Nef primary antibodies
DescriptionGuinea pig polyclonal to HIV1 Nef
Host speciesGuinea pig
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Other species
Recombinant Nef protein over-expressed in E. coli with a plasmid carrying the Nef coding region of HIV1 virus, subtype B.
- Extract of MT4 cells uninfected or infected with HIV1 (LAI strain).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Whole serum
Concentration information loading...
Purification notesab93919 is highly purified by several steps of chromatography.
Our Abpromise guarantee covers the use of ab63919 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 24 kDa.|
|ELISA||Use at an assay dependent dilution.|
RelevanceNef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation.
- C terminal core protein antibody
- F protein antibody
- Nef antibody
All lanes : Anti-HIV1 Nef antibody (ab63919) at 1/1000 dilution
Lane 1 : Extract of MT4 cells
Lane 2 : Extract of MT4 cells infected with HIV1 (LAI strain)
Predicted band size: 24 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
ab63919 has not yet been referenced specifically in any publications.