Anti-HIV1 p55 + p24 + p17 antibody (ab63917)
Key features and details
- Rabbit polyclonal to HIV1 p55 + p24 + p17
- Suitable for: WB, ELISA
- Reacts with: Human immunodeficiency virus
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-HIV1 p55 + p24 + p17 antibody -
Description
Rabbit polyclonal to HIV1 p55 + p24 + p17 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Human immunodeficiency virus -
Immunogen
Recombinant protein p55 over-expressed in E.coli using a plasmid carrying the Gag p55 coding region of HIV1 virus.
-
Positive control
- Extract of MT4 cells infected with HIV1 (LAI strain); HeLa whole cell lysate transfected with a plasmid expressing HIV1 gag.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.09% Sodium azide
Constituent: Whole serum -
Concentration information loading...
-
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/2000. Detects a band of approximately 17,24,55 kDa.
|
ELISA |
Use at an assay dependent concentration.
|
Notes |
---|
WB
1/2000. Detects a band of approximately 17,24,55 kDa. |
ELISA
Use at an assay dependent concentration. |
Target
-
Relevance
HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. p55 is digested by HIV1 protease into intermediate products p41 and p15. p41 is further digested into matrix protein p17 and capsid protein p24. Likewise, p15 is further digested into nucleocapsid protein p7 and to p6 and p1. -
Database links
- Entrez Gene: 155030 Human immunodeficiency virus
- SwissProt: P05888 Human immunodeficiency virus
- SwissProt: Q70622 Human immunodeficiency virus
- SwissProt: Q79665 Human immunodeficiency virus
-
Alternative names
- CA antibody
- Capsid protein p24 antibody
- Gag antibody
see all
Images
-
All lanes : Anti-HIV1 p55 + p24 + p17 antibody (ab63917) at 1/2000 dilution
Lane 1 : Extract of MT4 cells
Lane 2 : Extract of MT4 cells infected with HIV1
(LAI strain)
Observed band size: 17,24,55 kDa why is the actual band size different from the predicted?
Additional bands at: 39 kDa, 45 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-HIV1 p55 + p24 + p17 antibody (ab63917) at 1/2000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate transfected with a plasmid expressing HIV1 gag
Lane 2 : Untransfected HeLa whole cell lysate
Secondary
All lanes : Goat anti-rabbit IgG (whole molecule) HRP polymer at 1/50000 dilution
Datasheets and documents
-
Datasheet download
References (30)
ab63917 has been referenced in 30 publications.
- Sharma AL et al. Cocaine sensitizes the CD4+ T cells for HIV infection by co-stimulating NFAT and AP-1. iScience 25:105651 (2022). PubMed: 36483012
- Saccon E et al. Cytotoxic Lymphocytes Target HIV-1 Gag Through Granzyme M-Mediated Cleavage. Front Immunol 12:669347 (2021). PubMed: 33953729
- Rebensburg SV et al. Sec24C is an HIV-1 host dependency factor crucial for virus replication. Nat Microbiol 6:435-444 (2021). PubMed: 33649557
- Chia T et al. A Small Molecule, ACAi-028, with Anti-HIV-1 Activity Targets a Novel Hydrophobic Pocket on HIV-1 Capsid. Antimicrob Agents Chemother 65:e0103921 (2021). PubMed: 34228546
- Okoye I et al. Plasma Extracellular Vesicles Enhance HIV-1 Infection of Activated CD4+ T Cells and Promote the Activation of Latently Infected J-Lat10.6 Cells via miR-139-5p Transfer. Front Immunol 12:697604 (2021). PubMed: 34249000