Anti-HIV1 p24 antibody [39/5.1.23] (ab9070)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-HIV1 p24 antibody [39/5.1.23]
See all HIV1 p24 primary antibodies -
Description
Mouse monoclonal [39/5.1.23] to HIV1 p24 -
Host species
Mouse -
Specificity
Reacts with Human Immunodeficiency Virus Type 1(HIV 1) p24 protein. -
Tested applications
Suitable for: WB, Radioimmunoprecipitation, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
General notes
The antibody is used to detect HIV 1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis.
Antibody dilutions should be prepared using buffers containing suitable protein in order to stabilize antibody activity.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
Purified from serum-free culture supernatant. -
Primary antibody notes
The antibody is used to detect HIV 1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis. -
Clonality
Monoclonal -
Clone number
39/5.1.23 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab9070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 10 µg/ml. | |
| Radioimmunoprecipitation | Use at an assay dependent dilution. | |
| ELISA | Use at an assay dependent dilution. | |
| ICC/IF | Use a concentration of 1 - 10 µg/ml. |
Target
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Relevance
HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex. -
Cellular localization
Membrane -
Alternative names
- CA antibody
- Capsid protein p24 antibody
- HIV-1 Gag p24 antibody
see all
Protocols
Datasheets and documents
References
This product has been referenced in:
- Zhou F et al. High-level expression of human immunodeficiency virus antigens from the tobacco and tomato plastid genomes. Plant Biotechnol J : (2008). WB . Read more (PubMed: 18771475) »
