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  1. Link

    hiv1-p24-elisa-kit-ab218268.pdf

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Microbiology Organism Virus RNA Virus ssRNA positive strand virus HIV
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SimpleStep

HIV1 p24 ELISA Kit (ab218268)

  • Datasheet
  • SDS
  • Protocol Booklet
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Other - HIV1 p24 ELISA Kit (ab218268)
  • Example of HIV1 p24 standard curve in Sample Diluent 50BS
  • Raw data values for Example of HIV-1 p24 standard curve in Sample Diluent 50BS.
  • Example of HIV1 p24 standard curve in Sample Diluent NS
  • Raw data values for example of HIV-1 p24 standard curve in Sample Diluent NS.
  • Example of HIV1 p24 standard curve in 1X Cell Extraction Buffer PTR
  • Example of HIV-1 p24 standard curve in 1X Cell Extraction Buffer PTR
  • Interpolated concentrations of spike HIV1 p24 in human serum, human plasmas, and rhesus macaque plasma
  • Interpolated concentrations of spike HIV1 p24 in Jurkat Cell Extract and RPMI + 10% FBS cell culture media
  • Serial dilutions of recombinant HIV1 p24 were prepared within the working range of the assay and assayed for reactivity.
  • Acid treatment recovery
  • Acid treatment effect
  • Acid treatment recovery of synthetic complexes
  • Linearity of dilution – spiked Purified HIV-1 p24 in Human serum, plasma (heparin, citrate, EDTA) and rhesus macaque EDTA plasma.
  • Linearity of dilution – spiked purified HIV-1 p24 in FBS cell culture media and Jurkat Cell Extract

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 1.1 pg/ml
  • Range: 4.69 pg/ml - 300 pg/ml
  • Sample type: Cell culture extracts, Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Overview

  • Product name

    HIV1 p24 ELISA Kit
    See all HIV1 p24 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 3 4.5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 5 4.7%
  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.1 pg/ml
  • Range

    4.69 pg/ml - 300 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 105 94% - 117%
    Cell culture extracts 107 103% - 113%
    Cell culture media 102 94% - 107%
    Hep Plasma 102 100% - 103%
    EDTA Plasma 98 93% - 103%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    HIV1 p24 ELISA Kit (ab218268) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of HIV1 p24 protein in human serum, plasma, cell culture supernatant, and cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate HIV1 p24 with 1.1 pg/mL sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips

    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.


    ASSAY SPECIFICITY This kit recognizes both native and recombinant HIV-1 p24 protein in serum, EDTA and heparin plasma, cell culture supernatant, and cell and tissue extract samples only. Urine, saliva, and milk samples have not been tested with this kit.


    CROSS REACTIVITY Recombinant HIV-1 Gag protein was prepared at 50 ng/mL and 1 ng/mL and assayed for cross reactivity. 1% cross-reactivity was observed.

  • Notes

    HIV1 p24 (capsid) protein is essential for HIV-1 viral replication and for the HIV-1 infection of non-dividing cells. HIV1 p24 proteins form viral capsid that encapsulates the genomic HIV1 RNA. HIV1 p24 concentration in host plasma is commonly used as indicator of viral load. Upon the viral infection, the development of anti-HIV1 p24 host humoral responses leads to immune complex formation and reduction of the free HIV1 p24 concentration in circulation.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X HIV1 p24 Capture Antibody 1 x 600µl
    10X HIV1 p24 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5BI 1 x 6ml
    HIV1 p24 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 50BS 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • HIV
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV
  • Relevance

    HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
  • Cellular localization

    Membrane
  • Alternative names

    • CA
    • Capsid protein p24
    • HIV-1 Gag p24
    • HIV1gp1
    • Human immunodeficiency virus 1
    • Human immunodeficiency virus type 1 p24
    see all

Images

  • Other - HIV1 p24 ELISA Kit (ab218268)
    Other - HIV1 p24 ELISA Kit (ab218268)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of HIV1 p24 standard curve in Sample Diluent 50BS
    Example of HIV1 p24 standard curve in Sample Diluent 50BS

    Background-subtracted data values (mean +/- SD) are graphed.

  • Raw data values for Example of HIV-1 p24 standard curve in Sample Diluent 50BS.
    Raw data values for Example of HIV-1 p24 standard curve in Sample Diluent 50BS.

    Raw data values are shown in the table

  • Example of HIV1 p24 standard curve in Sample Diluent NS
    Example of HIV1 p24 standard curve in Sample Diluent NS

    Background-subtracted data values (mean +/- SD) are graphed.

  • Raw data values for example of HIV-1 p24 standard curve in Sample Diluent NS.
    Raw data values for example of HIV-1 p24 standard curve in Sample Diluent NS.

    Raw data values are shown in the table

  • Example of HIV1 p24 standard curve in 1X Cell Extraction Buffer PTR
    Example of HIV1 p24 standard curve in 1X Cell Extraction Buffer PTR

    Background-subtracted data values (mean +/- SD) are graphed.

