Product nameAnti-HIV1 p15 antibody
DescriptionRabbit polyclonal to HIV1 p15
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Other species
Recombinant p15 protein (HIV1) expressed in E.coli with a plasmid carrying the Gag p15 coding region of HIV1 virus.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Whole serum
Our Abpromise guarantee covers the use of ab66951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).|
|ELISA||Use at an assay dependent dilution.|
RelevanceHIV1 p15(Gag) and its protease cleavage products, nucleocapsid protein p7 and p6, are believed to play a major role in viral infectivity and assembly during the early and late stages of retroviral life cycle. p15 is frequently recognized by HIV1-specific CD8+T cells in natural HIV1 infection and may be an important target for the design of future HIV1 vaccines.
Cellular localizationCytoplasmic and Nuclear
- gag antibody
- Gag polyprotein antibody
- Human Immunodeficiency virus 1 antibody
All lanes : Anti-HIV1 p15 antibody (ab66951) at 1/1000 dilution
Lane 1 : Molecular weight markers
Lane 2 : Extract of MT4 cells
Lane 3 : Extract of MT4 cells infected with HIV1
Predicted band size: 15 kDa
Observed band size: 15 kDa
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
This product has been referenced in:
- Lu B et al. Delivering SaCas9 mRNA by lentivirus-like bionanoparticles for transient expression and efficient genome editing. Nucleic Acids Res N/A:N/A (2019). Read more (PubMed: 30759231) »
- Garcia-Miranda P et al. Stability of HIV Frameshift Site RNA Correlates with Frameshift Efficiency and Decreased Virus Infectivity. J Virol 90:6906-17 (2016). Read more (PubMed: 27194769) »