Product nameAnti-HIV1 Nef antibody [JR6]
See all HIV1 Nef primary antibodies
DescriptionMouse monoclonal [JR6] to HIV1 Nef
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Other species
Recombinant Nef protein of HIV1 (B subtype consensus).
EpitopeMapped to amino acids 195-205 (ARELHPEYYKD - B subtype consensus)
General notesAlso known as clone 01-009.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
PurityProtein A purified
Purification notesIgG fraction, purified by affinty chromatography.
Our Abpromise guarantee covers the use of ab42358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
RipA: Use at an assay dependent dilution.
WB: 1/5000. Predicted molecular weight: 24 kDa.
Titer in ELISA/WB using rNEF of HIV 1 Subtype A.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceNef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation.
- C terminal core protein antibody
- F protein antibody
- Nef antibody
This product has been referenced in:
- Lee JH et al. HIV Activates the Tyrosine Kinase Hck to Secrete ADAM Protease-Containing Extracellular Vesicles. EBioMedicine 28:151-161 (2018). Read more (PubMed: 29331674) »
- Lee JH et al. HIV-Nef and ADAM17-Containing Plasma Extracellular Vesicles Induce and Correlate with Immune Pathogenesis in Chronic HIV Infection. EBioMedicine 6:103-113 (2016). Read more (PubMed: 27211553) »