  • Example of HIV-1 p24 standard curve in 1X Cell Extraction Buffer PTR
    Example of HIV-1 p24 standard curve in 1X Cell Extraction Buffer PTR

    Raw data values are shown in the table

  • Interpolated concentrations of spike HIV1 p24 in human serum, human plasmas, and rhesus macaque plasma
    Interpolated concentrations of spike HIV1 p24 in human serum, human plasmas, and rhesus macaque plasma

    The concentrations of HIV1 p24 were measured in duplicates, interpolated from the HIV1 p24 standard curves and corrected for sample dilution. Undiluted samples are as follows: human serum 100% (neat), human plasma (heparin) 100% (neat), human plasma (EDTA) 100% (neat), and rhesus macaque plasma (EDTA) 100% (neat). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Interpolated concentrations of spike HIV1 p24 in Jurkat Cell Extract and RPMI + 10% FBS cell culture media
    Interpolated concentrations of spike HIV1 p24 in Jurkat Cell Extract and RPMI + 10% FBS cell culture media

    The concentrations of HIV1 p24 were measured in duplicates, interpolated from the HIV1 p24 standard curves and corrected for sample dilution. Undiluted samples are as follows: Jurkat cell extract 100 µg/mL, RPMI + 10% FBS cell culture media 100% (neat). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Serial dilutions of recombinant HIV1 p24 were prepared within the working range of the assay and assayed for reactivity.
    Serial dilutions of recombinant HIV1 p24 were prepared within the working range of the assay and assayed for reactivity.

    Serial dilutions of recombinant HIV1 p24 (group M, subtype B, strain 92418), HIV1 p24 (group M, subtype C, strain 92BR025) and HIV1 p24 (group O, strain BCF06) were prepared within the working range of the assay and assayed for reactivity. O.D. values within the linear range of each protein are graphed.

  • Acid treatment recovery
    Acid treatment recovery

    Three concentrations of purified HIV-1 p24 protein were spiked in duplicate to the indicated biological matrix and treated with the Acid Treatment Protocol to evaluate signal recovery in the working range of the assay. The signals of the same concentrations of purified HIV-1 p24 protein spiked in duplicate to Sample Diluent 50BS and treated with the Acid Treatment Protocol were taken as 100%.

  • Acid treatment effect
    Acid treatment effect

    Single concentrations of purified HIV-1 p24 protein were spiked in duplicate to the indicated biological matrix and treated with the Acid Treatment Protocol or mock acid treated to evaluate the effect of the Acid Treatment Protocol on the signal. The signals of single concentrations of purified HIV-1 p24 protein spiked in duplicate to the indicated biological matrix and mock acid treated were taken as 100%.

  • Acid treatment recovery of synthetic complexes
    Acid treatment recovery of synthetic complexes

    Single concentrations of purified HIV-1 p24 protein were spiked to the indicated biological matrix, pre-incubated with unlabeled capture and detector antibodies or unrelated antibodies and treated with the Acid Treatment Protocol to evaluate the signal recovery of p24 complexed with interfering antibodies by Acid Treatment Protocol. The signals of single concentrations of purified HIV-1 p24 protein spiked to the indicated biological matrix, preincubated with unrelated antibodies and treated with the Acid Treatment Protocol were taken as 100%. There were no signals if the single concentrations of purified HIV-1 p24 protein were spiked to the indicated biological matrix, pre-incubated with the unlabeled capture and detector antibodies and mock acid treated, indicating that the unlabeled capture and detector antibodies efficiently formed synthetic immune complexes with HIV-1 p24 and completely blocked the signals.

  • Linearity of dilution – spiked Purified HIV-1 p24 in Human serum, plasma (heparin, citrate, EDTA) and rhesus macaque EDTA plasma.
    Linearity of dilution – spiked Purified HIV-1 p24 in Human serum, plasma (heparin, citrate, EDTA) and rhesus macaque EDTA plasma.

    Purified HIV-1 p24 was spiked into biological samples and diluted in a 2-fold dilution series in Sample Diluent 50BS.

  • Linearity of dilution – spiked purified HIV-1 p24 in FBS cell culture media and Jurkat Cell Extract
    Linearity of dilution – spiked purified HIV-1 p24 in FBS cell culture media and Jurkat Cell Extract

    Purified HIV-1 p24 was spiked into biological samples and diluted in a 2-fold dilution series in Sample Diluent NS for cell culture media and 1X Cell Extraction Buffer PTR for cell extract samples.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (12)

Publishing research using ab218268? Please let us know so that we can cite the reference in this datasheet.

ab218268 has been referenced in 12 publications.

  • Yu J  et al. Deletion of the SARS-CoV-2 Spike Cytoplasmic Tail Increases Infectivity in Pseudovirus Neutralization Assays. J Virol N/A:N/A (2021). PubMed: 33727331
  • Cai J  et al. Infection with a newly designed dual fluorescent reporter HIV-1 effectively identifies latently infected CD4+ T cells. Elife 10:N/A (2021). PubMed: 33835029
  • Turkki V  et al. Experimental Evaluation of an Interferometric Light Microscopy Particle Counter for Titering and Characterization of Virus Preparations. Viruses 13:N/A (2021). PubMed: 34069520
  • Selberg S  et al. HIV Replication Is Increased by RNA Methylation METTL3/METTL14/WTAP Complex Activators. ACS Omega 6:15957-15963 (2021). PubMed: 34179640
  • Xu W  et al. Human immunodeficiency virus type 1 transcription is regulated by thieno[3,4- d ]pyrimidine. Exp Ther Med 19:3090-3096 (2020). PubMed: 32256797
View all Publications for this product

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