Anti-HIV1 Nef antibody [JR6] (ab42358)
Key features and details
- Mouse monoclonal [JR6] to HIV1 Nef
- Suitable for: WB, ELISA, ICC/IF
- Isotype: IgG1
Overview
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Product name
Anti-HIV1 Nef antibody [JR6]
See all HIV1 Nef primary antibodies -
Description
Mouse monoclonal [JR6] to HIV1 Nef -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Recombinant Nef protein of HIV1 (B subtype consensus).
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Epitope
Mapped to amino acids 195-205 (ARELHPEYYKD - B subtype consensus) -
General notes
Also known as clone 01-009.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
IgG fraction, purified by affinty chromatography. -
Clonality
Monoclonal -
Clone number
JR6 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab42358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| ELISA | ||
| ICC/IF |
Application notes
ELISA: 1/25000.
ICC/IF: Use at an assay dependent dilution.
RipA: Use at an assay dependent dilution.
WB: 1/5000. Predicted molecular weight: 24 kDa.
Titer in ELISA/WB using rNEF of HIV 1 Subtype A.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
RipA: Use at an assay dependent dilution.
WB: 1/5000. Predicted molecular weight: 24 kDa.
Titer in ELISA/WB using rNEF of HIV 1 Subtype A.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. -
Alternative names
- C terminal core protein antibody
- F protein antibody
- Nef antibody
see all
Images
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![Western blot - Anti-HIV1 Nef antibody [JR6] (ab42358)](https://www.abcam.com/ps/products/42/ab42358/Images/ab42358-172786-anti-hiv1-nef-antibody-jr6-western-blot.jpg)
Western blot - Anti-HIV1 Nef antibody [JR6] (ab42358)Lane 1 : Anti-HIV1 Nef antibody [JR6] (ab42358)
Lane 1 : Recominant HIV1 Nef (B subtype)
Lane 2 : Protein size marker
Predicted band size: 24 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (9)
ab42358 has been referenced in 9 publications.
- Lee JH et al. HIV Activates the Tyrosine Kinase Hck to Secrete ADAM Protease-Containing Extracellular Vesicles. EBioMedicine 28:151-161 (2018). PubMed: 29331674
- Lee JH et al. HIV-Nef and ADAM17-Containing Plasma Extracellular Vesicles Induce and Correlate with Immune Pathogenesis in Chronic HIV Infection. EBioMedicine 6:103-113 (2016). PubMed: 27211553
- Lee JH et al. HIV Nef, paxillin, and Pak1/2 regulate activation and secretion of TACE/ADAM10 proteases. Mol Cell 49:668-79 (2013). PubMed: 23317503
- Kjerrström A et al. Interactions of single and combined human immunodeficiency virus type 1 (HIV-1) DNA vaccines. Virology 284:46-61 (2001). PubMed: 11352667
- Collings A et al. Humoral and cellular immune responses to HIV-1 nef in mice DNA-immunised with non-replicating or self-replicating expression vectors. Vaccine 18:460-7 (1999). PubMed: 10519935
- Ranki A et al. Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS 9:1001-8 (1995). PubMed: 8527071
- Saito Y et al. Overexpression of nef as a marker for restricted HIV-1 infection of astrocytes in postmortem pediatric central nervous tissues. Neurology 44:474-81 (1994). PubMed: 8145918
- Ovod V et al. Immunological variation and immunohistochemical localization of HIV-1 Nef demonstrated with monoclonal antibodies. AIDS 6:25-34 (1992). PubMed: 1371924
- Zsolnai B et al. Effects of beta 2-adrenergic stimulants on the progesterone and oestradiol-17 beta serum levels in pseudopregnant rats. Acta Physiol Hung 77:7-11 (1991). PubMed: 1683091
