Overview

  • Product name
    Anti-HIV1 p24 antibody [39/5.4A]
    See all HIV1 p24 primary antibodies
  • Description
    Mouse monoclonal [39/5.4A] to HIV1 p24
  • Host species
    Mouse
  • Specificity
    Human Immunodeficiency Virus Type 1(HIV 1) p24 protein. No detectable reaction has been observed with HIV-2 or SIV (Simian Immunodeficiency Virus) viral lysates by ELISA or Western blot.
  • Tested applications
    Suitable for: WB, Radioimmunoprecipitation, ELISA, ICC/IF, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Tissue, cells or virus corresponding to HIV1 p24. ab9071 raised by immunizing BALB/c mice with purified HIV-1 (Strain IIIB) lysate.

  • General notes

    ab9071 is used to detect HIV-1 core protein in virus infected cells, cell lysates, culture fluid, serum or plasma.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified from serum-free culture supernatant.
  • Primary antibody notes
    The antibody is used to detect HIV 1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis. Useful as solid phase for capture of p24 in an antigen ELISA format.
  • Clonality
    Monoclonal
  • Clone number
    39/5.4A
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml.

ab9071 exhibits reactivity with viral lysates by Western blot

Radioimmunoprecipitation Use at an assay dependent concentration. The antibody immunoprecipitates p24 protein from radiolabeled infected cell extracts.
ELISA Use at an assay dependent concentration.

ab9071 is especially useful as a solid phase adsorbent or detector antibody in an ELISA format.

ICC/IF Use a concentration of 1 - 10 µg/ml. Exhibits reactivity with HIV-1 infected cultures using indirect immunofluorescence.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [39/6.14] to HIV1 p24 (ab9072).

Target

  • Relevance
    HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
  • Cellular localization
    Membrane
  • Alternative names
    • CA antibody
    • Capsid protein p24 antibody
    • HIV-1 Gag p24 antibody
    • HIV1gp1 antibody
    • Human immunodeficiency virus 1 antibody
    • Human immunodeficiency virus type 1 p24 antibody
    see all

Images

  • Immunoblot analysis of viral proteins in HEK293-derived cells transiently transfected with the proviral plasmid, pNL4-3. EV or POM121C (614–987) cells were transfected with 1.0 μg of pNL4-3. The cells were harvested 48 h post-transfection and lysates were subjected to immnoblot analyses with anti-HIV-1 p24 (top panel; the upper arrow indicated precursor Gag [PrGag], the lower arrow indicated CA), anti-HA (middle panel) and anti-CYPA (bottom panel). One representative set of results from three independent experiments is shown.

    Whole-cell lysates were prepared as follows: cells were washed twice with phosphate-buffered saline (PBS) (-), suspended in PBS(-) (500 μl per 1 × 107 cells) and mixed with an equal volume of 2 × sample buffer (4% sodium dodecyl sulfate, 125 mM Tris-HCl, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol, and 0.002% bromphenol blue). Proteins were solubilized by heating for 5 min at 95°C. Samples were subjected to SDS-PAGE, transferred to PVDF membranes, and reacted with mouse monoclonal antibody to HIV-1 p24 (#ab9071, Abcam, Inc., Cambridge, MA), or rabbit polyclonal antibody to CYPA (#BML-SA296, Enzo Life Sciences, Inc., Farmingdale, NY). Membranes were then incubated with horseradish peroxidase-conjugated secondary antibody (#NA934 for anti-rabbit IgG, #NA931 for ant-mouse IgG, #NA933 for anti-human IgG, Amersham Biosciences, Piscataway, NJ), and proteins were visualized by Western Lightning Plus-ECL (PerkinElmer, Waltham, MA) or enhanced chemiluminescence (Pierce Biotechnology, Rockford, IL).

  • All lanes : Anti-HIV1 p24 antibody [39/5.4A] (ab9071) at 1/2000 dilution

    Lane 1 : Mock transfected 293T cells - cell lysate
    Lane 2 : 293T cells transfected with HIV DNA - cell lysate

    Secondary
    All lanes : HRP conjugated goat anti-mouse IgG

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 24,25,41,55 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 minutes

    See Abreview

References

This product has been referenced in:
  • Taylor JP  et al. CRISPR/Cas9 knockout of USP18 enhances type I IFN responsiveness and restricts HIV-1 infection in macrophages. J Leukoc Biol N/A:N/A (2018). Read more (PubMed: 29437254) »
  • Ferdin J  et al. Viral protein Nef is detected in plasma of half of HIV-infected adults with undetectable plasma HIV RNA. PLoS One 13:e0191613 (2018). Read more (PubMed: 29364927) »
See all 45 Publications for this product

Customer reviews and Q&As

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1-5 of 5 Abreviews

Application
Western blot
Loading amount
0.5 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
HIV Purified protein (bacteria)
Specification
bacteria
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 14 2014

Application
Western blot
Sample
Human Purified protein (293T Cell supernatent)
Gel Running Conditions
Non-reduced Denaturing (10)
Loading amount
6e+006 cells
Specification
293T Cell supernatent
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Nov 08 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela)
Permeabilization
Yes - 0.1% triton
Specification
Hela
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 08 2018

Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
HIV Cell lysate - whole cell (CEM-CCRF)
Specification
CEM-CCRF
Treatment
infected with NL.4-3 HIV-1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 17 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (293T)
Loading amount
10000 cells
Specification
293T
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Jan 17 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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