Overview

  • Product name

  • Description

    Rabbit polyclonal to HIV1 p24
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Recombinant p24 protein over-expressed in E.coli using a plasmid carrying the Gag p24 coding region of HIV1 virus, subtype B.

  • Positive control

    • Extract of MT4 cells infected with HIV-1 (LAI strain).

Properties

Applications

Our Abpromise guarantee covers the use of ab63913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
ELISA Use at an assay dependent dilution.

Target

  • Relevance

    HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
  • Cellular localization

    Membrane
  • Alternative names

    • CA antibody
    • Capsid protein p24 antibody
    • HIV-1 Gag p24 antibody
    • HIV1gp1 antibody
    • Human immunodeficiency virus 1 antibody
    • Human immunodeficiency virus type 1 p24 antibody
    see all

Images

  • All lanes : Anti-HIV1 p24 antibody (ab63913) at 1/2500 dilution

    Lane 1 : Extract of MT4 cells
    Lane 2 : Extract of MT4 cells infected with HIV-1 (LAI strain)

    Predicted band size: 24 kDa
    Observed band size: 24 kDa



    Bands at 41 and 55 kDa are also observed corresponding to HIV-p24 precursor proteins p41 and p55.

References

This product has been referenced in:

  • Parajuli B  et al. Restricted HIV-1 Env glycan engagement by lectin-reengineered DAVEI protein chimera is sufficient for lytic inactivation of the virus. Biochem J 475:931-957 (2018). Read more (PubMed: 29343613) »
  • Meuser ME  et al. Kinetic Characterization of Novel HIV-1 Entry Inhibitors: Discovery of a Relationship between Off-Rate and Potency. Molecules 23:N/A (2018). Read more (PubMed: 30081466) »
See all 9 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you for contacting us.

Sorry, we do not know the epitope of this antibody. We do know that it does not appear to react with HIV-2 or SIV antigen.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacements for one vial of ab63913 and one vial of ab16284 with the order number 1147505.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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