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  1. Link

    hiv1-nef-antibody-jr6-ab42358.pdf

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Cell Biology Apoptosis Receptors Death Receptors
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Anti-HIV1 Nef antibody [JR6] (ab42358)

  • Datasheet
  • SDS
Reviews (5) Submit a question References (10)

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Western blot - Anti-HIV1 Nef antibody [JR6] (ab42358)

    Key features and details

    • Mouse monoclonal [JR6] to HIV1 Nef
    • Suitable for: WB, ELISA, ICC/IF
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-HIV1 Nef antibody [JR6]
      See all HIV1 Nef primary antibodies
    • Description

      Mouse monoclonal [JR6] to HIV1 Nef
    • Host species

      Mouse
    • Tested applications

      Suitable for: WB, ELISA, ICC/IFmore details
    • Species reactivity

      Reacts with: Other species
    • Immunogen

      Recombinant Nef protein of HIV1 (B subtype consensus).

    • Epitope

      Mapped to amino acids 195-205 (ARELHPEYYKD - B subtype consensus)
    • General notes

      Also known as clone 01-009.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.40
      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Purification notes

      IgG fraction, purified by affinty chromatography.
    • Clonality

      Monoclonal
    • Clone number

      JR6
    • Isotype

      IgG1
    • Research areas

      • Cell Biology
      • Apoptosis
      • Receptors
      • Death Receptors
      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • ssRNA positive strand virus
      • HIV
      • Immunology
      • Immune System Diseases
      • Antiviral Signaling
      • Cancer
      • Oncoproteins/suppressors
      • Viral proteins
      • Retrovirus
      • Immunology
      • Immune System Diseases
      • Antiviral Signaling
      • HIV

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant HIV1 Nef protein (ab63996)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab42358 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    ELISA
    ICC/IF (1)
    Notes
    Application notes
    ELISA: 1/25000.
    ICC/IF: Use at an assay dependent dilution.
    RipA: Use at an assay dependent dilution.
    WB: 1/5000. Predicted molecular weight: 24 kDa.

    Titer in ELISA/WB using rNEF of HIV 1 Subtype A.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    Target

    • Relevance

      Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation.
    • Alternative names

      • C terminal core protein antibody
      • F protein antibody
      • Nef antibody
      • Negative factor antibody
      • p27 antibody
      see all

    Images

    • Western blot - Anti-HIV1 Nef antibody [JR6] (ab42358)
      Western blot - Anti-HIV1 Nef antibody [JR6] (ab42358)
      Lane 1 : Anti-HIV1 Nef antibody [JR6] (ab42358)


      Lane 1 : Recominant HIV1 Nef (B subtype)
      Lane 2 : Protein size marker

      Predicted band size: 24 kDa

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (10)

    Publishing research using ab42358? Please let us know so that we can cite the reference in this datasheet.

    ab42358 has been referenced in 10 publications.

    • Zhang HG  et al. Ubiquitin E3 Ligase c-Cbl Is a Host Negative Regulator of Nef Protein of HIV-1. Front Microbiol 11:597972 (2020). PubMed: 33329486
    • Lee JH  et al. HIV Activates the Tyrosine Kinase Hck to Secrete ADAM Protease-Containing Extracellular Vesicles. EBioMedicine 28:151-161 (2018). PubMed: 29331674
    • Lee JH  et al. HIV-Nef and ADAM17-Containing Plasma Extracellular Vesicles Induce and Correlate with Immune Pathogenesis in Chronic HIV Infection. EBioMedicine 6:103-113 (2016). PubMed: 27211553
    • Lee JH  et al. HIV Nef, paxillin, and Pak1/2 regulate activation and secretion of TACE/ADAM10 proteases. Mol Cell 49:668-79 (2013). PubMed: 23317503
    • Kjerrström A  et al. Interactions of single and combined human immunodeficiency virus type 1 (HIV-1) DNA vaccines. Virology 284:46-61 (2001). PubMed: 11352667
    • Collings A  et al. Humoral and cellular immune responses to HIV-1 nef in mice DNA-immunised with non-replicating or self-replicating expression vectors. Vaccine 18:460-7 (1999). PubMed: 10519935
    • Ranki A  et al. Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS 9:1001-8 (1995). PubMed: 8527071
    • Saito Y  et al. Overexpression of nef as a marker for restricted HIV-1 infection of astrocytes in postmortem pediatric central nervous tissues. Neurology 44:474-81 (1994). PubMed: 8145918
    • Ovod V  et al. Immunological variation and immunohistochemical localization of HIV-1 Nef demonstrated with monoclonal antibodies. AIDS 6:25-34 (1992). PubMed: 1371924
    • Zsolnai B  et al. Effects of beta 2-adrenergic stimulants on the progesterone and oestradiol-17 beta serum levels in pseudopregnant rats. Acta Physiol Hung 77:7-11 (1991). PubMed: 1683091

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-HIV1 Nef antibody [JR6]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (Huh7 transfected with Nef)
    Permeabilization
    No
    Specification
    Huh7 transfected with Nef
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 10 2021

    Western blot abreview for Anti-HIV1 Nef antibody [JR6]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293 T)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Treatment
    transfected with HIV1Nef
    Specification
    293 T
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 22 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-HIV1 Nef antibody [JR6]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Huh7, liver cell line)
    Permeabilization
    No
    Specification
    Huh7, liver cell line
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 22 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HIV1 Nef antibody [JR6]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 22 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-HIV1 Nef antibody [JR6]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Tonsil (HIV patient))
    Permeabilization
    No
    Specification
    Tonsil (HIV patient)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Acetone
    Read More

    HERR DR. Christian Ostalecki

    Verified customer

    Submitted Jun 13 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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