Overview

  • Product name

    Anti-HIV1 p15 antibody
  • Description

    Rabbit polyclonal to HIV1 p15
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Recombinant p15 protein (HIV1) expressed in E.coli with a plasmid carrying the Gag p15 coding region of HIV1 virus.

Properties

Applications

Our Abpromise guarantee covers the use of ab66951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
ELISA Use at an assay dependent dilution.

Target

  • Relevance

    HIV1 p15(Gag) and its protease cleavage products, nucleocapsid protein p7 and p6, are believed to play a major role in viral infectivity and assembly during the early and late stages of retroviral life cycle. p15 is frequently recognized by HIV1-specific CD8+T cells in natural HIV1 infection and may be an important target for the design of future HIV1 vaccines.
  • Cellular localization

    Cytoplasmic and Nuclear
  • Alternative names

    • gag antibody
    • Gag polyprotein antibody
    • Human Immunodeficiency virus 1 antibody
    • Nucleocapsid protein p7 antibody
    • p15 antibody
    • p6 gag antibody
    • Pr55Gag antibody
    see all

Images

  • All lanes : Anti-HIV1 p15 antibody (ab66951) at 1/1000 dilution

    Lane 1 : Molecular weight markers
    Lane 2 : Extract of MT4 cells
    Lane 3 : Extract of MT4 cells infected with HIV1
    (LAI strain)

    Predicted band size: 15 kDa
    Observed band size: 15 kDa
    Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:

  • Lu B  et al. Delivering SaCas9 mRNA by lentivirus-like bionanoparticles for transient expression and efficient genome editing. Nucleic Acids Res N/A:N/A (2019). Read more (PubMed: 30759231) »
  • Garcia-Miranda P  et al. Stability of HIV Frameshift Site RNA Correlates with Frameshift Efficiency and Decreased Virus Infectivity. J Virol 90:6906-17 (2016). Read more (PubMed: 27194769) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

The lab has not tested specifically whether ab66951 binds to p6 vs p7, but they suspect due to the antibody being a polyclonal that it should recognize both p6 and p7. This antibody is the best option that we have for recognizing HIV1 p6.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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