Overview

  • Product name

    Anti-HIV1 p24 antibody [5]
    See all HIV1 p24 primary antibodies
  • Description

    Mouse monoclonal [5] to HIV1 p24
  • Host species

    Mouse
  • Specificity

    Reacts with HIV1 native p24 as well rp24 expressed in bacteria and Cos-7 cells. Reacts with rNef of HIV1 subtype B, C and A.
  • Tested applications

    Suitable for: ELISA, WB, ICC/IF, Radioimmunoprecipitationmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide:

    TPQDLNTMLNTVGGH

    , corresponding to amino acids 48-62 of HIV1 HXB2 p24.

Properties

Applications

Our Abpromise guarantee covers the use of ab63958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/25000.
WB 1/10000. Predicted molecular weight: 24 kDa.
ICC/IF Use at an assay dependent dilution.
Radioimmunoprecipitation Use at an assay dependent dilution.

Target

  • Relevance

    HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
  • Cellular localization

    Membrane
  • Alternative names

    • CA antibody
    • Capsid protein p24 antibody
    • HIV-1 Gag p24 antibody
    • HIV1gp1 antibody
    • Human immunodeficiency virus 1 antibody
    • Human immunodeficiency virus type 1 p24 antibody
    see all

Images

  • All lanes : Anti-HIV1 p24 antibody [5] (ab63958) at 1/1000 dilution

    All lanes : Hela cells infected with HIV1 (4 hours)

    Secondary
    All lanes : Goat anti-mouse conjugated to HRP at 1/2500 dilution

    Predicted band size: 24 kDa

    See Abreview

References

ab63958 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question

Catalog number(s): 9071
Product(s): Mouse monoclonal [39/5.4A] to HIV1 p24
Survey results
----------------------------------------------------------------------------------
Telephone section
Q. Did you contact Abcam's Scientific Support by telephone in reference to this complaint?
A. No
Q. Was it clear from the phone options which number you needed to press to get your issue resolved?
A.

Protocol advice section
Q. Did Abcam's Scientific Support give you protocol advice?
A.Yes
Q. How did you feel about being given protocol advice?
A.I was not expecting to be given protocol advice, but was happy to receive this
Q. Did the product work successfully after following the advice?
A.No
Q. Why have you not tried the protocol advice? (Select all that apply.)
A.

Free of charge replacement section
Q. Did Abcam's Scientific Support give you a free of charge replacement?
A. Yes

Q. Did the replacement product work successfully?
A.No
Credit note / refund section
Q. Did Abcam's Scientific Support give you a credit note or a refund?
A.No

Q. Would you have preferred a different outcome from receiving a credit note or refund?
A.
Overall satisfaction section
Q. How satisfied are you with how the complaint was handled?
A.Very satisfied
Q. Could we have done anything to better resolve the problem?
A.
Q. Would you mind if our scientific support team contacted you to better understand what happened and reach an agreeable solution?
A.No, I would not mind
Q. Please share with us any final thoughts you may have about Abcam, the complaints process, or our products.
A.

Read More
Answer

Thank you for your participation in Abcam's post complaint survey.

I am sorry that the replacement antibody ab63958 did not work either.

Please let me know what problems you encountered (no bands as before?), and what antibody dilution and incubation times you used. I'd be happy to discuss this further so that we can find an acceptable solution to this problem.

I look forward to hear back from you and assist you further.

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela cell line)
Loading amount
50 µg
Specification
Hela cell line
Gel Running Conditions
Reduced Denaturing (15% TrisHCl Criterion gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Nov 26 2008

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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