Overview

  • Product name

    Anti-HIV1 p17 antibody [17-1]
    See all HIV1 p17 primary antibodies
  • Description

    Mouse monoclonal [17-1] to HIV1 p17
  • Host species

    Mouse
  • Specificity

    ab66641 recognsies bacterial HIV1 P17 Gag protein.
  • Tested applications

    Suitable for: WB, IP, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Bacterially expressed, hexahistidine amino-terminal tagged HIV-1 p17 Gag protein (clade B, HXB-3 isolate).

Properties

Applications

Our Abpromise guarantee covers the use of ab66641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Predicted molecular weight: 161 kDa.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    HIV1 p17 is the matrix protein of the Gag polyprotein which performs highly complex orchestrated tasks during the assembly, budding, maturation, and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA.
  • Cellular localization

    Matrix protein of HIV1 Gag polyprotein
  • Alternative names

    • HIV1 matrix protein p17 antibody
    • HIV1 p17 antibody
    • MA antibody
    • Matrix protein p17 antibody

References

ab66641 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us.

The answers to your questions are

Ab85536- to what extent the clade B is specific? Is there any crossreactivity?

A. We have note tested this antibody with HIV clade other than Clade B so unfortunately we are unable to share any data.

Ab30956-to what extent is this antibody specific?

A. We have note tested this antibody with HIV clade other than Clade B so unfortunately we are unable to share any data.

Ab66641- to what extent the clade B is specific?

A. We have note tested this antibody with HIV clade other than Clade B so unfortunately we are unable to share any data.

One of these antibodies is conjugated with His-Taq. What is better?Immunoprecopitation or isolation over the colony?

A. The immunogen of ab85536 is HIs tagged not the antibody. It will not affect the use of antibody in IP. You can certainly used this ab in IP.

Do you have any special immunoprecipitation protocols?

A. Customised protocols are not required for these product so lease try the standard protocols.
https://www.abcam.com/index.html?pageconfig=popular_protocols

Do you have protein A and protein G, that are used in immunoprecipitation?

A. We have protein A ab7399, and protein G, ab73758 as proteins. These products are not conjugated to any bead. You may need to get protein A or protein G conjugated beads for IP. Please check the IP protocol for more info.

Do you have some new products for immunoprecipitation for p55, glycoproteins gp41 and gp120

We do not have anti p55, gp41 and gp120 IP tested antibodies. However this does not mean these can't be used. If you are interested in using these in IP then let me know you may be eligible for Abreview testing discount. Please click the link for more info;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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