Overview

  • Product name

    Anti-HIV1 Rev antibody [10]
    See all HIV1 Rev primary antibodies
  • Description

    Mouse monoclonal [10] to HIV1 Rev
  • Host species

    Mouse
  • Specificity

    HIV1 Rev regulatory protein.
  • Tested applications

    Suitable for: ELISA, WB, Radioimmunoprecipitationmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    To produce this antibody the recombinant Rev (strain BRU) has been used as immunogen. The peptide sequences were not derived from particular strains of HIV1, but were designed as (artificial) B-clade (strain) consensus sequences. Sequences in Swiss prot belong to particular HIV1 strains. For epitope mapping synthetic peptides were used.

  • Epitope

    Epitope mapped to aa34-40

Properties

Applications

Our Abpromise guarantee covers the use of ab25871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500. Predicted molecular weight: 13 kDa.
Radioimmunoprecipitation Use at an assay dependent dilution.

Target

  • Relevance

    HIV1 expresses the trans-activator protein Rev which is required for virus growth as it promotes the localisation and expression of viral RNA in the cytoplasm. Rev is a model experimental system for studying nuclear transport. The HIV1 Rev protein as viral protein is expressed early in the virus life cycle and thus may be important targets for the immune control of HIV1 infection.
  • Alternative names

    • Human immunodeficiency virus 1 Rev antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • Rev antibody

Images

  • Lane 1 : Anti-HIV1 Rev antibody [10] (ab25871)


    Lane 1 : Recombinant HIV1 Rev (B subtype)
    Lane 2 : Protein size marker

    Predicted band size: 13 kDa

References

This product has been referenced in:

  • Nomaguchi M  et al. Natural Single-Nucleotide Variations in the HIV-1 Genomic SA1prox Region Can Alter Viral Replication Ability by Regulating Vif Expression Levels. J Virol 90:4563-4578 (2016). Read more (PubMed: 26912631) »
  • Nomaguchi M  et al. Natural single-nucleotide polymorphisms in the 3' region of the hiv-1 pol gene modulate viral replication ability. J Virol 88:4145-60 (2014). WB . Read more (PubMed: 24478432) »
See all 2 Publications for this product

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