HRP Anti-HIV1 p24 antibody (ab20365)
Key features and details
- HRP Goat polyclonal to HIV1 p24
- Suitable for: ELISA, ICC, WB
- Reacts with: Human immunodeficiency virus
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-HIV1 p24 antibody
See all HIV1 p24 primary antibodies -
Description
HRP Goat polyclonal to HIV1 p24 -
Host species
Goat -
Conjugation
HRP -
Specificity
HIV1 p24 antibody (ab20365) targets p24 (predominate p24 band pattern in Western blot). Does not cross-react with human T or B cells. Antiserum is not reactive with human or bovine sera. -
Tested applications
Suitable for: ELISA, ICC, WBmore details -
Species reactivity
Reacts with: Human immunodeficiency virus -
Immunogen
Full length native protein (purified native p24 from strain IIIb).
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General notes
Purified IgG fraction covalently coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.002% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
IgG fraction -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Recombinant Protein
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA | (1) |
1/200 - 1/1000.
|
ICC |
1/20 - 1/200.
|
|
WB |
1/200 - 1/1000. Predicted molecular weight: 56 kDa. Dilution optimised using Chromogenic detection. When using chemiluminescent substrates, the assay will require much less antibody. Depending on the sensitivity of the substrate, the antibody dilution may be 1/10,000 - 1/100,000.
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Notes |
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ELISA
1/200 - 1/1000. |
ICC
1/20 - 1/200. |
WB
1/200 - 1/1000. Predicted molecular weight: 56 kDa. Dilution optimised using Chromogenic detection. When using chemiluminescent substrates, the assay will require much less antibody. Depending on the sensitivity of the substrate, the antibody dilution may be 1/10,000 - 1/100,000. |
Target
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Relevance
HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex. -
Cellular localization
Membrane -
Database links
- SwissProt: P04585 Human immunodeficiency virus
- SwissProt: P12497 Human immunodeficiency virus
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Alternative names
- CA antibody
- Capsid protein p24 antibody
- HIV-1 Gag p24 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab20365 has been referenced in 3 publications.
- Tan Z et al. Eliminating mesothelioma by AAV-vectored, PD1-based vaccination in the tumor microenvironment. Mol Ther Oncolytics 20:373-386 (2021). PubMed: 33614918
- Peyret H et al. Improving plant transient expression through the rational design of synthetic 5' and 3' untranslated regions. Plant Methods 15:108 (2019). PubMed: 31548848
- Guth CA & Sodroski J Contribution of PDZD8 to stabilization of the human immunodeficiency virus type 1 capsid. J Virol 88:4612-23 (2014). ELISA . PubMed: 24554657