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Microbiology Organism Virus RNA Virus ssRNA positive strand virus HIV
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Anti-HIV1 p24 antibody [39/5.4A] (ab9071)

  • Datasheet
  • SDS
Reviews (8)Q&A (16)References (69)

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Western blot - Anti-HIV1 p24 antibody [39/5.4A] (ab9071)
  • Western blot - Anti-HIV1 p24 antibody [39/5.4A] (ab9071)

Key features and details

  • Mouse monoclonal [39/5.4A] to HIV1 p24
  • Suitable for: WB, Radioimmunoprecipitation, ELISA, ICC/IF, Sandwich ELISA
  • Reacts with: Species independent
  • Isotype: IgG1

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Overview

  • Product name

    Anti-HIV1 p24 antibody [39/5.4A]
    See all HIV1 p24 primary antibodies
  • Description

    Mouse monoclonal [39/5.4A] to HIV1 p24
  • Host species

    Mouse
  • Specificity

    Human Immunodeficiency Virus Type 1(HIV 1) p24 protein. No detectable reaction has been observed with HIV-2 or SIV (Simian Immunodeficiency Virus) viral lysates by ELISA or Western blot.
  • Tested applications

    Suitable for: WB, Radioimmunoprecipitation, ELISA, ICC/IF, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Tissue, cells or virus corresponding to HIV1 p24. ab9071 raised by immunizing BALB/c mice with purified HIV-1 (Strain IIIB) lysate.

  • General notes

    ab9071 is used to detect HIV-1 core protein in virus infected cells, cell lysates, culture fluid, serum or plasma.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from serum-free culture supernatant.
  • Primary antibody notes

    The antibody is used to detect HIV 1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis. Useful as solid phase for capture of p24 in an antigen ELISA format.
  • Clonality

    Monoclonal
  • Clone number

    39/5.4A
  • Isotype

    IgG1
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • HIV
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant HIV1 p24 protein (ab43037)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
  • sELISA pair antibody

    • Anti-HIV1 p24 antibody [39/6.14] (ab9072)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (6)
Use a concentration of 1 - 10 µg/ml.

ab9071 exhibits reactivity with viral lysates by Western blot

Radioimmunoprecipitation
Use at an assay dependent concentration. The antibody immunoprecipitates p24 protein from radiolabeled infected cell extracts.
ELISA
Use at an assay dependent concentration.

ab9071 is especially useful as a solid phase adsorbent or detector antibody in an ELISA format.

ICC/IF (1)
Use a concentration of 1 - 10 µg/ml. Exhibits reactivity with HIV-1 infected cultures using indirect immunofluorescence.
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [39/6.14] to HIV1 p24 (ab9072).
Notes
WB
Use a concentration of 1 - 10 µg/ml.

ab9071 exhibits reactivity with viral lysates by Western blot

Radioimmunoprecipitation
Use at an assay dependent concentration. The antibody immunoprecipitates p24 protein from radiolabeled infected cell extracts.
ELISA
Use at an assay dependent concentration.

ab9071 is especially useful as a solid phase adsorbent or detector antibody in an ELISA format.

ICC/IF
Use a concentration of 1 - 10 µg/ml. Exhibits reactivity with HIV-1 infected cultures using indirect immunofluorescence.
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [39/6.14] to HIV1 p24 (ab9072).

Target

  • Relevance

    HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
  • Cellular localization

    Membrane
  • Alternative names

    • CA antibody
    • Capsid protein p24 antibody
    • HIV-1 Gag p24 antibody
    • HIV1gp1 antibody
    • Human immunodeficiency virus 1 antibody
    • Human immunodeficiency virus type 1 p24 antibody
    see all

Images

  • Western blot - Anti-HIV1 p24 antibody [39/5.4A] (ab9071)
    Western blot - Anti-HIV1 p24 antibody [39/5.4A] (ab9071)Saito H. et al PLoS One. 2017 Sep 5;12(9):e0182434. doi: 10.1371/journal.pone.0182434. eCollection 2017. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunoblot analysis of viral proteins in HEK293-derived cells transiently transfected with the proviral plasmid, pNL4-3. EV or POM121C (614–987) cells were transfected with 1.0 μg of pNL4-3. The cells were harvested 48 h post-transfection and lysates were subjected to immnoblot analyses with anti-HIV-1 p24 (top panel; the upper arrow indicated precursor Gag [PrGag], the lower arrow indicated CA), anti-HA (middle panel) and anti-CYPA (bottom panel). One representative set of results from three independent experiments is shown.

