Anti-HIV1 Nef antibody [JR6] (ab42358)
![Western blot - Anti-HIV1 Nef antibody [JR6] (ab42358)](https://www.abcam.com/ps/products/42/ab42358/Images/ab42358-172786-anti-hiv1-nef-antibody-jr6-western-blot.jpg "Western blot - Anti-HIV1 Nef antibody [JR6] (ab42358)")
Key features and details
- Mouse monoclonal [JR6] to HIV1 Nef
- Suitable for: WB, ELISA, ICC/IF
- Isotype: IgG1
Overview
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Product name
Anti-HIV1 Nef antibody [JR6]
See all HIV1 Nef primary antibodies -
Description
Mouse monoclonal [JR6] to HIV1 Nef -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Recombinant Nef protein of HIV1 (B subtype consensus).
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Epitope
Mapped to amino acids 195-205 (ARELHPEYYKD - B subtype consensus) -
General notes
Also known as clone 01-009.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
IgG fraction, purified by affinty chromatography. -
Clonality
Monoclonal -
Clone number
JR6 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab42358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) | |
| ELISA | ||
| ICC/IF | (1) |
| Notes |
|---|
Application notes
ELISA: 1/25000.
ICC/IF: Use at an assay dependent dilution.
RipA: Use at an assay dependent dilution.
WB: 1/5000. Predicted molecular weight: 24 kDa.
Titer in ELISA/WB using rNEF of HIV 1 Subtype A.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
RipA: Use at an assay dependent dilution.
WB: 1/5000. Predicted molecular weight: 24 kDa.
Titer in ELISA/WB using rNEF of HIV 1 Subtype A.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. -
Alternative names
- C terminal core protein antibody
- F protein antibody
- Nef antibody
see all
Images
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Western blot - Anti-HIV1 Nef antibody [JR6] (ab42358)Lane 1 : Anti-HIV1 Nef antibody [JR6] (ab42358)
Lane 1 : Recominant HIV1 Nef (B subtype)
Lane 2 : Protein size marker
Predicted band size: 24 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab42358 has been referenced in 10 publications.
- Zhang HG et al. Ubiquitin E3 Ligase c-Cbl Is a Host Negative Regulator of Nef Protein of HIV-1. Front Microbiol 11:597972 (2020). PubMed: 33329486
- Lee JH et al. HIV Activates the Tyrosine Kinase Hck to Secrete ADAM Protease-Containing Extracellular Vesicles. EBioMedicine 28:151-161 (2018). PubMed: 29331674
- Lee JH et al. HIV-Nef and ADAM17-Containing Plasma Extracellular Vesicles Induce and Correlate with Immune Pathogenesis in Chronic HIV Infection. EBioMedicine 6:103-113 (2016). PubMed: 27211553
- Lee JH et al. HIV Nef, paxillin, and Pak1/2 regulate activation and secretion of TACE/ADAM10 proteases. Mol Cell 49:668-79 (2013). PubMed: 23317503
- Kjerrström A et al. Interactions of single and combined human immunodeficiency virus type 1 (HIV-1) DNA vaccines. Virology 284:46-61 (2001). PubMed: 11352667
- Collings A et al. Humoral and cellular immune responses to HIV-1 nef in mice DNA-immunised with non-replicating or self-replicating expression vectors. Vaccine 18:460-7 (1999). PubMed: 10519935
- Ranki A et al. Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS 9:1001-8 (1995). PubMed: 8527071
- Saito Y et al. Overexpression of nef as a marker for restricted HIV-1 infection of astrocytes in postmortem pediatric central nervous tissues. Neurology 44:474-81 (1994). PubMed: 8145918
- Ovod V et al. Immunological variation and immunohistochemical localization of HIV-1 Nef demonstrated with monoclonal antibodies. AIDS 6:25-34 (1992). PubMed: 1371924
- Zsolnai B et al. Effects of beta 2-adrenergic stimulants on the progesterone and oestradiol-17 beta serum levels in pseudopregnant rats. Acta Physiol Hung 77:7-11 (1991). PubMed: 1683091