Overview

  • Product name

    Anti-HIV1 Nef antibody [JR6]
    See all HIV1 Nef primary antibodies
  • Description

    Mouse monoclonal [JR6] to HIV1 Nef
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Recombinant Nef protein of HIV1 (B subtype consensus).

  • Epitope

    Mapped to amino acids 195-205 (ARELHPEYYKD - B subtype consensus)
  • General notes

    Also known as clone 01-009.

Properties

Applications

Our Abpromise guarantee covers the use of ab42358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
ICC/IF
  • Application notes
    ELISA: 1/25000.
    ICC/IF: Use at an assay dependent dilution.
    RipA: Use at an assay dependent dilution.
    WB: 1/5000. Predicted molecular weight: 24 kDa.

    Titer in ELISA/WB using rNEF of HIV 1 Subtype A.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation.
    • Alternative names

      • C terminal core protein antibody
      • F protein antibody
      • Nef antibody
      • Negative factor antibody
      • p27 antibody
      see all

    Images

    • Lane 1 : Anti-HIV1 Nef antibody [JR6] (ab42358)


      Lane 1 : Recominant HIV1 Nef (B subtype)
      Lane 2 : Protein size marker

      Predicted band size: 24 kDa

    References

    This product has been referenced in:

    • Lee JH  et al. HIV Activates the Tyrosine Kinase Hck to Secrete ADAM Protease-Containing Extracellular Vesicles. EBioMedicine 28:151-161 (2018). Read more (PubMed: 29331674) »
    • Lee JH  et al. HIV-Nef and ADAM17-Containing Plasma Extracellular Vesicles Induce and Correlate with Immune Pathogenesis in Chronic HIV Infection. EBioMedicine 6:103-113 (2016). Read more (PubMed: 27211553) »
    See all 8 Publications for this product

    Customer reviews and Q&As

    Filter by Application

    Filter by Species

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    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Tonsil (HIV patient))
    Permeabilization
    No
    Specification
    Tonsil (HIV patient)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Acetone

    Dr. Christian Ostalecki

    Verified customer

    Submitted Jun 13 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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