Key features and details
- Mouse monoclonal [38/8.7.47] to HIV1 p24
- Suitable for: Radioimmunoprecipitation, WB, ELISA, ICC/IF
- Reacts with: Species independent
- Isotype: IgG1
Product nameAnti-HIV1 p24 antibody [38/8.7.47]
See all HIV1 p24 primary antibodies
DescriptionMouse monoclonal [38/8.7.47] to HIV1 p24
SpecificityThis murine monoclonal antibody reacts with Human Immunodeficiency Virus Type 1(HIV 1) p24 protein.
Tested applicationsSuitable for: Radioimmunoprecipitation, WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Species independent
The antibody is used to detect HIV-1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis.
Antibody dilutions should be prepared using buffers containing suitable protein in order to stabilize antibody activity.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified from serum-free culture supernatant.
Primary antibody notesThe antibody is used to detect HIV-1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis.
Our Abpromise guarantee covers the use of ab9044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Radioimmunoprecipitation||Use at an assay dependent dilution.|
|WB||Use a concentration of 1 - 10 µg/ml.|
|ELISA||Use at an assay dependent dilution.|
|ICC/IF||Use a concentration of 1 - 10 µg/ml.|
RelevanceHIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
- CA antibody
- Capsid protein p24 antibody
- HIV-1 Gag p24 antibody
ab9044 has been referenced in 11 publications.
- Lee JH et al. HIV Activates the Tyrosine Kinase Hck to Secrete ADAM Protease-Containing Extracellular Vesicles. EBioMedicine 28:151-161 (2018). PubMed: 29331674
- Gohda J et al. BI-2536 and BI-6727, dual Polo-like kinase/bromodomain inhibitors, effectively reactivate latent HIV-1. Sci Rep 8:3521 (2018). PubMed: 29476067
- Reiser K et al. The Cellular Thioredoxin-1/Thioredoxin Reductase-1 Driven Oxidoreduction Represents a Chemotherapeutic Target for HIV-1 Entry Inhibition. PLoS One 11:e0147773 (2016). PubMed: 26816344
- Wasilewski LN et al. A Hepatitis C Virus Envelope Polymorphism Confers Resistance to Neutralization by Polyclonal Sera and Broadly Neutralizing Monoclonal Antibodies. J Virol 90:3773-82 (2016). PubMed: 26819308
- Mathys L & Balzarini J Several N-Glycans on the HIV Envelope Glycoprotein gp120 Preferentially Locate Near Disulphide Bridges and Are Required for Efficient Infectivity and Virus Transmission. PLoS One 10:e0130621 (2015). PubMed: 26121645