Anti-HIV1 Nef antibody [JR6] (ab42358)
Key features and details
- Mouse monoclonal [JR6] to HIV1 Nef
- Suitable for: WB, ELISA
- Reacts with: Human immunodeficiency virus
- Isotype: IgG1
Overview
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Product name
Anti-HIV1 Nef antibody [JR6]
See all HIV1 Nef primary antibodies -
Description
Mouse monoclonal [JR6] to HIV1 Nef -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Human immunodeficiency virus -
Immunogen
Recombinant Nef protein of HIV1 (B subtype consensus).
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Epitope
Mapped to amino acids 195-205 (ARELHPEYYKD - B subtype consensus) -
General notes
Also known as clone 01-009.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
IgG fraction, purified by affinty chromatography. -
Clonality
Monoclonal -
Clone number
JR6 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab42358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 0.2 µg/ml. Predicted molecular weight: 24 kDa.
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ELISA |
Use a concentration of 0.02 - 1 µg/ml.
Titer in ELISA/WB using rNEF of HIV 1 Subtype A. |
Notes |
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WB
Use a concentration of 0.2 µg/ml. Predicted molecular weight: 24 kDa. |
ELISA
Use a concentration of 0.02 - 1 µg/ml. Titer in ELISA/WB using rNEF of HIV 1 Subtype A. |
Target
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Relevance
Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. -
Database links
- Entrez Gene: 156110 Human immunodeficiency virus
- SwissProt: P03407 Human immunodeficiency virus
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Alternative names
- C terminal core protein antibody
- F protein antibody
- Nef antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab42358 has been referenced in 11 publications.
- Pushkarsky T et al. Abundance of Nef and p-Tau217 in Brains of Individuals Diagnosed with HIV-Associated Neurocognitive Disorders Correlate with Disease Severance. Mol Neurobiol 59:1088-1097 (2022). PubMed: 34843091
- Zhang HG et al. Ubiquitin E3 Ligase c-Cbl Is a Host Negative Regulator of Nef Protein of HIV-1. Front Microbiol 11:597972 (2020). PubMed: 33329486
- Lee JH et al. HIV Activates the Tyrosine Kinase Hck to Secrete ADAM Protease-Containing Extracellular Vesicles. EBioMedicine 28:151-161 (2018). PubMed: 29331674
- Lee JH et al. HIV-Nef and ADAM17-Containing Plasma Extracellular Vesicles Induce and Correlate with Immune Pathogenesis in Chronic HIV Infection. EBioMedicine 6:103-113 (2016). PubMed: 27211553
- Lee JH et al. HIV Nef, paxillin, and Pak1/2 regulate activation and secretion of TACE/ADAM10 proteases. Mol Cell 49:668-79 (2013). PubMed: 23317503