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    hiv1-p24-antibody-395123-ab9070.pdf

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Microbiology Organism Virus RNA Virus ssRNA positive strand virus HIV
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Anti-HIV1 p24 antibody [39/5.1.23] (ab9070)

  • Datasheet
  • SDS
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Key features and details

  • Mouse monoclonal [39/5.1.23] to HIV1 p24
  • Suitable for: WB, Radioimmunoprecipitation, ELISA, ICC/IF
  • Reacts with: Species independent
  • Isotype: IgG1

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Recombinant HIV1 p24 protein (ab43037)

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Overview

  • Product name

    Anti-HIV1 p24 antibody [39/5.1.23]
    See all HIV1 p24 primary antibodies
  • Description

    Mouse monoclonal [39/5.1.23] to HIV1 p24
  • Host species

    Mouse
  • Specificity

    Reacts with Human Immunodeficiency Virus Type 1(HIV 1) p24 protein.
  • Tested applications

    Suitable for: WB, Radioimmunoprecipitation, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Species independent
  • General notes

    The antibody is used to detect HIV 1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis.

    Antibody dilutions should be prepared using buffers containing suitable protein in order to stabilize antibody activity.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Ammonium Sulphate Precipitation
  • Purification notes

    Purified from serum-free culture supernatant.
  • Primary antibody notes

    The antibody is used to detect HIV 1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis.
  • Clonality

    Monoclonal
  • Clone number

    39/5.1.23
  • Isotype

    IgG1
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • HIV
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant HIV1 p24 protein (ab43037)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 - 10 µg/ml.
Radioimmunoprecipitation
Use at an assay dependent dilution.
ELISA
Use at an assay dependent dilution.
ICC/IF
Use a concentration of 1 - 10 µg/ml.
Notes
WB
Use a concentration of 1 - 10 µg/ml.
Radioimmunoprecipitation
Use at an assay dependent dilution.
ELISA
Use at an assay dependent dilution.
ICC/IF
Use a concentration of 1 - 10 µg/ml.

Target

  • Relevance

    HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
  • Cellular localization

    Membrane
  • Alternative names

    • CA antibody
    • Capsid protein p24 antibody
    • HIV-1 Gag p24 antibody
    • HIV1gp1 antibody
    • Human immunodeficiency virus 1 antibody
    • Human immunodeficiency virus type 1 p24 antibody
    see all

Protocols

  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab9070? Please let us know so that we can cite the reference in this datasheet.

ab9070 has been referenced in 1 publication.

  • Zhou F  et al. High-level expression of human immunodeficiency virus antigens from the tobacco and tomato plastid genomes. Plant Biotechnol J : (2008). WB . PubMed: 18771475

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