Overview

  • Product name
    Anti-HIV1 p24 antibody [39/5.4A]
    See all HIV1 p24 primary antibodies
  • Description
    Mouse monoclonal [39/5.4A] to HIV1 p24
  • Host species
    Mouse
  • Specificity
    Human Immunodeficiency Virus Type 1(HIV 1) p24 protein. No detectable reaction has been observed with HIV-2 or SIV (Simian Immunodeficiency Virus) viral lysates by ELISA or Western blot.
  • Tested applications
    Suitable for: WB, Radioimmunoprecipitation, ELISA, ICC/IF, Sandwich ELISAmore details
  • Immunogen

    Tissue, cells or virus corresponding to HIV1 p24. ab9071 raised by immunizing BALB/c mice with purified HIV-1 (Strain IIIB) lysate.

  • General notes

    ab9071 is used to detect HIV-1 core protein in virus infected cells, cell lysates, culture fluid, serum or plasma.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified from serum-free culture supernatant.
  • Primary antibody notes
    The antibody is used to detect HIV 1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis. Useful as solid phase for capture of p24 in an antigen ELISA format.
  • Clonality
    Monoclonal
  • Clone number
    39/5.4A
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml.

ab9071 exhibits reactivity with viral lysates by Western blot
Radioimmunoprecipitation Use at an assay dependent concentration. The antibody immunoprecipitates p24 protein from radiolabeled infected cell extracts.
ELISA Use at an assay dependent concentration.

ab9071 is especially useful as a solid phase adsorbent or detector antibody in an ELISA format.
ICC/IF Use a concentration of 1 - 10 µg/ml. Exhibits reactivity with HIV-1 infected cultures using indirect immunofluorescence.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [39/6.14] to HIV1 p24 (ab9072).

Target

  • Relevance
    HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
  • Cellular localization
    Membrane
  • Alternative names
    • CA antibody
    • Capsid protein p24 antibody
    • HIV-1 Gag p24 antibody
    • HIV1gp1 antibody
    • Human immunodeficiency virus 1 antibody
    • Human immunodeficiency virus type 1 p24 antibody
    see all

Images

  • Western blot - Anti-HIV1 p24 antibody [39/5.4A] (ab9071)
    Western blot - Anti-HIV1 p24 antibody [39/5.4A] (ab9071)This image is courtesy of an anonymous Abreview
    All lanes : Anti-HIV1 p24 antibody [39/5.4A] (ab9071) at 1/2000 dilution

    Lane 1 : Mock transfected 293T cells - cell lysate
    Lane 2 : 293T cells transfected with HIV DNA - cell lysate

    Secondary
    All lanes : HRP conjugated goat anti-mouse IgG

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 24,25,41,55 kDa (why is the actual band size different from the predicted?)


    Exposure time: 5 minutes

    See Abreview

References

This product has been referenced in:
  • Taylor JP  et al. CRISPR/Cas9 knockout of USP18 enhances type I IFN responsiveness and restricts HIV-1 infection in macrophages. J Leukoc Biol N/A:N/A (2018). Read more (PubMed: 29437254) »
  • Ferdin J  et al. Viral protein Nef is detected in plasma of half of HIV-infected adults with undetectable plasma HIV RNA. PLoS One 13:e0191613 (2018). Read more (PubMed: 29364927) »

See all 42 Publications for this product

Publishing research using ab9071? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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19 reviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-HIV1 p24 antibody [39/5.4A]

 Excellent
Abreviews
Application
Western blot
Loading amount
0.5 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
HIV Purified protein (bacteria)
Specification
bacteria
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Read More
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Abcam user community

Verified customer

Submitted Apr 14 2014

Western blot abreview for Anti-HIV1 p24 antibody [39/5.4A]

 Excellent
Abreviews
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
HIV Cell lysate - whole cell (CEM-CCRF)
Specification
CEM-CCRF
Treatment
infected with NL.4-3 HIV-1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
abreview image
Username

Abcam user community

Verified customer

Submitted Feb 17 2015

Inquiry: Hi, Can you tell me from which subtype of HIV the p24 used to raise this antibody (and the sandwich partner ab0971) was taken? Thanks

Thank you for contacting us.

I can confirm that the Anti-HIV1 p24 antibody [39/5.4A] (ab9071) and the Anti-HIV1 p24 antibody [39/6.14] (ab9072) were made by injection of purified HIV-1 (Strain IIIB) lysate into mice.

I hope this infor...

Read More

do you have the epitope mapped for the monoclonal antibody?

Thank you for contacting us.

Sorry, we do not know the epitope of this antibody. We do know that it does not appear to react with HIV-2 or SIV antigen.

I hope this information is helpful to you. Please do not hesitate to contact us if...

Read More

Sorry, I meant HIV p24.

Thank you for contacting us.

I have good news and bad news for you:

We unfortunately cannot offer your a sELISA system in which the detection is conjugated either with HRP or Biotin.

The good news is that we indeed have m...

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I purchased ab9071 and ab9072 as they are noted on the Abcam website to be useful as a matched pair for sELISA. However the anti-mouse HRP secondary is binding to the capture antibody as well as the mouse detection antibody, producing a very high signa...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacements for one vial of ab63913 and one vial of ab16284 with the order number 1147505.
...

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Do you have any ELISA kit for detection HIV-1 P24 antigen human IgG ?
Sample : Human serum
Thank you.

Thank you for contacting us.

Unfortunately, we do not have a kit for this purpose, but we have two products which may interest you:

https://www.abcam.com/index.html?datasheet=9071 (or use the following: https://www.abcam.com/index....

Read More

Quiero pedir los anticuerpos ab9071 y ab83937. Cual es su precio y disponibilidad?
Como se realiza el pedido?
Muchas gracias.

Gracias por contactarnos.

Anti-HIV1 p24 [39/5.4A] (ab9071) tiene un precio de 370€ y se encuentra actualmente en stock, por lo que si lo pedís esta tarde o mañana (martes 12) a lo largo del día (antes de las 17 horas...

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Catalog number(s): 9071
Product(s): Mouse monoclonal [39/5.4A] to HIV1 p24
Survey results
----------------------------------------------------------------------------------
Telephone section
Q. Did you contact Abcam's Scientifi...

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Thank you for your participation in Abcam's post complaint survey.

I am sorry that the replacement antibody ab63958 did not work either.

Please let me know what problems you encountered (no bands as before?), and what antibody dilut...

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WB with p24 from HIV1 expressed in cells
WT and with mutation V86M
sees weak band at 41 and 55 kDa, but no band at 24 kDa
another Ab detects p24, though
loaded 50 ug protein
Ab: 1/500 o/n 4 degree
blocking: BSA or milk

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More

1-10 of 19 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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