Anti-HIV1 p24 antibody [39/5.4A] (ab9071)
- Datasheet
- References (42)
- Protocols
Overview
-
Product nameAnti-HIV1 p24 antibody [39/5.4A]
See all HIV1 p24 primary antibodies -
DescriptionMouse monoclonal [39/5.4A] to HIV1 p24
-
Host speciesMouse
-
SpecificityHuman Immunodeficiency Virus Type 1(HIV 1) p24 protein. No detectable reaction has been observed with HIV-2 or SIV (Simian Immunodeficiency Virus) viral lysates by ELISA or Western blot.
-
Tested applicationsSuitable for: WB, Radioimmunoprecipitation, ELISA, ICC/IF, Sandwich ELISAmore details
-
Immunogen
Tissue, cells or virus corresponding to HIV1 p24. ab9071 raised by immunizing BALB/c mice with purified HIV-1 (Strain IIIB) lysate.
-
General notes
ab9071 is used to detect HIV-1 core protein in virus infected cells, cell lysates, culture fluid, serum or plasma.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
-
Storage bufferConstituent: PBS
-
Concentration information loading... -
PurityProtein G purified
-
Purification notesPurified from serum-free culture supernatant.
-
Primary antibody notesThe antibody is used to detect HIV 1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis. Useful as solid phase for capture of p24 in an antigen ELISA format.
-
ClonalityMonoclonal
-
Clone number39/5.4A
-
IsotypeIgG1
-
Research areas
Associated products
-
Compatible Secondaries
-
Related Products
-
sELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab9071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 10 µg/ml. ab9071 exhibits reactivity with viral lysates by Western blot |
|
| Radioimmunoprecipitation | Use at an assay dependent concentration. The antibody immunoprecipitates p24 protein from radiolabeled infected cell extracts. | |
| ELISA | Use at an assay dependent concentration. ab9071 is especially useful as a solid phase adsorbent or detector antibody in an ELISA format. |
|
| ICC/IF | Use a concentration of 1 - 10 µg/ml. Exhibits reactivity with HIV-1 infected cultures using indirect immunofluorescence. | |
| Sandwich ELISA | Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [39/6.14] to HIV1 p24 (ab9072). |
Target
-
RelevanceHIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
-
Cellular localizationMembrane
-
Alternative names
- CA antibody
- Capsid protein p24 antibody
- HIV-1 Gag p24 antibody
see all
Images
-
![Western blot - Anti-HIV1 p24 antibody [39/5.4A] (ab9071)](https://www.abcam.com/ps/products/9/ab9071/Images/ab9071-320025-071.jpg)
Western blot - Anti-HIV1 p24 antibody [39/5.4A] (ab9071)Saito H. et al PLoS One. 2017 Sep 5;12(9):e0182434. doi: 10.1371/journal.pone.0182434. eCollection 2017.Immunoblot analysis of viral proteins in HEK293-derived cells transiently transfected with the proviral plasmid, pNL4-3. EV or POM121C (614–987) cells were transfected with 1.0 μg of pNL4-3. The cells were harvested 48 h post-transfection and lysates were subjected to immnoblot analyses with anti-HIV-1 p24 (top panel; the upper arrow indicated precursor Gag [PrGag], the lower arrow indicated CA), anti-HA (middle panel) and anti-CYPA (bottom panel). One representative set of results from three independent experiments is shown.
Whole-cell lysates were prepared as follows: cells were washed twice with phosphate-buffered saline (PBS) (-), suspended in PBS(-) (500 μl per 1 × 107 cells) and mixed with an equal volume of 2 × sample buffer (4% sodium dodecyl sulfate, 125 mM Tris-HCl, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol, and 0.002% bromphenol blue). Proteins were solubilized by heating for 5 min at 95°C. Samples were subjected to SDS-PAGE, transferred to PVDF membranes, and reacted with mouse monoclonal antibody to HIV-1 p24 (#ab9071, Abcam, Inc., Cambridge, MA), or rabbit polyclonal antibody to CYPA (#BML-SA296, Enzo Life Sciences, Inc., Farmingdale, NY). Membranes were then incubated with horseradish peroxidase-conjugated secondary antibody (#NA934 for anti-rabbit IgG, #NA931 for ant-mouse IgG, #NA933 for anti-human IgG, Amersham Biosciences, Piscataway, NJ), and proteins were visualized by Western Lightning Plus-ECL (PerkinElmer, Waltham, MA) or enhanced chemiluminescence (Pierce Biotechnology, Rockford, IL).
-
![Western blot - Anti-HIV1 p24 antibody [39/5.4A] (ab9071)](https://www.abcam.com/ps/products/9/ab9071/Images/ab9071_1.jpg)
Western blot - Anti-HIV1 p24 antibody [39/5.4A] (ab9071)This image is courtesy of an anonymous AbreviewAll lanes : Anti-HIV1 p24 antibody [39/5.4A] (ab9071) at 1/2000 dilution
Lane 1 : Mock transfected 293T cells - cell lysate
Lane 2 : 293T cells transfected with HIV DNA - cell lysate
Secondary
All lanes : HRP conjugated goat anti-mouse IgG
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 24,25,41,55 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
Protocols
References
This product has been referenced in:
- Taylor JP et al. CRISPR/Cas9 knockout of USP18 enhances type I IFN responsiveness and restricts HIV-1 infection in macrophages. J Leukoc Biol N/A:N/A (2018). Read more (PubMed: 29437254) »
- Ferdin J et al. Viral protein Nef is detected in plasma of half of HIV-infected adults with undetectable plasma HIV RNA. PLoS One 13:e0191613 (2018). Read more (PubMed: 29364927) »
