Anti-HIV1 Nef antibody [JR6] (ab42358)
- Datasheet
- References (8)
- Protocols
Overview
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Product name
Anti-HIV1 Nef antibody [JR6]
See all HIV1 Nef primary antibodies -
Description
Mouse monoclonal [JR6] to HIV1 Nef -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Recombinant Nef protein of HIV1 (B subtype consensus).
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Epitope
Mapped to amino acids 195-205 (ARELHPEYYKD - B subtype consensus) -
General notes
Also known as clone 01-009.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
IgG fraction, purified by affinty chromatography. -
Clonality
Monoclonal -
Clone number
JR6 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab42358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| ELISA | ||
| ICC/IF |
Application notes
ELISA: 1/25000.
ICC/IF: Use at an assay dependent dilution.
RipA: Use at an assay dependent dilution.
WB: 1/5000. Predicted molecular weight: 24 kDa.
Titer in ELISA/WB using rNEF of HIV 1 Subtype A.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
RipA: Use at an assay dependent dilution.
WB: 1/5000. Predicted molecular weight: 24 kDa.
Titer in ELISA/WB using rNEF of HIV 1 Subtype A.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. -
Alternative names
- C terminal core protein antibody
- F protein antibody
- Nef antibody
see all
Images
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![Western blot - Anti-HIV1 Nef antibody [JR6] (ab42358)](https://www.abcam.com/ps/products/42/ab42358/Images/ab42358-172786-anti-hiv1-nef-antibody-jr6-western-blot.jpg)
Western blot - Anti-HIV1 Nef antibody [JR6] (ab42358)Lane 1 : Anti-HIV1 Nef antibody [JR6] (ab42358)
Lane 1 : Recominant HIV1 Nef (B subtype)
Lane 2 : Protein size marker
Predicted band size: 24 kDa
References
This product has been referenced in:
- Lee JH et al. HIV Activates the Tyrosine Kinase Hck to Secrete ADAM Protease-Containing Extracellular Vesicles. EBioMedicine 28:151-161 (2018). Read more (PubMed: 29331674) »
- Lee JH et al. HIV-Nef and ADAM17-Containing Plasma Extracellular Vesicles Induce and Correlate with Immune Pathogenesis in Chronic HIV Infection. EBioMedicine 6:103-113 (2016). Read more (PubMed: 27211553) »