    Whole-cell lysates were prepared as follows: cells were washed twice with phosphate-buffered saline (PBS) (-), suspended in PBS(-) (500 μl per 1 × 107 cells) and mixed with an equal volume of 2 × sample buffer (4% sodium dodecyl sulfate, 125 mM Tris-HCl, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol, and 0.002% bromphenol blue). Proteins were solubilized by heating for 5 min at 95°C. Samples were subjected to SDS-PAGE, transferred to PVDF membranes, and reacted with mouse monoclonal antibody to HIV-1 p24 (#ab9071, Abcam, Inc., Cambridge, MA), or rabbit polyclonal antibody to CYPA (#BML-SA296, Enzo Life Sciences, Inc., Farmingdale, NY). Membranes were then incubated with horseradish peroxidase-conjugated secondary antibody (#NA934 for anti-rabbit IgG, #NA931 for ant-mouse IgG, #NA933 for anti-human IgG, Amersham Biosciences, Piscataway, NJ), and proteins were visualized by Western Lightning Plus-ECL (PerkinElmer, Waltham, MA) or enhanced chemiluminescence (Pierce Biotechnology, Rockford, IL).

  • Western blot - Anti-HIV1 p24 antibody [39/5.4A] (ab9071)
    Western blot - Anti-HIV1 p24 antibody [39/5.4A] (ab9071)This image is courtesy of an anonymous Abreview
    All lanes : Anti-HIV1 p24 antibody [39/5.4A] (ab9071) at 1/2000 dilution

    Lane 1 : Mock transfected 293T cells - cell lysate
    Lane 2 : 293T cells transfected with HIV DNA - cell lysate

    Secondary
    All lanes : HRP conjugated goat anti-mouse IgG

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 24,25,41,55 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 minutes

    See Abreview

Protocols

  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (69)

    Publishing research using ab9071? Please let us know so that we can cite the reference in this datasheet.

    ab9071 has been referenced in 69 publications.

    • Joas S  et al. Nef-Mediated CD3-TCR Downmodulation Dampens Acute Inflammation and Promotes SIV Immune Evasion. Cell Rep 30:2261-2274.e7 (2020). PubMed: 32075764
    • Karadsheh R  et al. Composition and Orientation of the Core Region of Novel HIV-1 Entry Inhibitors Influences Metabolic Stability. Molecules 25:N/A (2020). PubMed: 32245167
    • Reiter K  et al. Separation of influenza virus-like particles from baculovirus by polymer-grafted anion exchanger. J Sep Sci 43:2270-2278 (2020). PubMed: 32187844
    • Kmiec D  et al. CpG Frequency in the 5' Third of the env Gene Determines Sensitivity of Primary HIV-1 Strains to the Zinc-Finger Antiviral Protein. mBio 11:N/A (2020). PubMed: 31937644
    • Imamichi H  et al. Defective HIV-1 proviruses produce viral proteins. Proc Natl Acad Sci U S A 117:3704-3710 (2020). PubMed: 32029589
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-HIV1 p24 antibody [39/5.4A]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    0.5 µg
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Sample
    HIV Purified protein (bacteria)
    Specification
    bacteria
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 14 2014

    Western blot abreview for Anti-HIV1 p24 antibody [39/5.4A]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Purified protein (eluted fractions from AKTA)
    Gel Running Conditions
    Reduced Denaturing (4-20% gel)
    Loading amount
    60 µg
    Specification
    eluted fractions from AKTA
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 16 2020

    Western blot abreview for Anti-HIV1 p24 antibody [39/5.4A]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human immunodeficiency virus Cell lysate - other (viral lysate)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    5 µg
    Specification
    viral lysate
    Blocking step
    Intercept Blocking Buffer as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 30% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 30 2020

    Western blot abreview for Anti-HIV1 p24 antibody [39/5.4A]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Purified protein (293T Cell supernatent)
    Gel Running Conditions
    Non-reduced Denaturing (10)
    Loading amount
    6e+006 cells
    Specification
    293T Cell supernatent
    Blocking step
    BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 08 2018

    Western blot abreview for Anti-HIV1 p24 antibody [39/5.4A]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Reduced Denaturing (10)
    Sample
    HIV Cell lysate - whole cell (CEM-CCRF)
    Specification
    CEM-CCRF
    Treatment
    infected with NL.4-3 HIV-1
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 17 2015

    Western blot abreview for Anti-HIV1 p24 antibody [39/5.4A]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (293T)
    Loading amount
    10000 cells
    Specification
    293T
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 17 2007

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